CLUSTAL W (1.83) multiple sequence alignment IRE2_Hsap MASAVRGSR-------------------------------------PWPR--L-GLQL-- IRE1_Hsap MPARRLLLL----------------------------------------------LTL-- ire-1_Dmel MRFCVVVCC-------------------------------------V--------LFL-- IRE2_Mmus MARPVQRFQ-------------------------------------LWSP--L-GFLL-- IRE1_Mmus MPARWLLLL----------------------------------------------LAL-- ire-1_Cele MRATFHLFT-------------------------------------F-------IFLL-- IRE1_Scer MRLLRRNML-------------------------------------VL-T----LLVC-- IreA_Ddis MTFSKTRNK-------------------------------------II-F--L-LFLI-- IrlA_Ddis MTKPIFKSK-------------------------------------TK-P--L-GWSK-- IrlB_Ddis MNSLLNNLI--------------------------------------------------- IrlB_C2d_Ddis MNSLLNNLI--------------------------------------------------- IrlC_Ddis MKSNYST---------------------------------------YL-Y--K-MSE--L IrlD_Ddis MGPKKGKRSHSKNHHHHNNGNNNNNNNSGGGSSSDILYTYSQDTRILL-F--K-VILTQN IrlE_Ddis MGKKKIDIN----------------------------------TREDL-F--L-SY---Y IrlF_Ddis MNFNFEQIL--------------------------------------------------- IrlF_C2d_Ddis MNFNFEQIL--------------------------------------------------- 10577_Tthe MTDLKVVVS----------------------------------------E--L-KF---- IRE1_Spur MF---------------------------------------------------------- CC1G_06931_Ccin MAFVFFLQF----------------------------------------------LLL-- AqueK159_Aque MLARDQPRD----------------------------------------------FTWL- AqueK160_Aque MLLVYRFSD-------------------------------------TF--------ESQ- AqueK161_Aque YD---------------------------------------------------------- AqueK162_Aque PESS-------------------------------------------------------- AqueK163_Aque MSSSQEVVA-------------------------------------SL-SHSLPLFIIE- AqueK164_Aque MYCQSLII---------------------------------------------------- AqueK165_Aque MVNLSRSLP---------------------------------------------EFLVD- AqueK166_Aque MTDPDP------------------------------------------------SFSIN- AqueK167_Aque MLELDSSLT------------------------------------------------IA- AqueK168_Aque MNSSVPWLL-------------------------------------L--------FSV-- AqueK169_Aque MAQLAVFDG-------------------------------------GR---I----LNI- IRE-2_Smoe MLKDFKPLF--------------------------------------------------- IRE-1_Smoe MLKDFKPLF--------------------------------------------------- IRE2_Hsap --Q-------F---AALLLGT----------------------L---------------- IRE1_Hsap --L-------L---PGL--GI----------------------F---------------- ire-1_Dmel --L-------A---SGLIASAT---AK-------------S-QQ---------------- IRE2_Mmus --Q-------L---VTL-LGK----------------------L---------------- IRE1_Mmus --L-------L---PPPGPGS----------------------F---------------- ire-1_Cele --L-------F---SSVIC-IS---TP-------------G-FR---------------- IRE1_Scer --V-------F---SSIIS-CS---IP-------------LSSR---------------- IreA_Ddis --I-------I---N-IF-NI----------------------N---------------- IrlA_Ddis --I-------S---S-K------------------------------------------- IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ERI-------V---NG----------------------------------GDV------- IrlD_Ddis EEIICMVIQIA---YGLP-GL----------------------PKISDISRDLGILILKL IrlE_Ddis K----------------------------------------------------------- IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe -----------------N-NE----------------------K---------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin --I-------V---GAVCWA-E---IV-------------N-VR---------------- AqueK159_Aque --S-------L---LASL------------------------------------------ AqueK160_Aque ------------QRI--------------------------------------------- AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque --E-------YEKLLAII-NIKLPPNPSQGPSHRFWDLFNA--H---------------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque --H-------YMRLMAVV-GVKSGLEPDWSLSRRFLELYST--H---------------- AqueK166_Aque --R-------VILIIY-------------------------------------------- AqueK167_Aque --T-------SPS----------------------------------------------- AqueK168_Aque --G-------F---FS-----C---LP-------------S-TD---------------- AqueK169_Aque ------------NDL--------------------------------------------- IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap SP---------------------------------------------------------- IRE1_Hsap GS---------------------------------------------------------- ire-1_Dmel GD-----------------------------------------------------S---- IRE2_Mmus GP---------------------------------------------------------- IRE1_Mmus GR---------------------------------------------------------- ire-1_Cele ND-----------------------------------------------------H---- IRE1_Scer TSRRQIVEDEVASTKKLNFNYGVDKNINSP---------------------IPAPRTT-- IreA_Ddis AY-------------------------------------------------I-------- IrlA_Ddis EV-------------------------------------------------I-------- IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis SSIFIEQDD--------------------------------------------------- IrlD_Ddis PNRTIRELELFLESKNIYFREDQHINYLNEIPQPTTTTTSPPPPPPPSSIST-------- IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe QQQ---------------DN-------------------------------Y-------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ST-------------------------------------------------IPAAETAAL AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque AA---------------------------------------------------------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque AS---------------------------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque SD---------------------------------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------QVH-TLRP---- IRE1_Hsap ------------------------------------------------TST-VTLP---- ire-1_Dmel -----------------------------------AEVVSSGEDEKTDCTDLARDE---- IRE2_Mmus ------------------------------------------------QVQ-SVRP---- IRE1_Mmus ------------------------------------------------TST-VTLP---- ire-1_Cele --------------------------------------------E--SIGDDEEKT---- IRE1_Scer --EGLPNMKLSSYPTPNLLNTADNRRANKKGRRAANSISV-PYLENRSLN--ELSL---- IreA_Ddis -------------------------------------------------KDE-NED---- IrlA_Ddis -------------------------------------------------NED-EEEEEGG IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis -------------------------------------------------TTT-TTT---- IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe -------------------------------------------------GME-DQHST-- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin IQRTRPA----------------------------PTYATGGILE--PTK--DLEL---- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque --------------------------------------------Q--KGS---------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque --------------------------------------------K--K------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque --------------------------------------------Q--SSSDIALAY---- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ---------------------------------------------------ENLLLVS-- IRE1_Hsap ---------------------------------------------------ETLLFVS-- ire-1_Dmel ---------------------------------------------------EALMVFS-- IRE2_Mmus ---------------------------------------------------ESLLFVS-- IRE1_Mmus ---------------------------------------------------ETLLFVS-- ire-1_Cele ---------------------------------------------------SSTILVS-- IRE1_Scer ---------------------------------------------------SDILIAA-- IreA_Ddis ---------------------------------------------------DLSLLIS-- IrlA_Ddis EEGGEEEEIDNNKNSNNSSSNSNNNNNDNNNNNGEERKVEKEEEKEEINHYRNQFEKD-- IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ---------------------------------------------------ATAIEIESS IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ---------------------------------------------------ELS-IAG-- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ---------------------------------------------------LDIVLVA-- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ---------------------------------------------------SLEVAVT-- AqueK164_Aque ---------------------------------------------------------D-- AqueK165_Aque ---------------------------------------------------SPQVAAS-- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ---------------------------------------------------KDLLLFS-- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe --------------------------------------------------------XX-- IRE-1_Smoe --------------------------------------------------------FQ-- IRE2_Hsap ------TLDGSLHALS-KQTG--------------------------------------- IRE1_Hsap ------TLDGSLHAVS-KRTG--------------------------------------- ire-1_Dmel ------TLGGGLTAID-PVTS--------------------------------------- IRE2_Mmus ------TLDGSLHALN-KQTG--------------------------------------- IRE1_Mmus ------TLDGSLHAVS-KRTG--------------------------------------- ire-1_Cele ------TIDGRLRALD-SETG--------------------------------------- IRE1_Scer ------DVEGGLHAVD-RRNG--------------------------------------- IreA_Ddis ------TLDGNIYSFN-YESG--------------------------------------- IrlA_Ddis ------LLDMELKALN-RETIDNILSGL-------------------------------- IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis SGLTSNITDSTEIQLD-STTTDTKTT-STTSTTTNNSSDSNNNNNNNNNNNSNNILNFPF IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------SIDGKFHALS-RSTG--------------------------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------YLYTILNGLEWKELA--------------------------------------- AqueK164_Aque ------QLDERA------------------------------------------------ AqueK165_Aque ------YVDKLLTGLGYEEMG--------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------TLDGKLYAVD-KVTG--------------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------XXXXXXXXXX-XXXX--------------------------------------- IRE-1_Smoe ------RTDYSLTCFE-VSTG--------------------------------------- IRE2_Hsap ----------------------DLKW--TLRDDPVIEG---------------------- IRE1_Hsap ----------------------SIKW--TLKEDPVLQV---------------------- ire-1_Dmel ----------------------EIRW--TIADDPPIVA---------------------- IRE2_Mmus ----------------------DLKW--TVKDDPIIQG---------------------- IRE1_Mmus ----------------------SIKW--TLKEDPVLQV---------------------- ire-1_Cele ----------------------EIKW--TLQEEPVLRS---------------------- IRE1_Scer ----------------------HIIW--SIEPENF----------Q-------------- IreA_Ddis ----------------------ELNW--DLKPNGGD----SLYST-SQFD---------- IrlA_Ddis ---------------NSYEIFKEVNF--DFEKLYSI----IISEPPPLFD--------T- IrlB_Ddis ---------------------IDYEY--DLIKVHHH----IQKN-IFIFP---------- IrlB_C2d_Ddis ---------------------IDYEY--DLIKVHHH----IQKN-IFIFP---------- IrlC_Ddis ----------------VLGEIKNFNY--DLYTKYDSL---VLKD-PQTQN---------- IrlD_Ddis NCQNKSKQKRPISKEESFNKMVQYNF--DLGKIFDE----FTRE-VLLDDLKNDLKIDNI IrlE_Ddis -------------------EIEKNKF--DLLKIC--------SD-KN------------- IrlF_Ddis ----------------------EYNF--DFKKLHKN----KLKN-NNLSE---------- IrlF_C2d_Ddis ----------------------EYNF--DFKKLHKN----KLKN-NNLSE---------- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------------------KILW--SMPAFARAEGSSTLSSLA-------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------ET---------------------- AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ----------------------KLKA--FIKNDVKVDV---------------------- AqueK164_Aque --------------------------------A--------------------------- AqueK165_Aque ----------------------EFSVYRYLHTEVDLDS---------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ----------------------DTLW--KLNSKSPIVT---------------------- AqueK169_Aque ------------------------------------N----------------------- IRE-2_Smoe ----------------------XXXX--XXXXXXXX------------------------ IRE-1_Smoe ----------------------SVKW--TIRFSEVA------------------------ IRE2_Hsap --------------------------PM-------------------------------- IRE1_Hsap --------------------------PT-------------------------------- ire-1_Dmel ---------------EH---------QE-------------------------------- IRE2_Mmus --------------------------PM-------------------------------- IRE1_Mmus --------------------------PT-------------------------------- ire-1_Cele --------------------------PS-------------------------------- IRE1_Scer ----------PLIE-IQE--------PS-------------------------------- IreA_Ddis --------------RKQQQ-STSTSTEI-------------------------------- IrlA_Ddis ------------FFDSVSS-FEIFFTDCLILLCSNFPDGIDTSI---------------- IrlB_Ddis --------------FKKQH-YHSTVLECLINLCSKLKYDENENLN--------------- IrlB_C2d_Ddis --------------FKKQH-YHSTVLECLINLCSKLKYDENENLN--------------- IrlC_Ddis ------------NSPKPPS-LEIFILVNLIALCGRI----NDKFP--------------- IrlD_Ddis PIDNISISPTPIISPVFQE-YDLFLMDSFIILCGQL----DNVKL--------------- IrlE_Ddis ------------FSKSNFL-KAVHCLFSLSYFCKIK--DENEEEEGKEEEINDYKVKKST IrlF_Ddis ---------------NEDD-YATTVIECLIHLCEVIPT--NKIY---------------- IrlF_C2d_Ddis ---------------NEDD-YATTVIECLIHLCEVIPT--NKIY---------------- 10577_Tthe ------------------------NQES-------------------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------PLVR-TTHPENDPDDFDD-------------------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque --------------------------HCTV------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque --------------------------HL-------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap -----------------------------------------YVT-EM-A---FLSDP-AD IRE1_Hsap -----------------------------------------HVE-EP-A---FLPDP-ND ire-1_Dmel -----------------------------------------NVQ-VP-H---FLPDP-RD IRE2_Mmus -----------------------------------------YVT-EM-A---FLSDP-AD IRE1_Mmus -----------------------------------------HVE-EP-A---FLPDP-ND ire-1_Cele -----------------------------------------AVKQGF-T---FLPNP-LD IRE1_Scer -----------------------------------------RLETYE-T---LIIEPFGD IreA_Ddis -----------------------------------------TKS-SP-S---ILIPT-LD IrlA_Ddis ---------------------------------TRPNFN-DFYN-HF-KDRGVINK---- IrlB_Ddis ---------------------------------SLDQFKIIFYN-ES-K---FLNKN-LK IrlB_C2d_Ddis ---------------------------------SLDQFKIIFYN-ES-K---FLNKN-LK IrlC_Ddis ---------------------------------NLDKFLTH-YK-YATNIFNLLSEN-II IrlD_Ddis ---------------------------------TLEEFKDY-YK-KY----KLLNQ---- IrlE_Ddis TGNYYRNSEGKLFKKSNEKDQIDDVSIFYKRLPTIQDFKDT-YD-KYEN---CLN----- IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe -----------------------------------------NFE-RY-D---YFLPS-SD IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin -----------------------------------------DT-TPE-T---YIIEP-QS AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque --------------------------------------------------------S-VD AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque -----------------------------------------SSS-SY-L---YLTDP-KD AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap G-----------------------------------------SLY-------ILGTQKQQ IRE1_Hsap G-----------------------------------------SLY-------TLGSKNNE ire-1_Dmel G-----------------------------------------SIY-------QLGQ--MG IRE2_Mmus G-----------------------------------------SLY-------VLGTQKQQ IRE1_Mmus G-----------------------------------------SLY-------TLGGKNNE ire-1_Cele G-----------------------------------------SLY-------VL---KNS IRE1_Scer G-----------------------------------------NIY-------YFNA--HQ IreA_Ddis G-----------------------------------------SGL-------LFQY-SND IrlA_Ddis -YIS----RENIHK-ALNDKLMTDYFNS--GIYLGLLDTLDI--AAIRFITIFGKF-LSY IrlB_Ddis EYLE----ILKNSK-EINKHVICDYFEK--D-NNGSEEKNQNSSIVFNFLNCFKDY-LSM IrlB_C2d_Ddis EYLE----ILKNSK-EINKHVICDYFEK--D-NNGSEEKNQNSSIVFNFLNCFKDY-LSM IrlC_Ddis NSIEKFKETLSKDLPSFQLSVIA----------NSSIVKKDN--SIIKFLSCFGSF-MKL IrlD_Ddis -YIEDFKDNLINDF-LVTVEILA----------SWYIPQKDY--SIVKFINCFGEM-LDS IrlE_Ddis SVYYIIRKIKLLHL-EYFWEILA----------SCY---GTI--SFIKFFNIFSNE-I-F IrlF_Ddis -------------------------------------------SGANKFISCFYEF-MNE IrlF_C2d_Ddis -------------------------------------------SGANKFISCFYEF-MNE 10577_Tthe G-----------------------------------------NFI-------FLAS-DST IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin G-----------------------------------------HIY-------VVNS-EDD AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque --------------------------------------------------------KVTE AqueK164_Aque DSLY----------------VWAGYQDGLPG-VHDSEEKRKVTSNIQHFTPSSGQW-IT- AqueK165_Aque --------------------------------------------------------KVNE AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque G-----------------------------------------SLY-------MSGP-QSD AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap G-LM-------KLPFT-------------------------------------------- IRE1_Hsap G-LT-------KLPFT-------------------------------------------- ire-1_Dmel S-LK-------KLPYT-------------------------------------------- IRE2_Mmus G-LM-------KLPFT-------------------------------------------- IRE1_Mmus G-LT-------KLPFT-------------------------------------------- ire-1_Cele S-LK-------KLPFN-------------------------------------------- IRE1_Scer G-LQ-------KLPLS-------------------------------------------- IreA_Ddis R-LH-------QVPFS-------------------------------------------- IrlA_Ddis EVVE-------TISNS-------LFRYRTDKIEF-----------------LSDIIRELL IrlB_Ddis DIIE-------FIVRGISFQVRSYCNLIKPIEQWKQKTYQQRYQQFKKALHLNNEVYKRI IrlB_C2d_Ddis DIIE-------FIVRGISFQVRSYCNLIKPIEQWKQKTYQQRYQQFKKALHLNNEVYKRI IrlC_Ddis ESIE-------YFGSV-------FNSLIGEEDQFSLDLLQ-------VLIDSSNEYKKFL IrlD_Ddis TIIE-------SFSYI-------FNKTIKKEDDWSISFIQ-------AALGSNEEFKRQV IrlE_Ddis SPSENGDSVFIMVGKF-------FRRKVRAETHYDLIFLK-------IIL-ANASI-PMI IrlF_Ddis RFIE-------TLVLE-------FYDLMTTD---NSKVTTGIFDQFFSLIKKSDIIYKSF IrlF_C2d_Ddis RFIE-------TLVLE-------FYDLMTTD---NSKVTTGIFDQFFSLIKKSDIIYKSF 10577_Tthe P-IK-------IMRDE-------------------------------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin S-LQ-------RFPFS-------------------------------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque K-VD-------H------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque A-LT-------RVKND-------------------------------------------- AqueK164_Aque ---R-------STTGTPPLGVRS------------------------------------- AqueK165_Aque K-LE-------KLMKF-------------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque G-IK-------RLPLT-------------------------------------------- AqueK169_Aque E-LI-------R------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis LCNEKY----RT----------------LSKSYDLVQKRLQKEEFTFIKVFEDDDPKLF- IrlB_Ddis VCNGVW----KSEKLDYI-YRYKS------------------DQLSLVGIIESDCSDLFI IrlB_C2d_Ddis VCNGVW----KSEKLDYI-YRYKS------------------DQLSLVGIIESDCSDLFI IrlC_Ddis QIL----------EFNQLQKYYD------------------DGHLCLFHSIKFDNLNEFK IrlD_Ddis MCL----------NDELIQDRYD------------------QSLYSIMHPIKFDNVREFI IrlE_Ddis LKLGVKDIGRFTEKEDII-VSYY-------------------RNFSIIHSLRFDNVKEFA IrlF_Ddis Y---EW----DSNILENR-FIFKNNNNNIDGDDDDGNEDDYFGRFSILSILEDDNSHII- IrlF_C2d_Ddis Y---EW----DSNILENR-FIFKNNNNNIDGDDDDGNEDDYFGRFSILSILEDDNSHII- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ----------------------------------------------------------IP IRE1_Hsap ----------------------------------------------------------IP ire-1_Dmel ----------------------------------------------------------IP IRE2_Mmus ----------------------------------------------------------IP IRE1_Mmus ----------------------------------------------------------IP ire-1_Cele ----------------------------------------------------------IP IRE1_Scer ----------------------------------------------------------IR IreA_Ddis ----------------------------------------------------------LQ IrlA_Ddis SALYQPPDIIKLITEP------------------------EKDSGFWCMIGIFKSKRILD IrlB_Ddis STLLID------SNSY------------------------EQLENVWRLICNFDSFSIAS IrlB_C2d_Ddis STLLID------SNSY------------------------EQLENVWRLICNFDSFSIAS IrlC_Ddis LLFN-D------STWCQQQ----------------QQQQQINPQIFWFILCKYNSSKIAH IrlD_Ddis LIYSRD------ENY-------------------------FEPQEFWYLICKYDAIAITQ IrlE_Ddis LVYKTL------KNNYPSKKKKKITLFEENGKINVKTS--KKDLMLFEFVCKFDCVKIFK IrlF_Ddis SNYFKF-------TNY------------------------ERLEDLWIMICHYQATDIAK IrlF_C2d_Ddis SNYFKF-------TNY------------------------ERLEDLWIMICHYQATDIAK 10577_Tthe ----------------------------------------------------------ID IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------------------------------------------------------MA AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ----------------------------------------------------------LP AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ----------------------------------------------------------RP AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ----------------------------------------------------------IP AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ELV-HASPCRSS-----------------------------DGVFYT------------G IRE1_Hsap ELV-QASPCRSS-----------------------------DGILYM------------G ire-1_Dmel QLV-ANAPCRSS-----------------------------DGILYS------------G IRE2_Mmus ELV-HASPCRSS-----------------------------DGVFYT------------G IRE1_Mmus ELV-QASPCRSS-----------------------------DGILYM------------G ire-1_Cele QLV-HASPCKGN-----------------------------DGILYA------------G IRE1_Scer QLV-STSPLHLKTN----------------IVVNDSGKIVEDEKVYT------------G IreA_Ddis ELV-NTSPLFLKELEDKSSTSSTSTTSESSK-------DENKVTMFI------------G IrlA_Ddis YLI-RTEPSKS---------------------------------WFK------------G IrlB_Ddis KFLIEFFKLDK-----------------------------------VNNKLNFSIHPSSD IrlB_C2d_Ddis KFLIEFFKLDK-----------------------------------VNNKLNFSIHPSSD IrlC_Ddis YLL-NDYQI------------------------------------------------NK- IrlD_Ddis HII-NNNIA------------------------------------------------EED IrlE_Ddis KYFLPKPQI-------------------------------------INNNDN-----NEM IrlF_Ddis ALF-NLYY---------------------------------------------------D IrlF_C2d_Ddis ALF-NLYY---------------------------------------------------D 10577_Tthe QVI-KICPMS-----------------------------GNESKYI-------------A IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ELV-DMSPFTSPG--------------------------DDEKRVFI------------G AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque KRI-I------------------------------------------------------- AqueK164_Aque ------YC---------------------CT-------SINDQLYYF------------G AqueK165_Aque AVL-Q------------------------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ELV-KISPCSSS-----------------------------DGLLYS------------G AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap R-KQDAWFVVDP-----ESGETQM----------------TLTTE--------------- IRE1_Hsap K-KQDIWYVIDL-----LTGEKQQ----------------TLSSAF-----AD------- ire-1_Dmel K-KSDTWYMVDP-----KTGRREK----------------VMGFGDA---TVD----GKE IRE2_Mmus R-KQDAWFVVDP-----ESGETQM----------------TLTTE--------------- IRE1_Mmus K-KQDIWYVIDL-----LTGEKQQ----------------TLSSAF-----AD------- ire-1_Cele S-KKDVWFGIDP-----KTGLKVEYILLNISDKILFLQVETLSSA-----SAD------- IRE1_Scer S-MRTIMYTINM-----LNGEIIS----------------AFGPGSKNGYFGSQSVDCSP IreA_Ddis N-KKTSITVVDS-----QTGEIIK----------------SMSKDGL---WLT------- IrlA_Ddis K-ESDYFFSI-------------------------------------------------- IrlB_Ddis A-NENYKR---------------------------------------------------- IrlB_C2d_Ddis A-NENYKR---------------------------------------------------- IrlC_Ddis ---KS-L----------------------------------------------------- IrlD_Ddis LSKRS-GKTY-------------------------------------------------- IrlE_Ddis LE-CI-SSGS-------------------------------------------------- IrlF_Ddis S-KEDCISVF-------------------------------------------------- IrlF_C2d_Ddis S-KEDCISVF-------------------------------------------------- 10577_Tthe F-TKEKILLVNP-----ENGELEK----------------FEDIEDD---YEN------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin R-KETSLLLLEL-----ETGRIKA----------------TLNSECP--PYDDRRIDLDE AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ----------DLGD---QLGE--------------------------------------- AqueK164_Aque G----------------------------------------------------------- AqueK165_Aque ----------VVRELCLCLHD--------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque S-KQDSWIAVDA-----LTGRKLY----------------SFSSHD----GMN------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap -------G-----PSTPRLYIGR-TQYTVTM---HDPRAP-----ALRWNTTYR-RYSAP IRE1_Hsap -----SLC-----PSTSLLYLGR-TEYTITM---YDTKTR-----ELRWNATYF-DYAAS ire-1_Dmel G---EQIGW----ATSRAIYLGR-TQYTVMM---YDSLAKNKD--AKPWNITFY-DYNAV IRE2_Mmus -------G-----LSTPQLFIGR-TQYTVSM---HDLRTP-----ALRWNTTYR-RYSAP IRE1_Mmus -----SLC-----PSTSLLYLGR-TEYTITM---YDTKTR-----ELRWNATYF-DYAAS ire-1_Cele -----RICPAN---QKQTIFLGR-TEYRVSM---FDEKNR-----GKTWNATFN-DYSAH IRE1_Scer E---EKIKL---QECENMIVIGK-TIFELGI---HSYD--G-----ASYNVTYS-T---- IreA_Ddis ---DEDDCPVNI-IPDEALMFTR-SDYQIIA---LDPKSG-----VEKWNLSVG-EYIPH IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ---Y-----------LNHYIINI-NQKLIKQ---LNIFTK-----RENWALTST-DVNLM IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin LEEPESRG-----SSTTEVFIGR-TDYYISI---YPHRSKGYGTRAPVQNLSFS-VYGPN AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque -------------------VIE--------------------------SIG-----SLH- AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------YQLSR-YRLAVSVLTNRDLISPGVP-----FNEVYE-DI--- AqueK164_Aque -------------------YCGHDYCYHNSI---TQLDTV-----SLQWRELEPTDATRP AqueK165_Aque ------------------YQVER-YRMVVTLLR-----RTALD-----SNKSFL-ELFMD AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque -----SMCPPNQYGSNKIIHIPS-IEYRVAV---LDSKTK-----QIKVNITYT-QYGTQ AqueK169_Aque -------------------LLER-YHFNKTL---YDRLG--LR-----LKL-FY-NTLEK IRE-2_Smoe -----------------------------------------------------------X IRE-1_Smoe -----------------------------------------------------------N IRE2_Hsap P----MDGSPGK------YM-SHLASCGMG-LLLTVDPG---SG---------------- IRE1_Hsap L----PEDDVDY------KM-SHFVSNGDG-LVVTVDSE---SG---------------- ire-1_Dmel S--APPELAKEY------EY-IHLTTTTNG-QIVTLDRK---HG---------------- IRE2_Mmus L----LNGSPGK------YM-SHLTSCGMG-LLLTVDPG---SG---------------- IRE1_Mmus L----PEDDVDY------KM-SHFVSNGDG-LVVTVDSE---SG---------------- ire-1_Cele L----LPEVNTW------PF-KHYASSSHG-YILTFDRE---TG---------------- IRE1_Scer ------------------------------------------------------------ IreA_Ddis S--T-----KSF------YN-SEISLNFEG-LIEVASLS--QRMYKIYIKKPEKTVVGIS IrlA_Ddis --------------------------------------------AEMGGKYF-------- IrlB_Ddis ---T-----KTS------TN-SNLSIDLVR-LLEFTTTNFSINVAKLLISKF-------- IrlB_C2d_Ddis ---T-----KTS------TN-SNLSIDLVR-LLEFTTTNFSINVAKLLISKF-------- IrlC_Ddis -----------K------QK-NPLEISPVT-LLEIAFLCFSDKIAIVLFSQ--------- IrlD_Ddis ---N-----SNL------SE-NPLEINPLS-LLEICSANFSHKIASILFQF--------- IrlE_Ddis ---Y-----SGF------DEKSKRKIR--I-FFSICCRQISPKILKCIVDY--------- IrlF_Ddis ---A-----SDD------NN-NKISISILR-ILEITSIEFSLPVLTVIVENT-------- IrlF_C2d_Ddis ---A-----SDD------NN-NKISISILR-ILEITSIEFSLPVLTVIVENT-------- 10577_Tthe I----------------------------------------------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin N--EDNLLQANYRRTSDDAY-IQGFPNGDV-YSFKTKSE---GGRV------------QT AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque L----LKKCG-------------------------------------------------- AqueK164_Aque VMRR-----GHG------GM-ISFEHDGVH-HLLMIGGG-------------------GG AqueK165_Aque L----TQELG-------------------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque S--------------------------------YTARQS---QG---------------- AqueK169_Aque I----KKDHG-------------------------------------------------- IRE-2_Smoe X----XX-XXXX------XX-X-XXXXXxSLTLLVSDGK---PS---------------- IRE-1_Smoe L----AE-DIDY------PG-I-AAGNTTSLTVLVSDGK---PS---------------- IRE2_Hsap TVLWTQD-LGVPVMGVYTWHQ-----------DGLRQLPHL------------------- IRE1_Hsap DVLWIQN-YASPVVAFYVWQR-----------EGLRKVMHI------------------- ire-1_Dmel KFLWQRD-LSSPVVAAFLLGP-----------DGLLSVPFT------------------- IRE2_Mmus IVLWTQD-LGVPVTGIYTWHQ-----------DGLHQLPHL------------------- IRE1_Mmus DVLWIQN-YASPVVAFYVWQG-----------EVLRKVVHI------------------- ire-1_Cele EMRWEQD-LKQPVVALYLLRD-----------DGLHKLPFE------------------- IRE1_Scer ---WQQNVLDVPLALQNTFSK-----------DGMCIAPFR------------------- IreA_Ddis HNYWEHI-LTSSPVSIYAYSS---------KKHILKKLDFHRKVSPYSNSLIPVASTDLM IrlA_Ddis ----NWT-LL-EPKLIKKEVN---------QKS--------------------------- IrlB_Ddis ------------PTFITSIPS---------RNS--------------------------- IrlB_C2d_Ddis ------------PTFITSIPS---------RNS--------------------------- IrlC_Ddis ------------FNYHELLPK---------KSK--------------------------- IrlD_Ddis ------------YDFKDSMPT---------RFS--------------------------- IrlE_Ddis ------------V------KD---------DIG--------------------------- IrlF_Ddis ------------PNFLNSIPT---------KTS--------------------------- IrlF_C2d_Ddis ------------PNFLNSIPT---------KTS--------------------------- 10577_Tthe --YED----------------------------FLYKGLQK------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin QPLWVQK-FHAPTVAVFDVLR---------GSTPLQPHPHA------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ---------SYPVAIAF------------------------------------------- AqueK164_Aque S----KP-AVQLPHYK--YIKKSNGNWRTNEHS----------MYNLSSRK--------- AqueK165_Aque ---------KYPLAVAL------------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque DKVWEKD-FGSPVVGIYSLEK---------S-QVLRSIPFT------------------- AqueK169_Aque KI---DD-CFIECLASW------------------------------------------- IRE-2_Smoe K----------------------------------------------------------- IRE-1_Smoe K----------------------------------------------------------- IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis IPSNFDRTFMFDDYYGQLFIVSPPSNNNN---NNNNNNNNN--NNNNNNNNNNNNNNNNN IrlA_Ddis -------------LKGVIPPR--ELNSQQRNTQFFRVFNGVTGFNDI------------- IrlB_Ddis -------------LWGIESRS--SLNPIE---NHFRILNCSFDFNNH------------- IrlB_C2d_Ddis -------------LWGIESRS--SLNPIE---NHFRILNCSFDFNNH------------- IrlC_Ddis -------------MKHLIKST--STLINN---NNNNNNNNNNNNNNNNNNNNNNNNNNNN IrlD_Ddis -------------LRGVFKPT--TTT-TT---TTTTTT-TTTTTIDKDE-KDKENNNNNV IrlE_Ddis -------------FLDLQNRQ--DI----------------------------------Y IrlF_Ddis -------------MLGMKFKL--HNNNNN---NNNNNNNNNNNNNNNNNNNNNNNNNNNN IrlF_C2d_Ddis -------------MLGMKFKL--HNNNNN---NNNNNNNNNNNNNNNNNNNNNNNNNNNN 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque -----------------------WSNP--------------------------------- AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis NNNNKNN-NNNNKNESDKSNLTPLTPYDPSKPNGANNNNNNNN--------------DL- IrlA_Ddis ------------------------------------------RFVYANP----------- IrlB_Ddis ----------------------------------------------------------Q- IrlB_C2d_Ddis ----------------------------------------------------------Q- IrlC_Ddis NSKNIG-LT----------------------------------------QYTVLEGYFLI IrlD_Ddis ISQQF---Q----------------------------------------YFRVLDVNCYF IrlE_Ddis ----IGDSA----------------------------------------PEKVLFLNCFF IrlF_Ddis NNNNINK-NNNDNGNGKAENIYRLSG-----DCKEKEKYDNNYSRETEIPFKVFDTKFL- IrlF_C2d_Ddis NNNNINK-NNNDNGNGKAENIYRLSG-----DCKEKEKYDNNYSRETEIPFKVFDTKFL- 10577_Tthe --------NQLTENEDYIFMQD-------------------------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque -----SI-IGQC------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis --LDSNKLKNYDIYLYSSIVILITSI---------------------------------- IrlA_Ddis --SLAQHAIKFIQKVTECGLFLNTDV---------------------------------- IrlB_Ddis --IPIDKQKDYINLFTKNLIHFNTEI---------------------------------- IrlB_C2d_Ddis --IPIDKQKDYINLFTKNLIHFNTEI---------------------------------- IrlC_Ddis NIRTRSRIAPFIEVLTMNGIHLTNDIIHTRIKSMSSDSIFRIFTEQYQQQQQNPLNNNQK IrlD_Ddis NDVIKKRAPIFIDLMTQSNIYLNTEV---------------------------------- IrlE_Ddis GNHKEQRVIDFFNLLTSKSIIICNTI---------------------------------- IrlF_Ddis --ITTMECSDYIRFFTSSSIIFNTEV---------------------------------- IrlF_C2d_Ddis --ITTMECSDYIRFFTSSSIIFNTEV---------------------------------- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis --------------------RNV--RDSIP-N---------------------------- IrlB_Ddis --------------------NEI--EDLIL-K---------------------------- IrlB_C2d_Ddis --------------------NEI--EDLIL-K---------------------------- IrlC_Ddis QIQFNENFTRQQKIDFDNCPNEI--DTFI-LDKTQCNLITLSQEFSIKVRAFTFYLSFGE IrlD_Ddis --------------------GEG--SDEN-VD---------------------------- IrlE_Ddis --------------------KEK--PEL--YD---------------------------- IrlF_Ddis --------------------NQTCYDDLKLIQ---------------------------- IrlF_C2d_Ddis --------------------NQTCYDDLKLIQ---------------------------- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------IP---------------------------- AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis -------------------------------------------SNS-----------FL- IrlB_Ddis -------------------------------------------ERY-----------HI- IrlB_C2d_Ddis -------------------------------------------ERY-----------HI- IrlC_Ddis IFTCSCEDYKREFSCKHMFFILLNYYHVPRNSYLIYQRQFTEIERYSIIMNIDLSKINSR IrlD_Ddis --------------------------------------------------------THES IrlE_Ddis -------------------------------------------YR-----YITLSTVDST IrlF_Ddis -------------------------------------------KRL-----------GF- IrlF_C2d_Ddis -------------------------------------------KRL-----------GF- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque -------------------------------------------PTY-----------GF- AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap -------------------------T--LA------------------------------ IRE1_Hsap -------------------------N--VA------------------------------ ire-1_Dmel -------------------------T--VS------------------------------ IRE2_Mmus -------------------------T--LA------------------------------ IRE1_Mmus -------------------------N--VA------------------------------ ire-1_Cele -------------------------V--MG------------------------------ IRE1_Scer -------------------------DKSLL------------------------------ IreA_Ddis -----------IVFIRSK---KNFNL--INV-----NNNNNQNNNQNSNQNNNINNKKT- IrlA_Ddis ----------------------FRKF--LEV-----NF------FYRYEKDNEVYQ---- IrlB_Ddis -----------SIRS-------------INN-----NNHDDDDYD-DYDDDDDHHSGCNN IrlB_C2d_Ddis -----------SIRS-------------INN-----NNHDDDDYD-DYDDDDDHHSGCNN IrlC_Ddis IYGTPNFNTFFKIKSSPSSTTSPFQS--INN-----NNNNNLNN---------------N IrlD_Ddis IYSFQN-----------------------------------------------------S IrlE_Ddis FYF-----------SDSH-KGADFGV--YTDEINVIINNDDIDYD-YIGD---------D IrlF_Ddis -----------LIYSNSNKFTYPFDF--WNN-----NNNNNNNNNNNNNNNNNNNNNNNN IrlF_C2d_Ddis -----------LIYSNSNKFTYPFDF--WNN-----NNNNNNNNNNNNNNNNNNNNNNNN 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin -------------------------F--VL------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque -----------ITEKI-------------------------------------------- AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque -------------------------S--IN------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis --------HQ---PTEENSL-----------LYSTSIISRIVEMEVTD------------ IrlB_Ddis NNNNNNDGDH---NEDENSIKEESL-IEFSNYKVDSMISRLLKYNAPS------------ IrlB_C2d_Ddis NNNNNNDGDH---NEDENSIKEESL-IEFSNYKVDSMISRLLKYNAPS------------ IrlC_Ddis NNNNLNNNNNNENENKFKEDGDDEILQNL--NRLNYILLRDFEFGPNDITTKLRETHINL IrlD_Ddis TEYLAINS---NSIHRLKRSS-------------ISLLSKVLEFND-------------- IrlE_Ddis DNDEEIE---ETKDYGSSSSDIDD-FYNVF-EPKASMIPNILDSCS-------------- IrlF_Ddis NNNNNNNNNN---NNNNNKNDDDEL-EKLVEYRKGSLISLVFSFAGYR------------ IrlF_C2d_Ddis NNNNNNNNNN---NNNNNKNDDDEL-EKLVEYRKGSLISLVFSFAGYR------------ 10577_Tthe ---------Q---HQDLKIKQKQSK-IKIVDFQTDQEI---------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ----------RDTLHFLAL--RWGHIRL-P-AS----GPRDTATLFSTLDTQ-------- IRE1_Hsap ----------VETLRYLTF--MSGEVGR---IT----KWKYPFPKETEAKSK-------- ire-1_Dmel ----------DEAYQAILE--ES-KTGN---V----------------NTVK-------- IRE2_Mmus ----------RDTLHFLVL--RWGHIRL-P-AS----SYQDTATQFSSLDTQ-------- IRE1_Mmus ----------VETLRYLTF--MSGEVGR---IT----KWKYPFPKETEAKSK-------- ire-1_Cele ----------KETMENVAK--NIFTVDQWPTVL----GVNAADPQTTSLTNQ-------- IRE1_Scer ----------ASDLDFRIA--RWVSPTF-P-------GI--------------------- IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------LLS--------VVLK---NLT----QQGCS----------LARE---- IrlB_Ddis -----------TTII--------ALLS---NLV----CNHMG----------VSQRILIL IrlB_C2d_Ddis -----------TTII--------ALLS---NLV----CNHMG----------VSQRILIL IrlC_Ddis FDRLLLIEDNLEALD--------KILK---NFT----QKGCT----------LSRD---- IrlD_Ddis ----------RDTLI--------SLLK---NLT----QGGCT----------LCKD---- IrlE_Ddis ----------LEILK--------AYIQ---NIT----GNGLF---------KISPS---- IrlF_Ddis -----------FFSS--------YIIE---NLT----GTNIG----------NPNYVFDI IrlF_C2d_Ddis -----------FFSS--------YIIE---NLT----GTNIG----------NPNYVFDI 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------LQ-----------------------------PRPRAEDVIPG-------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque -------------IIGVLERSGWGDTRR---LKPFADRSVDFNTRFSKVDLC-------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque -------------TIGVLEKSDWGDTGK---LKPFSLPNFDLNTSYPEVDLC-------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ----------DDTLQGMTA--SR--------AL----AIKNGLYSLTPTDTI-------- AqueK169_Aque -----------------LRKAD-----------GVETP---------TIDTL-------- IRE-2_Smoe ---------------------------------------------RKFQGTK-------- IRE-1_Smoe ---------------------------------------------RTFQGTK-------- IRE2_Hsap ----------LL-------MTLYV-GK--D--ETGF-YVSK-----AL------------ IRE1_Hsap ----------LT-------PTLYV-GK--Y--STSL-YASP-----SM------------ ire-1_Dmel ----------LF-------QSLYV-GE--H--QKGL-YALP-----SL------------ IRE2_Mmus ----------LL-------MTLYV-GK--E--EAGF-YVSK-----AL------------ IRE1_Mmus ----------LT-------PTLYV-GK--Y--STSL-YASP-----SM------------ ire-1_Cele ----------FF-------PALFV-GE--S--SFGL-YAIE-----AL------------ IRE1_Scer ----------------------IV-G--------LF-DVFN-----DL------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis --AVDERS--LL-------STIHS-DK--YYNSSIV-VLHNYWPIPPKFCDCSTSFISMA IrlB_Ddis STLIEEIQ--MI-------ESLLV-QN--DSFKTFM-LCESMVSTPNR------------ IrlB_C2d_Ddis STLIEEIQ--MI-------ESLLV-QN--DSFKTFM-LCESMVSTPNR------------ IrlC_Ddis --LVGDRN--LL-------VLVAILDS--TSKEKLS-LLNKYWPLPEK------------ IrlD_Ddis --LIDEKQ--LL-------ITISRPQY--HQSIQIL-HQNGYTIIPKY------------ IrlE_Ddis --LFLAHDVTRL-------VRNPKPKT--ETVVKILEFINTISRLSE------------- IrlF_Ddis SELMTDFE--II-------RGLLL-SK--Y--FSIL-FKSRKFTVPTL------------ IrlF_C2d_Ddis SELMTDFE--II-------RGLLL-SK--Y--FSIL-FKSRKFTVPTL------------ 10577_Tthe -------------------IAIFS-AV--G--FTNM-FQKN-----VI------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------LDRSLLRGAKSAYV-GMVEE--TGSL-YAMS-----PS------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque --------------------L--------------------------------------- AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ----------LT-------VADYY-GN--M--SDRD-FSSA-----KV------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ----------LT-------VAGYY-GY--M--RDID-FSNA-----KV------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ----------LQ-------SSFFV-GD--H--NGQM-YVMN-----AL------------ AqueK169_Aque ----------IA-------ALREI-GE--N------------------------------ IRE-2_Smoe ----------FT-------PIIVE-PP--Y------------------------------ IRE-1_Smoe ----------FT-------PIIVE-PP--Y------------------------------ IRE2_Hsap ---------VHT---GV-A---LVP----------------------------------- IRE1_Hsap ---------VHE---GV-A---VVP----------------------------------- ire-1_Dmel ---------VDK---NT-P---RIS----------------------------------- IRE2_Mmus ---------VHA---GV-A---LVP----------------------------------- IRE1_Mmus ---------VHE---GV-A---VVP----------------------------------- ire-1_Cele ---------VDH---QTIT---YSP----------------------------------- IRE1_Scer ---------RTN---EN-I---LVP----------------------------------- IreA_Ddis ------------------------------------------------------------ IrlA_Ddis NGKLPRTYSGSDGVIPYIFFARNH-LTLGF-----------LLSIH--NNITDTIDINHW IrlB_Ddis ---------WSKQSIPLVFFAYIGSDI--LNLKERPMFYK-FFDLNP-RLLLSIIP---- IrlB_C2d_Ddis ---------WSKQSIPLVFFAYIGSDI--LNLKERPMFYK-FFDLNP-RLLLSIIP---- IrlC_Ddis ---------TKKFAIPLIFFAIS-EECLK----------M-FIQISP-NLVKSTLNLHDW IrlD_Ddis -----------QLRDPYIFFAGS-KQILQ----------Y-LLEIHT-NSLSETIDIRHW IrlE_Ddis ---------LSTNGVPFTFLTNR-FEIVT----------SIIMSLHDERSISNSVEPVQW IrlF_Ddis ---------TRSTLIPILIFAFISNISYDMNCNIANVYLN-YLLLNR-ELYKGSIS---- IrlF_C2d_Ddis ---------TRSTLIPILIFAFISNISYDMNCNIANVYLN-YLLLNR-ELYKGSIS---- 10577_Tthe ---------VHNPLINTFISSEYDNTQ-----------------------------IQ-- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------HY---PLVAFG-DAE----------------------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ---------YT----SAVH---LK------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ---------YT----SSLH---LK------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ---------TEE---GSMQ---E------------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap --------------------------RGLTLAPADGPTTDEVTLQV------SG------ IRE1_Hsap --------------------------RGSTLPLLEGPQTDGVTIGD------KG------ ire-1_Dmel --------------------------TAPPIKLLDGPTGDQNSNQE------TD------ IRE2_Mmus --------------------------RGLTLAPMDGPTTDEVTLQV------SG------ IRE1_Mmus --------------------------RGSTLPLLEGPQTDGVTIGD------KG------ ire-1_Cele --------------------------KLLGPPLLEGPAPIALTEME------KE------ IRE1_Scer --------------------------HPFNPGDHESISSNKVYLDQ------TS------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ISVAISGGLYYDKEEIFRKYP-----------LLQ------------------KES---- IrlB_Ddis ------VPLI--FKTFLSCSDIIRNNFEDDLIN--------------------------- IrlB_C2d_Ddis ------VPLI--FKTFLSCSDIIRNNFEDDLIN--------------------------- IrlC_Ddis ISILTE-FALI-NETFLDHYPLLSAWY--------------------------------- IrlD_Ddis IQAIMSAGQYSSETEFFQYYPKIQELS--------------------------------- IrlE_Ddis LRALAP-FVSN-DEMKLIIYKV-RTLY-----N--------------------------- IrlF_Ddis ------ISQFGCIVNYLKCQNEIEKQYSGCLKLLEGLY---NH------KKEKSPNEPYR IrlF_C2d_Ddis ------ISQFGCIVNYLKCQNEIEKQYSGCLKLLEGLY---NH------KKEKSPNEPYR 10577_Tthe ------I---------------------------GGE------------KGIVSFNEGSN IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin --------------------------EGRKGRLIEGSGGSAQSIHDIIRNQKEA------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque --------------------------LPLNVPLIDGPQPQATSLDH-------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap -------------------------------EREGSPSTAVRY-PSGSV------ALPSQ IRE1_Hsap -------------------------------ECVITPSTDVKF-DPGLKSKNKLNYLRNY ire-1_Dmel -------------------------------PRTIYINDV---------------LQEHA IRE2_Mmus -------------------------------EREGSPSTAVRY-PSGSV------ALPSQ IRE1_Mmus -------------------------------ECVITPSTDLKF-DPGLKGKSKLNYLRNY ire-1_Cele -------------------------------EYLPPRRPIIRNIPPSITHKT----SDGE IRE1_Scer -----------------------NLSW-FALSSQNFPSLVESA-PISRY------ASSDR IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ----------------TLQKYPRPVNILEF-----------------QT------L---- IrlB_Ddis ----------------FEDDDSSSVNFFGFTSNFSNSDQ-DYL-SALET------CFENS IrlB_C2d_Ddis ----------------FEDDDSSSVNFFGFTSNFSNSDQ-DYL-SALET------CFENS IrlC_Ddis --------------------------------------S-DAR-SVETS------LFRLF IrlD_Ddis -------------------------------------LR-DKY-SRTLN------ILELQ IrlE_Ddis ----------------YEPHSN---------------GE-IKY-TELVR------LFDVQ IrlF_Ddis IDLLFGVINDRLKAPIFINKDKYESLYFVYQEDYQNPS---------------------- IrlF_C2d_Ddis IDLLFGVINDRLKAPIFINKDKYESLYFVYQEDYQNPS---------------------- 10577_Tthe FQLIPLQISTKLKNQCLENKQCRERL---------------------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin -------------------------------DRRIRCED---------------NP-RDR AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque --------------------------------NL-------------------------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque --------------------------------SH-------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ---------------------------------------------PS------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ----------------------------------------------------YLD----- IRE-1_Smoe ----------------------------------------------------YLD----- IRE2_Hsap WLL--------------------------------------------------------- IRE1_Hsap WLL--------------------------------------------------------- ire-1_Dmel GIM--------------------------------------------------------- IRE2_Mmus WLL--------------------------------------------------------- IRE1_Mmus WLL--------------------------------------------------------- ire-1_Cele YLL--------------------------------------------------------- IRE1_Scer WRV------------------SSIFEDETLFKNAIMGVHQIYNNEYDHLYENY------E IreA_Ddis -----------------------------------------------------------P IrlA_Ddis --L----------ENNECIEYIARTF--IKEIP-PHEVPTVKHSDLLILFEIS------V IrlB_Ddis N-V----------FNDQQLKQMARKL--LTFSK-RENQSRIAESELLEELELE------E IrlB_C2d_Ddis N-V----------FNDQQLKQMARKL--LTFSK-RENQSRIAESELLEELELE------E IrlC_Ddis TCM----------DKIDCINYLIQTY--LLPIP-TFKN-TLNNFETCYFFETY------F IrlD_Ddis YIL----------GHIEGIEFLLEKY--LDFTK-PY---HLDNQELLEIFDVT------F IrlE_Ddis YLLTKGKFSLSQFNGFETSIFLNPKL--LDGTI---------NSNILNSMIIK------D IrlF_Ddis ----------------DPFRVLPTRS--VDLSKISEGISRMKNDDFLNELEKE------E IrlF_C2d_Ddis ----------------DPFRVLPTRS--VDLSKISEGISRMKNDDFLNELEKE------E 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ECL--------------------------------------------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque --------------------------------S-VSNKNRIEFTLLLMKRNVISVGDVSK AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque --------------------------------N-VSNMSRVQFTRLLMERGVIEAGDVSK AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque --F--------------------------------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe YL---------------------------------------------------------- IRE-1_Smoe YL---------------------------------------------------------- IRE2_Hsap ------IGHHELPPV---------------------LH-----------T-T--MLR--V IRE1_Hsap ------IGHHETPLS---------------------AS-----------T-K--MLE--R ire-1_Dmel ------LGHYNMPNEGN--GNL--------QLSPTTAS-----------S-K--VVQ--S IRE2_Mmus ------IGYHEPPPV---------------------LH-----------T-T--MLR--V IRE1_Mmus ------IGHHETPLS---------------------AS-----------T-K--MLE--R ire-1_Cele ------LGYHDRPMM---------------------TM-----------ATI--IPT--R IRE1_Scer KTNSLDTTHKYPPLM---------------------ID-----------S-S--VDTTDL IreA_Ddis KKKK-------------------------------------------------------- IrlA_Ddis MKKR-------------------------------------------------------- IrlB_Ddis KRKK-------------------------------------------------------- IrlB_C2d_Ddis KRKK-------------------------------------------------------- IrlC_Ddis L----------------------------------------------------------- IrlD_Ddis VQKK-------------------------------------------------------- IrlE_Ddis KEKL-------------------------------------------------------- IrlF_Ddis KQKK-------------------------------------------------------- IrlF_C2d_Ddis KQKK-------------------------------------------------------- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------LGMHPLDETDGHESRMRRLLEGPSSISPIPTFRPGQNQWIDHGIEVVPNIE--A AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque IEDK----------------------------------------------------V--R AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque IEDK----------------------------------------------------D--R AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------SGHYPLPED---------------------SR-----------IK---IEQ--L AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe -----VKNHTLPPVS---------------------AP-----------S-N--AIL--A IRE-1_Smoe -----VKNHTLPPVS---------------------AP-----------S-N--AIL--A IRE2_Hsap HPTL-GSGTAE----------T-------------------------------------- IRE1_Hsap FPNN-LPKHRE----------N-------------------------------------- ire-1_Dmel LATI-HNYNDG----------Y-------------------------------------- IRE2_Mmus HPIP-GKVSAE----------T-------------------------------------- IRE1_Mmus FPNN-LPKHRE----------N-------------------------------------- ire-1_Cele YPVP-GPHK------------A-------------------------------------- IRE1_Scer HQNNEMNSLKE----------Y-------------------------------------- IreA_Ddis ----------K--KQKNK------------------------------------------ IrlA_Ddis --------------------SI-RSKE-------------SLQAELDLI--EEEKREKKK IrlB_Ddis ----------QKQQ-EKIKKRL-KREK-------------RLKEKQDLLLIKLEK----- IrlB_C2d_Ddis ----------QKQQ-EKIKKRL-KREK-------------RLKEKQDLLLIKLEK----- IrlC_Ddis ------------------------ENS-------------SIKAEQYLL--EQELL---- IrlD_Ddis -----------------D---I-RTEE-------------SLKAEKDLL--EQEEN---- IrlE_Ddis -----------------DLKELFFKEKPLSEAELKEKFEIATKNEKELL--DQLKKEN-- IrlF_Ddis ----------LKTKQKKKIKKL-ENEK-------------KQK----VL----------- IrlF_C2d_Ddis ----------LKTKQKKKIKKL-ENEK-------------KQK----VL----------- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin PPNN-SQQPAT----------P-------------------------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque YPIF-F---------KEYKKRTE------------------------------------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque YPLF-F---------KKYKKRCK------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque LSEK-WSQEKY----------R-------------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe LPSGSNPRNST------------------------------------------------- IRE-1_Smoe LPSGSNPRNST------------------------------------------------- IRE2_Hsap ---------------------------------------RPPEN--TQAPAFFLELLSLS IRE1_Hsap ---------------------------------------VIPAD--SEKK-SFEEVINLV ire-1_Dmel ---------------------------------------GLLAN--NEKN-AA------- IRE2_Mmus ---------------------------------------RASED--LHAPPVFFELLNLR IRE1_Mmus ---------------------------------------VIPAD--SEKR-SFEEVINIV ire-1_Cele ------------------------------------------------------------ IRE1_Scer ---------------------------------------MSPED--LEAYR--------- IreA_Ddis ------------------------------------------------------------ IrlA_Ddis QKDKKKNKSNQNQKNNNNQNNQSNNNKINSPSSNKLTQNVTPPS--SPVN-IITSSSTTS IrlB_Ddis QKEK-------------------------------------------------------- IrlB_C2d_Ddis QKEK-------------------------------------------------------- IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis QQDK-------------------------------------------------------- IrlF_C2d_Ddis QQDK-------------------------------------------------------- 10577_Tthe ---------------------------------------IFSVDKFDDKN-YIRDVMTCI IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ---------------------------------------VPPEE--VR------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap RE---------------KL-----------------WDSELHPEEKTPDSYLGLGP---- IRE1_Hsap DQTSENAPTTVSRDVEEKP-----------------AHAPARPEAP-----VDSML---- ire-1_Dmel DIGVQTDPELVEIGIDQRTNGNTINRTKTII-----LQNSNKVQAF-INEWFMEHP---- IRE2_Mmus RE-----------------------------------DPELHPEEKASDSYPGLGS---- IRE1_Mmus GQTSDNTPTTVSQDVEEKL-----------------ARAPAKPEAP-----VDSML---- ire-1_Cele -------------------IGSTIERPPPQL------LGPVEPQKHEDTSFILLLLN--- IRE1_Scer ---KKI-HEQISRELD----------------------------EKNQNSLL-LKF---- IreA_Ddis ------------------------------------------------------------ IrlA_Ddis SSTSSTTSS----TTSST-----IEPKPPTQTLPIKTSSPTKPESQKPSTPDPLIPKSSK IrlB_Ddis -----------------------------------------QLEEQMKKRE--KKL---- IrlB_C2d_Ddis -----------------------------------------QLEEQMKKRE--KKL---- IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------DA---- IrlF_Ddis -----------------------------------------QQEKEKKQNY--QQP---- IrlF_C2d_Ddis -----------------------------------------QQEKEKKQNY--QQP---- 10577_Tthe DNLRKGRMMIEVISDSKD-----I------------QNASN-----IPSQAFNFAK---- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin -----------------------------------------------K------T-G--- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque --------------------------------------NLL------------------- AqueK164_Aque ------------------------NNTRAVLFGG-------------------------- AqueK165_Aque --------------------------------------GP-------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque -------------------IGPTVQNK-----------EPL---QVVDGGH-IDGPG--- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe --------------------------------------NSGRGSFP-----VGWLF---- IRE-1_Smoe --------------------------------------NSGRGSFP-----VGWLF---- IRE2_Hsap ----------------------------------------QDLLA-ASLTAVL-LGGWI- IRE1_Hsap ----------------------------------------KDMAT-IILSTFL-LIGWV- ire-1_Dmel ----------------------------------------SGKVH-QILIVIV-LGMIA- IRE2_Mmus ----------------------------------------QDLLA-ATFTAIL-LGAWV- IRE1_Mmus ----------------------------------------KDMAT-IILSTFL-LVGWV- ire-1_Cele -----------------------------------------NHPI-PFYATLVTMFALL- IRE1_Scer ----------------------------------------GSLVYRIIETGVF-LLLFL- IreA_Ddis -----------------------NNKNNNDEDDENEIENYNDNQN------DL-I-DEF- IrlA_Ddis STTKNNNNNNNNNNNNNNNNNNNNNNNNNKENREMALEVINEMEK-IEIEESL-L-STS- IrlB_Ddis --------------------------------------------------QKL-E-RKR- IrlB_C2d_Ddis --------------------------------------------------QKL-E-RKR- IrlC_Ddis --------------------------------------------------------EKKK IrlD_Ddis --------------------------------------------------------EKKR IrlE_Ddis --------------------------------------------------EKL-K-KKNK IrlF_Ddis --------------------------------------------------QDL-Q-QHN- IrlF_C2d_Ddis --------------------------------------------------QDL-Q-QHN- 10577_Tthe ----------------------------------------DNKIT-IFLLTFL-IILVI- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------------------------------------WEYLG-VTLGLGL-VSLWF- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ----------------------------------------PDVLG-FSFSLLLVVAGTV- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ----------------------------------------GALLACLAVVTLV-LKGVL- IRE-1_Smoe ----------------------------------------GALLACLAVVTLV-LKGVL- IRE2_Hsap LFVM--R-----QQ-----------QPQVVE----------------------------- IRE1_Hsap AFII--TYPLSMHQ-----------QQQLQHQQFQKELEK-------------------- ire-1_Dmel LFWY--T----CST-----------MRELQ------------------------------ IRE2_Mmus LYLM--R-----Q----------------------------------------------- IRE1_Mmus AFII--TYPLSVHQ-----------QRQLQHQQFQKELEK-------------------- ire-1_Cele L-----TVIWQCGR-----------QWD-------------------------------- IRE1_Scer IFCA-------ILQ-----------RFKILP--------P-------------------- IreA_Ddis --IS--TN---SVI-----------QQQ-------------------------------- IrlA_Ddis SLVQ--PQ---QQS-----------QQSQQQQEQQQQQQQ-------------------- IrlB_Ddis LYKK--LQ---DDE-----------KQKLIEKQKQKEKEK-------------------- IrlB_C2d_Ddis LYKK--LQ---DDE-----------KQKLIEKQKQKEKEK-------------------- IrlC_Ddis QKEK---K---KQK-----------QKQSKS-KI-------------------------- IrlD_Ddis LKEK---R---KKE-----------EKEKKKQQN-------------------------- IrlE_Ddis LKKQ---K---NQQ-----------QQQQAKQQAQQQKQQ-------------------- IrlF_Ddis LKIQ--TQ---QQQ-----------QQQQQQQQQQQQQQQ-------------------- IrlF_C2d_Ddis LKIQ--TQ---QQQ-----------QQQQQQQQQQQQQQQ-------------------- 10577_Tthe ILIL--KD-ERITSMYKRNSGRSGGKSSSQKQNSQEEADK-------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin LFK-------KF---------------------GQREGFKVPKFIPVPNGDAQIARPLAP AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque -KE----M---N-------------PQHMVTPEQQQDTHL-------------------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque --------------------------------------TK-------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque VFSSLVASVVAVLV-----------TKS-------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe HF----------GQ------------KEKQKKEKE------------------------- IRE-1_Smoe HF----------GQ------------KEKQKKEKE------------------------- IRE2_Hsap -------------------------K--------------QQETPLAPAD---------- IRE1_Hsap -------------------I-QLL-QQQ------------QQQLPFHPPG---------- ire-1_Dmel -------------------------K--------------QSENGSKT------------ IRE2_Mmus ----------------------------------------QQQSPSAPAG---------- IRE1_Mmus -------------------I-QLL-QQQ--------------QLPFHPHG---------- ire-1_Cele ----------------------------------------QQKSTSRMDS---------- IRE1_Scer -------------------L-YVL------------------------------------ IreA_Ddis -----------------------Q-QQ-------------QQQ----------------- IrlA_Ddis -------------------Q-QQQ-QQQQQQQ-QQQQQQ-QQQQQQ-------------- IrlB_Ddis -------------------E-LSK-QKEKEFELLKQKEI-DEIKQKSIQE---------- IrlB_C2d_Ddis -------------------E-LSK-QKEKEFELLKQKEI-DEIKQKSIQE---------- IrlC_Ddis ------------------------IN--------------------NPLS---------- IrlD_Ddis ------------------------LKQKSLIT--------------NNTT----TTTTTT IrlE_Ddis -------------------H-QQNIQQNYENQHIEDQRKFNQQTKGRPIS---------- IrlF_Ddis -------------------Q-QQQ-QQQQQQQQQQQSQQ-QQQQQQSPQQ---------- IrlF_C2d_Ddis -------------------Q-QQQ-QQQQQQQQQQQSQQ-QQQQQQSPQQ---------- 10577_Tthe -------------------VLEKI-SKEQEEEIKKQNEQ-N-NKQE-------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin IPDTATEPAPAPEPATVQPL-QII-PEQ--------------PAPPAPLPLPLPP----- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque -------------------Y-TTT-TE--------------LSEST-------------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ----------------------VT-AP--------------ATDET-------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ----------------------------------------TQNVPHN------------- AqueK169_Aque -------------------------------------------AVA-------------- IRE-2_Smoe -------------------------QQQ------------KRKKRRGPK----------- IRE-1_Smoe -------------------------QQQ------------KRKKRRGPK----------- IRE2_Hsap ------FA---HISQD-------------------AQSLH-------------------- IRE1_Hsap -----------DTAQD-------------------GELLDTS------------------ ire-1_Dmel ------FA---------------------------------------------------- IRE2_Mmus ------PP---DLSQD-------------------AQGQL-------------------- IRE1_Mmus -----------DLTQD-------------------PEFLDSS------------------ ire-1_Cele ------FE---------------------------------------------------- IRE1_Scer ------------LSKI-------------------GFMPEKE------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------IDEINKNIQQ-------------------NQLKIDEINKKLEIKEIQNFQLPLL IrlB_C2d_Ddis ------IDEINKNIQQ-------------------NQLKIDEINKKLEIKEIQNFQLPLL IrlC_Ddis ------SSS--SSS----------------------SSPSTS-----------------N IrlD_Ddis PIPITVPIP--TQT----------------------QTPTQT-----------------P IrlE_Ddis ------PSS--IQNQN--------------------LNPTLL-----------------Q IrlF_Ddis ------QSQ--QQSQQ-------------------SQQKSQQQS---------------Q IrlF_C2d_Ddis ------QSQ--QQSQQ-------------------SQQKSQQQS---------------Q 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------LE---DPTTDALAGTGEEGDDTDREGDPEPE----------------------- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------PS---------------------------------------------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis NQQ-------------QQQPPPLQLLKQQQQQPS-----------SS---------LTIQ IrlB_C2d_Ddis NQQ-------------QQQPPPLQLLKQQQQQPS-----------SS---------LTIQ IrlC_Ddis TT---------------------------------------------------------- IrlD_Ddis TQTPIPTPIPTTPIPTTPIPIPIQLTPTTPKTPK-----------TPKTPKTPTTPK--- IrlE_Ddis NQ---------------------------------------------------------- IrlF_Ddis QKS-------------QQQSPPQQQ-QQQSQQPQQQSQQQSQQKQK-------------- IrlF_C2d_Ddis QKS-------------QQQSPPQQQ-QQQSQQPQQQSQQQSQQKQK-------------- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------AETKDD------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ----GPYSE--------------------------------------------------- ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ----GPFSE--------------------------------------------------- ire-1_Cele ------------------------------------------------------------ IRE1_Scer ----IPIVE--------------------------------------------------- IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis LESITPIL----------------------EPKNDNVVLLKSSIETPIDSVKKIQSIIEI IrlB_C2d_Ddis LESITPIL----------------------EPKNDNVVLLKSSIETPIDSVKKIQSIIEI IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ----TPITPKTPKNSTLDKQTISTPKTPPPL----------------------------- IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis -----------------------------------------------------HQKQQQQ IrlF_C2d_Ddis -----------------------------------------------------HQKQQQQ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ----DTASE--------------------------------------------------- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ----VPVQE--------------------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ---------------------------------------------------S--SGTSSP ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ---------------------------------------------------S--SGTSSP ire-1_Cele -----------------------------------------------------------I IRE1_Scer ---------------------------------------------------S--KSLNCP IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis QLEEENQKKKEMETIDEYEEININKKQINSNMKWGGIGEPSWISVKKDNKKSK------- IrlB_C2d_Ddis QLEEENQKKKEMETIDEYEEININKKQINSNMKWGGIGEPSWISVKKDNKKSK------- IrlC_Ddis ------------------------------------------------------ITSTTP IrlD_Ddis -------------------------------------------------SVNTTPITPSP IrlE_Ddis ------------------------------------------------------NQTSNP IrlF_Ddis QKQEKQQQKQE--KQQQKQ----------------------------------------- IrlF_C2d_Ddis QKQEKQQQKQE--KQQQKQ----------------------------------------- 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin -----------------------------------------------------------T AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ---------------------------------------------------C--TEDSQS AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ---------------------------------------------------R--KAQATS AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque -----------------------------------------------------------P AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ----------------------------------SG----------------AS-RRSQK IRE1_Hsap STS--------PR----------------ASNH-SL----------------CS-GSSAS ire-1_Dmel -IA--------QN----------------GSN-----------------------GSTGS IRE2_Mmus ----------------------------------SR----------------DI-LQDQR IRE1_Mmus SPS--------PR----------------ASNH-SL----------------HP-SSSAS ire-1_Cele VNN--------PG----------------ESRS--A----------------QT-SK-QS IRE1_Scer SSSENVT---KPFDMKSGKQVVFEGAVNDGSLK-SEKDNDDADEDDEKSLDLTTEKKKRK IreA_Ddis ------------------------------------------------------------ IrlA_Ddis -------------------------------EKQ---Q---------------------- IrlB_Ddis ---STITTSTTPT---T-------------TTPA---KVITSQFKNEQ------------ IrlB_C2d_Ddis ---STITTSTTPT---T-------------TTPA---KVITSQFKNEQ------------ IrlC_Ddis TTTTTTTTTTTPT---T-------------TTTT---TTT-SSP-KQKP----------- IrlD_Ddis PPQTIATT-ITTT---T-------------TTPK---TTK-TTT-TSK------------ IrlE_Ddis TPNLESTKKATPT---T-------------TTTT---TTN-S-N-TSAI----------- IrlF_Ddis ------------------------------EKPQ---Q--KQQL-ENQ------------ IrlF_C2d_Ddis ------------------------------EKPQ---Q--KQQL-ENQ------------ 10577_Tthe -------------------------------K---------------------------- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin VAT--------PG----------------RKKPRRG----------------KR-GKKKK AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque -------------------------------------------------------HNLTS AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque IEQ--------PS----------------ITV---S----------------H--RTMTA AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque SQS--------PS----------------TST---V----------------S--HPTTA AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque IPT--------PD----------------SSKT-GS----------------HT-SKTHS AqueK169_Aque -------------------------------------------------------DGIDG IRE-2_Smoe -----------------------------GDRA--S-------------------QENVS IRE-1_Smoe -----------------------------GDRA--S-------------------QENVS IRE2_Hsap RL--Q------------------------SPSK-QA------------------------ IRE1_Hsap KA---------------------------GSSP-SL------------------------ ire-1_Dmel N----------------------------GSNA-NA------------------------ IRE2_Mmus RF--Q------------------------SPSE-PA------------------------ IRE1_Mmus RA---------------------------GTSP-SL------------------------ ire-1_Cele NR---------------------------GSFG-WA------------------------ IRE1_Scer RG--SR-----------------------GGKKGRK------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis --TKKLII-ENNKKQNLINDNNNNNNNNNN------------------------------ IrlB_C2d_Ddis --TKKLII-ENNKKQNLINDNNNNNNNNNN------------------------------ IrlC_Ddis --ITPIKK-EIEKEFEKIELTTPP------------------------------------ IrlD_Ddis --I--------------------------------------------------------- IrlE_Ddis --INEIKNQDLN------------------------------------------------ IrlF_Ddis --IKNLKI-EIKKEEENNKEIKNKKEEVEK------------------------------ IrlF_C2d_Ddis --IKNLKI-EIKKEEENNKEIKNKKEEVEK------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin KDATPIPG-EEGVEGKAPNGPDGPAA---NGTP-NI------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque EK-----------------------------AK-DD------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque AA---------------------------T-SL-ETTQSSGTTVSSYCSSSTDSDPLDAA AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque SA---------------------------T-SP-EA------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque QT---------------------------TDNS-KG------------------------ AqueK169_Aque EM---------------------------QDLK-ST------------------------ IRE-2_Smoe KQ---------------------------VDDA-KV------------------------ IRE-1_Smoe KQ---------------------------VDDT-KV------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque PVMMKVVKFLKPFESQWHLIGVSLELKVDKLVMIDYRNNSSCLATVLNAWKSSPGSVPYT AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap -----------QPLD------------------DPEAEQLTVV-GKISFN--PKDVLGRG IRE1_Hsap -----------EQDD------------------GDEETSVVIV-GKISFC--PKDVLGHG ire-1_Dmel -----------EDL-------------------VDLGNGQVRV-GKISFS--TNEVLGKG IRE2_Mmus -----------QPPH------------------DPEAGQPTVV-GKISFN--PKDVLGRG IRE1_Mmus -----------EQDD------------------EDEETRMVIV-GKISFC--PKDVLGHG ire-1_Cele -----------NRK-------------------IEIPEGWMAVGSKLMYS--PSDILGTG IRE1_Scer -----------SRI-------------------ANIPNFEQSL-K-NLVV--SEKILGYG IreA_Ddis ------------LINSSNKI-----N-NGSGK-IILDNGNVKI-GKLEII--TNKILGTG IrlA_Ddis -----------EQQEQQESIQ----LNQTLTP-I--I-PDFQI-GKFKFSRDENNIIGRG IrlB_Ddis -----------NNNNNNNNN-----NNNKLNN-IIQDEDFVSI-GKFKFNRNESNILGRG IrlB_C2d_Ddis -----------NNNNNNNNN-----NNNKLNN-IIQDEDFVSI-GKFKFNRNESNILGRG IrlC_Ddis -----------------PPL-----A-TTKQQ-QINENYDISI-GKFKFNRKEENVLGRG IrlD_Ddis ----------------------------PTLI-IELNKYDISI-GKFKFSRKDEFIIGRG IrlE_Ddis -------------------Q-----Q-QNITE-NINEIYDVSI-GKFKFNKKESNILGRG IrlF_Ddis -----------EKEENNKEI-----KSKSEFI-IVEDEDFVSI-GKFKFNRNESNILGRG IrlF_C2d_Ddis -----------EKEENNKEI-----KSKSEFI-IVEDEDFVSI-GKFKFNRNESNILGRG 10577_Tthe -----------KNQE-------------------SSEQNANKF-KGLQIN--RNKVLGYG IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin -----------NPPDNNPDATIAPAAPQSPSLVVSSPIIPAAA-GPSLVV--SDTILGFG AqueK159_Aque ------------------------------------------N-RELEVEAEGS------ AqueK160_Aque -----------AAER------------------QPDRHAIKII-GDI--SYICTEKLGDG AqueK161_Aque ----------------------------------------------------KNSLLGKG AqueK162_Aque --------------------------------------LVSIN-ESLSFQYDKKSLLGTG AqueK163_Aque WRSVIEVLEALGDDG------------------NPESSLASIN-ESLSFQYDKKSLLGKG AqueK164_Aque ------------------------------------DQ-DNFT-N--------------- AqueK165_Aque -----------TQPS------------------DPESSLVLIN-ESLSFQFDKKLLLGKG AqueK166_Aque --------------------------------------GLIVN-KSLWYDVCEDSLLGKG AqueK167_Aque -------------------------------------RIKEVN-P--NFSYDVSIKLGQG AqueK168_Aque -----------GGA-------------------IEATD-----G-ERGSN--RSSIALSD AqueK169_Aque -----------EHHF------------------NPGAANEVKI-GAI--TYFRNEKLGDG IRE-2_Smoe -----------DQPK--------------------KQDADGRI-GSLTI---SKIVIGYG IRE-1_Smoe -----------DQPK--------------------KQDADGRI-GSLTI---SKIVIGYG IRE2_Hsap AGGTFVFRGQFEGR--------------------AVAVKRLLRECFGLVRREVQLLQE-- IRE1_Hsap AEGTIVYRGMFDNR--------------------DVAVKRILPECFSFADREVQLLRE-- ire-1_Dmel CEGTFVFKGTFEER--------------------FVAVKRLLPECFTFADREVALLRE-- IRE2_Mmus AGGTFVFRGQFEGR--------------------AVAVKRLLRECFGLVRREVQLLQE-- IRE1_Mmus AEGTIVYKGMFDNR--------------------DVAVKRILPECFSFADREVQLLRE-- ire-1_Cele CEGTVVYRGTFDGR--------------------EVAVKRVVSEFVKFAHREADLLRE-- IRE1_Scer SSGTVVFQGSFQGR--------------------PVAVKRMLIDFCDIALMEIKLLTE-- IreA_Ddis SCGTIVYEGKMEGR--------------------KVAVKRMLSQFVKFADREVSILIH-- IrlA_Ddis SNGTLVFRGIWNDR-------------------IPVAVKQMQKAFNPHISKEIEVLIRLT IrlB_Ddis SNGTLVFKGLWSDK-------------------IPVAIKQMQKAFNPLINKEVEALISLT IrlB_C2d_Ddis SNGTLVFKGLWSDK-------------------IPVAIKQMQKAFNPLINKEVEALISLT IrlC_Ddis SNGTLVFKGIWNNR-------------------IPVAIKQMQKMFNPLISKEIEVLITLT IrlD_Ddis SNGTLVFKGIWNDR-------------------IPVAIKQMHKAFNPLISKEIEVLITLT IrlE_Ddis SNGTLVFKGIWNNR-------------------IPVAIKQMQKMFNPLISKEIEILIGLT IrlF_Ddis SNGTLVFKGIWSDK-------------------IPVAIKQMQKAFNPLINKEVEALITLT IrlF_C2d_Ddis SNGTLVFKGIWSDK-------------------IPVAIKQMQKAFNPLINKEVEALITLT 10577_Tthe AQGTVIFEGTFQGNIQEQKIVFDLINIVYVNIGREIAVKQLLKDNKDLASKEIQMLIKL- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin SHGTVVFQGSLQGR--------------------AVAVKRLLKDFVTLASREVSILQE-- AqueK159_Aque ------------------------------------------------------SL-L-- AqueK160_Aque SKGTTVYKGQFSEH--------------------DVAVKVGKKDV--ITGTEIKMLMQ-- AqueK161_Aque ASST-VYKGTFKRE--------------------GVAVKRMKEE---LSDDDKKFL-L-- AqueK162_Aque ASST-VYKGTFKEE--------------------DVAVKRMKEE---LSDEYKELL-L-- AqueK163_Aque ASST-VHKGTFKEE--------------------AVAVKRMKEE---LSDEYKDLL-K-- AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ASST-VHKGTFKEE--------------------DVAVKRMKEE---LSDEYKELL-L-- AqueK166_Aque KSST-VRRGTYNGQ--------------------EVAVKRLYEKT--LSSEDINLL-L-- AqueK167_Aque ASSI-VHKGTYEGQ--------------------EVAVKKMKDK---LTDDAKLLL-L-- AqueK168_Aque STQSIYRNGSFDSR--------------------PVAVKRVIQNYFDLADREVALLRE-- AqueK169_Aque ASGTTVYKGQFSGK--------------------DVAVKVGKKTA--IRDLEKKIL-E-- IRE-2_Smoe SHGTRILEGKLDQR--------------------QVAVKSILSGFYHKASKEIAALIE-- IRE-1_Smoe SHGTRILEGKLDQR--------------------QVAVKSILSGFYHKASKEIAALIE-- IRE2_Hsap SDRHPNVLRYFCTERG--PQFHYIALELCRASLQEYVEN----------------PDLD- IRE1_Hsap SDEHPNVIRYFCTEKD--RQFQYIAIELCAATLQEYVEQ----------------KDFA- ire-1_Dmel SDAHENVVRYFCTEQD--RQFRYIAVELCAATLQDYTEG----------------DRSL- IRE2_Mmus SDRHPNVLRYFCTEHG--PQFHYIALELCQASLQEYVES----------------PDLD- IRE1_Mmus SDEHPNVIRYFCTEKD--RQFQYIAIELCAATLQEYVEQ----------------KDFA- ire-1_Cele SDTHPHVIRYFCMESD--SQFRYLALELCIASLNDYVEQ----------------KEVQ- IRE1_Scer SDDHPNVIRYYCSETT--DRFLYIALELCNLNLQDLVES----------------KNVS- IreA_Ddis SDEHTNVVRYYAKEED--DEFIYLAISFCQKSLDMYVQQ----------------TLSL- IrlA_Ddis SNNCSNMIRYIDQEED--QLFVYLGLTLCEESLQDLMES----------------KRYK- IrlB_Ddis SKNCSNMIRYIDKEED--KLHVYLGLTLCDGSLQNLVES----------------GKLN- IrlB_C2d_Ddis SKNCSNMIRYIDKEED--KLHVYLGLTLCDGSLQNLVES----------------GKLN- IrlC_Ddis NKNCYNIVRYIDQEED--ESCVYLGLTLCDGSLQNLVEK----------------GDNNL IrlD_Ddis NKNCNNIVRYIDQEED--DMFVYLGLTLCNGSLQNLVEK----------------DLEIN IrlE_Ddis NK-NLNLVGYIDQEED--ENCVYLGLTLCDGSLQSLYDQ----------------SKLN- IrlF_Ddis NKNCSNMIRYIDKEED--KHFVYLGLTLCDVSLQYLVEN----------------GKLN- IrlF_C2d_Ddis NKNCSNMIRYIDKEED--KHFVYLGLTLCDVSLQYLVEN----------------GKLN- 10577_Tthe --QHKNIIKYYYFEET--KDHILLGLEKCVGSISDLIDY----------------ANRK- IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin SDDHPNVIRYYYQEAH--ANFLYIALELCPASLADIIET----------------PDRE- AqueK159_Aque HISHTSPCP--------------------------------------------------- AqueK160_Aque FRKHENIVEYYHSESD--VEYYYLAFQLCLTSLENFIKN----------------EEKR- AqueK161_Aque SIKHDNIVLVHCIY----------------------FKK----------------LKKV- AqueK162_Aque NIKHENIVLVLCIY----------------------FKE----------------FKEV- AqueK163_Aque NIKHENIVLVLCIY----------------------FKE----------------FEKV- AqueK164_Aque ---------------------NIYILEISIST---------------------------- AqueK165_Aque NIKHKNIVLVLCIESY--QGLDHIVLELCLASFHQYFKE----------------FEKV- AqueK166_Aque TIQHENIVKILAIEDG--LENQYIVLELCLASLDLFCVN----------------YKKV- AqueK167_Aque DIKHENIVQLLNIETDEHNEHDYIILELCLASLSKYFKH----------------YETV- AqueK168_Aque SDQHPNVIRYFCMEED--PTFRYLALELCSATLHEYVEG----------------QKMS- AqueK169_Aque RCRHENIVEYYHFDSD--IEYNYLAFQLCDISLENFIEN----------------EDKR- IRE-2_Smoe LDTHPNVVRYYSMEQD--NDFVYIALERCSFNLETFILVHAAGADNRTEVNEFEEKELQ- IRE-1_Smoe LDTHPNVVRYYSMEQD--NDFVYIALERCSFNLETFIVVHAAGADNRTEVNEFEEKELQ- IRE2_Hsap ----------------R----------------------------------GGLEPEVVL IRE1_Hsap ----------------H----------------------------------LGLEPITLL ire-1_Dmel ----------------EL--------------------------------QNHIDVWQVL IRE2_Mmus ----------------R----------------------------------WGLEPTTVL IRE1_Mmus ----------------H----------------------------------LGLEPITLL ire-1_Cele ----------------QN---------------------------------VTIALRDIM IRE1_Scer ----------------DENL------------------------KL----QKEYNPISLL IreA_Ddis ----------------QISPTDSPSIQSSNNNGNGNNGNNNNNNQI----IIDNKTKQMI IrlA_Ddis ----------------EFIEKTT-----------TTNITTTFNNNIIDENLYEQRILSLF IrlB_Ddis ----------------DFVISS------------------------------NKSIIELA IrlB_C2d_Ddis ----------------DFVISS------------------------------NKSIIELA IrlC_Ddis -------------TLTQFLGYDI------------N------SS-----SKNNSRLLELI IrlD_Ddis LISTSNNENNNNNKLKNFI-------------------------------GSELRLLELI IrlE_Ddis ----------------EFINQNN-----------NQ------NN-----NNNNNRVLDLI IrlF_Ddis ----------------EFINSS------------------------------GKSLNELA IrlF_C2d_Ddis ----------------EFINSS------------------------------GKSLNELA 10577_Tthe ----------------NKKKSEQ-DLK---------ALYNIKTD-F----FIKSTMKNTF IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ----------------AFR-------------------------DI----AISFDPKRAL AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ----------------EAILR-----------------------------DFVVSKKEIL AqueK161_Aque ----------------MDKFD-----------------------------GLTVVKKNVN AqueK162_Aque ----------------MNKFN-----------------------------GLTVVKKDVN AqueK163_Aque ----------------TNKFD-----------------------------GLTVVKKDVN AqueK164_Aque ----------------------------------------------------------VF AqueK165_Aque ----------------MNKFN-----------------------------GLTVVKKDVN AqueK166_Aque ----------------ANRFS-----------------------------GFIVVKENII AqueK167_Aque ----------------SSQFV-----------------------------GLNVVIREIL AqueK168_Aque ----------------E----------------------------------FSIDEKHII AqueK169_Aque ----------------KDLIR-----------------------------DFYVNKKEIL IRE-2_Smoe ----------------EILQQKL-----------TL-----WDRSC----LPSPLLMRLM IRE-1_Smoe ----------------EILQQKL-----------TL-----WDRSC----LPSPLLMRLM IRE2_Hsap QQLMSGLAH-LHS-LHIVHRDLKPGNILITGPDSQG------------------------ IRE1_Hsap QQTTSGLAH-LHS-LNIVHRDLKPHNILISMPNAHG------------------------ ire-1_Dmel SQAASGLSH-LHS-LDIVHRDIKPQNVLISLPDAKG------------------------ IRE2_Mmus QQMMSGLAH-LHS-LHIVHRDLKPANILMAGPDSQG------------------------ IRE1_Mmus HQTTSGLAH-LHS-LNIVHRDLKPHNILLSMPNAHG------------------------ ire-1_Cele KQATDGLAH-LHA-SKIVHRDMKPQNVLITMASQRG------------------------ IRE1_Scer RQIASGVAH-LHS-LKIIHRDLKPQNILVSTSSRFTA----------------DQQTGAE IreA_Ddis LELFKGLEH-LHS-LNIVHRDIKPHNVLIDP----------------------------- IrlA_Ddis KDVINGINF-LHC-QDIVHNDLNPRNILVHK----------------------------- IrlB_Ddis KDILFGIQF-LHS-HDIVHNDLNPRNILTLIGKTSN-------------------NNNSS IrlB_C2d_Ddis KDILFGIQF-LHS-HDIVHNDLNPRNILTLIGKTSN-------------------NNNSS IrlC_Ddis KDIVYGIQF-LHQ-QGIVHNDLNPRNILIK------------------------------ IrlD_Ddis KDIVYGIQF-LHQ-QGIVHNDLNPRNILIK------------------------------ IrlE_Ddis IGMINGVIF-LHD-QNIVHNDLNPRNILVK------------------------------ IrlF_Ddis KDIINGVQF-LHS-HDIVHNDLNPRNILTLSTNKNNNNNNKSNNKIKSNNNSNNNSNNNS IrlF_C2d_Ddis KDIINGVQF-LHS-HDIVHNDLNPRNILTLSTNKNNNNNNKSNNKIKSNNNSNNNSNNNS 10577_Tthe KDCLEGLSY-LHS-QGVIHRDIKPENILIHY----------------------------- IRE1_Spur ----------------SVHRDIKPHNVLISQPNQHG------------------------ CC1G_06931_Ccin KQITSGLKH-LHA-LKLVHRDIKPQNILITTNKSTG--------------------RGRP AqueK159_Aque CAKIMATSYPSSS-TRTIHRDIKPGNILLVQKSS------------------------SS AqueK160_Aque HGAIRGLNH-LHNEHNIVHRDVKPSNILIVQRNSG-----------------------SM AqueK161_Aque QDAVKGLEY-LHE-QNIVHRDIKPGNILLVQKSS------------------------SS AqueK162_Aque QDAVRGLEY-LHE-QNIVHRDIKPGNILLVQKSP------------------------LS AqueK163_Aque QDAVRGLEY-LHE-QSIVHRDIKPGNILLVQKSS------------------------SS AqueK164_Aque WQCIKKPEA-I-D-QWPVGRNRHAGAIIITG--SDCPML--------------------- AqueK165_Aque QDAVRGLEY-LHE-QNIVHRDIKPGNILLVQKSS------------------------SS AqueK166_Aque RDAVKGLEY-LHK-RWIVHRDIKPGNILLLQKGS------------------------SS AqueK167_Aque QDAVKGMEY-LHK-RNVVHRDLKPGNILLVQKSSS-----------------------SA AqueK168_Aque AESMSGINH-LHS-LGIVHRDLKPQNVLLRTS-SSL------------------------ AqueK169_Aque FDSIKGLSY-LHK-ENIVHRDVKPSNILLVRRNSS-----------------------SM IRE-2_Smoe RDVLEGLVH-LHK-LGYVHRDLKPQNVLILRSGT-------------------------- IRE-1_Smoe RDVLEGLVH-LHK-LGYVHRDLKPQNVLILRSGT-------------------------- : .: :. :: IRE2_Hsap LGRVVLSDFGLCKKLPAGRCSFSL--HSG-IPGTEGWMAPELLQL-LPP----------- IRE1_Hsap KIKAMISDFGLCKKLAVGRHSFSR--RSG-VPGTEGWIAPEMLSE-DCK----------- ire-1_Dmel KVRVMISDFGLCKKLNFGKTSFSR--RSG-VTGTDGWIAPEMMRS-QRTVRHDFYYYPTL IRE2_Mmus QGRVVISDFGLCKKLPVGRCSFSL--HSG-IPGTEGWMAPELLQL-P-P----------- IRE1_Mmus RIKAMISDFGLCKKLAVGRHSFSR--RSG-VPGTEGWIAPEMLSE-DCK----------- ire-1_Cele EMRAVISDFGLCKRVQPGKNSISRGIASG-LAGTDGWIAPEVLIS-A------------- IRE1_Scer NLRILISDFGLCKKLDSGQSSFRTNL-NN-PSGTSGWRAPELLEE-SNNLQCQVE----- IreA_Ddis NNRVKISDMGLGKLLDNDDQSLTF--T----SDSHGWQPAEYLNG-T------------- IrlA_Ddis -GNFVISDLGLSKMQVETSYSFTN--N-A-PTGQEGYHPIEVLQE--------------- IrlB_Ddis NNSFIISDLGLSKMEVESSYSFTS--N-I-PTGQGGYHPFEVLQS--------------- IrlB_C2d_Ddis NNSFIISDLGLSKMEVESSYSFTS--N-I-PTGQGGYHPFEVLQS--------------- IrlC_Ddis DDRFIISDLGLSKMEVTSSYSFTM--H-A-PTGQEGFHPAEVLLE--------------- IrlD_Ddis DDRFIISDLGLSKMEVTSSYSFTM--H-A-PTGQEGFHPAEVLLE--------------- IrlE_Ddis DNRLIISDLGLSKMNVSSTYNFST--NAI-PTGQDGYHPVEVLLE--------------- IrlF_Ddis NNSFIISDLGLSKMEVESSYSFTT--N-V-PTGQGGYHPVEVLQS--------------- IrlF_C2d_Ddis NNSFIISDLGLSKMEVESSYSFTT--N-V-PTGQGGYHPVEVLQS--------------- 10577_Tthe NKEIKLADFGLSKNISKTNVIFTN--D----IGTWGWRPLEQIDN--------------- IRE1_Spur KVKAMISDFGLCKKLSAGRMSFSR--RSG-VAGTDGWIAPEMLTG-Q------------- CC1G_06931_Ccin TYRMLISDFGLCKKLDVDQTSFLPTMNGGMGAGTVGWRAPEILRG-EVKLDDL------- AqueK159_Aque EVKAVLADFGISTELNEREAV-TR--Q-R-GRRTDTWMAPELLLD-DLT----------- AqueK160_Aque QIKAVISDFGIATELEPDRYSQTT--I-G-LKGTLMWLAPELVNK-TV------------ AqueK161_Aque EVKAVLADFGISTKLNNRDAV-TT--Q-R-RRGTDTWMGPELLLD-DIK----------- AqueK162_Aque EVKAVLADFDLSAKLDDDRSSKTT--L-N-KRGTLSWMAPELLVE-DGL----------- AqueK163_Aque EVKAVLADFGLSTKLDDDQFSKTT--V-D-GRGTKAYIPPELLVE-DGS----------- AqueK164_Aque ---VISGGFDKNIDTLDDCWIFN--------ITQHSWIKLDVPHSVS------------- AqueK165_Aque EVKAVLADFDLSTKLDDDRSLKTT--L-N-KRGTLSWMAPELLVK-DGL----------- AqueK166_Aque EVKAVLADFGISTKLTSRDSK-TT--H-R-GRVTDTWMAPELLLD-DPS----------- AqueK167_Aque DVKAVLADFGISVTLIDDLNSLTT--R-T-ARGTDTWKAPELLLD-NRK----------- AqueK168_Aque KVRALISDFGLCRKLPDGRGSFTA--QSG-ILGTEGWIAPEMFQD-R------------- AqueK169_Aque KIKAVISDFGIAAELDPGRISKTT--T-G-LKGTLLWVAPELLNK-RRDP---------- IRE-2_Smoe FLSAKISDMGISKRLNDGASGIDK--LTT-GMGSSGWQAPEQIQG-E------------- IRE-1_Smoe FLSAKISDMGISKRLNDGASGIDK--LTT-GMGSSGWQAPEQIQG-E------------- ..:. : : IRE2_Hsap ------------------------------------------------------DSPT-- IRE1_Hsap ------------------------------------------------------ENPT-- ire-1_Dmel LLHHHKNFLK---------------------------------------SMSFNYLQT-- IRE2_Mmus ------------------------------------------------------DSPT-- IRE1_Mmus ------------------------------------------------------DNPT-- ire-1_Cele -------------------------------------------------------STS-- IRE1_Scer ----------TEHSSSRHTVVSSDS---------------------FYDP-FTKRRLT-- IreA_Ddis ------------------------------------------------------NRNT-- IrlA_Ddis ------------------------------------------------------KRKT-- IrlB_Ddis ------------------------------------------------------KRMT-- IrlB_C2d_Ddis ------------------------------------------------------KRMT-- IrlC_Ddis ------------------------------------------------------KRKT-- IrlD_Ddis ------------------------------------------------------KRKT-- IrlE_Ddis ------------------------------------------------------KRKT-- IrlF_Ddis ------------------------------------------------------KRMT-- IrlF_C2d_Ddis ------------------------------------------------------KRMT-- 10577_Tthe ------------------------------------------------------QPLT-- IRE1_Spur ------------------------------------------------------DRTT-- CC1G_06931_Ccin ---------SD------------DHSMSSRGSVSTINGSSSSSSTSGLST-AKPTRLT-- AqueK159_Aque -----------------------------------------------------DCKFS-- AqueK160_Aque ------------------------------------------------------KKFS-- AqueK161_Aque -----------------------------------------------------ACKFS-- AqueK162_Aque -----------------------------------------------------ELQFS-- AqueK163_Aque -----------------------------------------------------KLKFS-- AqueK164_Aque ------------------------------------------------------KRRSHS AqueK165_Aque -----------------------------------------------------ELQFS-- AqueK166_Aque -----------------------------------------------------GCKFS-- AqueK167_Aque -----------------------------------------------------QRKCG-- AqueK168_Aque ------------------------------------------------------TRVT-- AqueK169_Aque -----------------------------------------------------RLKYS-- IRE-2_Smoe -------------------------------------------------------RQT-- IRE-1_Smoe -------------------------------------------------------RQT-- IRE2_Hsap SAVDIFSAGCVFYYVLSGGSHPFGD-SLYRQA----NILTG-APCLAH-LEEEV----HD IRE1_Hsap YTVDIFSAGCVFYYVISEGSHPFGK-SLQRQA----NILLG-ACSLDC-LHPEK----HE ire-1_Dmel TAVDIFSLGCVYYYVLSGGHHAFGD-NLKRQA----NILSH-EYNLAK-LRPEDDSEDSR IRE2_Mmus SAVDIFSAGCVFYYVLSGGSHPFGE-SLYRQA----NILSG-DPCLAQ-LQEET----HD IRE1_Mmus YTVDIFSAGCVFYYVISEGNHPFGK-SLQRQA----NILLG-ACNLDC-FHSDK----HE ire-1_Cele YPVDIFSLGCIFYYVLTSGTHPFGK-SLHRQA----NIVNG-EYTLNK-LAD-L----DD IRE1_Scer RSIDIFSMGCVFYYILSKGKHPFGD-KYSRES----NIIRG-IFSLDE-MKCLHDR--SL IreA_Ddis KKVDIFSLGCVVYYLLT-GAHPFGH-RYNREK----NVLKG-KFDIDQ-IK--------H IrlA_Ddis KSVDIFSLGCILFYLLTNGQHPFGNNKLLRVA----NIVYD-KPDLEP-LK--------F IrlB_Ddis KSVDIFSLGCILFYLLTNGQHPFGNDKLFRIV----NIISN-KMNLTP-LN--------S IrlB_C2d_Ddis KSVDIFSLGCILFYLLTNGQHPFGNDKLFRIV----NIISN-KMNLTP-LN--------S IrlC_Ddis KSVDIFSMGCILFYLMTGGQHPFGD-KFYRIV----NILTD-KPILEP-LK--------H IrlD_Ddis KSVDIFSMGCILFYLMTGGQHPFGD-KFFRMA----NILTD-KPILEP-LK--------H IrlE_Ddis KSVDVFSLGCLIYFIMTNGAHPFGD-KFSRLR----YITKS-KYNLSQ-LS-------NL IrlF_Ddis KSVDIFSLGCILFYLFTNGQHPFGNDKLFRIY----NIMLN-KVNLEL-LG--------H IrlF_C2d_Ddis KSVDIFSLGCILFYLFTNGQHPFGNDKLFRIY----NIMLN-KVNLEL-LG--------H 10577_Tthe YNTDVFSLGCVFYYIYTQGGHPFGE-YLRM-----------------------------E IRE1_Spur TAIDIFSLGCVFYYVLSNGKHPFGD-SLHRQA----NIISG-EYSLDL-LP-------PD CC1G_06931_Ccin KSVDIFALGCLYYYTLTNGSHPYGD-RFEREV----NILKD-AKSLDL-LERFG----EE AqueK159_Aque RESDIFSMGCIIYYVLTKGRHPFNK-GHDNDYTVHQNIKNYMICDFQ-----LL----ND AqueK160_Aque KAVDIFSMGCVMYYVLSDGHHPFGN-DIV---AIIQNFGTH-SYTLDD-ISDEV----KD AqueK161_Aque RESDIFSMGCVIYFVETGGSHPFDK-GHDDDYTIHQNVKNYDRCDFR-----LL----HG AqueK162_Aque LQSDIFSMGCVIYFAETDGNHPFDT-GDSRGAAIHLNVKNYNPCDFH-----LL----NG AqueK163_Aque PQSDIFSMGCVIYFAETDGHHPFDT-GDSRGAAIHQNVKDYNPCDFR-----LL----DG AqueK164_Aque LSVFIMSPHCV--WIITAGGAVMTET-LVTNP----NIVML-TELVTDSKG--------E AqueK165_Aque LQSDIFSMGCVIYFAETDGNHPFDT-GDSRGAAIHQNVKNYNPCDFH-----PL----NG AqueK166_Aque RQSDIFSMGCVTYYLITKGHHPFNR-GHDNVFRIHQNVKNYVTCDFQ-----LL----ND AqueK167_Aque RESDIFAMGCVIYFAETGGNHPFDT-GDNA--DIDQNVKMYKKCDFS-----LL----DD AqueK168_Aque CAVDIFAMGCVIYYVLSCGGHPFGP-PLKRQA----NIEAG-DFSLKA-LIG------ED AqueK169_Aque KAVDVFSMGCVMYYVLSDGRHPFGE-DII---TIVQNFGTH-SYRLDG-ISGKV----ED IRE-2_Smoe RAVDMFSVGCLLYFCITGGQHPFGG-RLERDM----NIVSG-KMDLFA-V--------DH IRE-1_Smoe RAVDMFSVGCLLYFCVTGGQHPFGG-RLERDM----NIVSG-KMDLFA-V--------DH ::: *: : : * IRE2_Hsap KVVARDLVGAMLSPLPQPRPSAPQVLAHPFFWSRAKQL-QF-------FQD-VSDWLEKE IRE1_Hsap DVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQL-QF-------FQD-VSDRIEKE ire-1_Dmel IILAEQLISDMIHKDPQSRPPARCIGNHPLFWDEPKML-SF-------LQD-VSDRVEKL IRE2_Mmus KVVALDLVRAMLSLLPQDRPSAGWVLAHPLFWSRAKEL-QF-------FQD-VSDWLEKE IRE1_Mmus DVIARELIEKMIAMDPQQRPSAKHVLKHPFFWSLEKQL-QF-------FQD-VSDRIEKE ire-1_Cele WSLADDLISSMLNVEPLHRLTADAVLNHPFFWTSEKRL-AY-------FSD-VSDRVEKE IRE1_Scer IAEATDLISQMIDHDPLKRPTAMKVLRHPLFWPKSKKL-EF-------LLK-VSDRLEIE IreA_Ddis LPDIHQLVHSMIQFEPEKRPDIGECINHPFFWEVHKKL-SF-------LVA-ASDYLEFE IrlA_Ddis NAPALDLVRLMISQDEKKRPTIDTILNHPLFWSTNEKI-KF-------YES-SLNLLK-D IrlB_Ddis NQLACTLIKSMISKDESIRPTIQNVLNHPLFWNLEKKI-QF-------IDA-ALNLIK-E IrlB_C2d_Ddis NQLACTLIKSMISKDESIRPTIQNVLNHPLFWNLEKKI-QF-------IDA-ALNLIK-E IrlC_Ddis NLVACDLISQMISKNESDRPTIEKILLHPFFWNHEKKV-KF-------IDA-SLNLFK-D IrlD_Ddis NLVACDLISQMISKNESDRPTTENILLHPFFWNHEKKV-KF-------IDA-SLNLFK-D IrlE_Ddis NLVATHLIELMISYDESKRPTLSSVLKHPLFWDSLKKI-KF-------LES-SLRLLG-D IrlF_Ddis NLLACDLIKSMISNDESKRPTIENVLNHPLFWNVEKKI-QF-------IDA-ALNICK-E IrlF_C2d_Ddis NLLACDLIKSMISNDESKRPTIENVLNHPLFWNVEKKI-QF-------IDA-ALNICK-E 10577_Tthe DETLKDLIQKMIHNDQSKRLTVKECLKHPYFWNTLQKF-SF-------ICE-FSDYIETF IRE1_Spur DEIAHQLITQMVDPYFDTRPEANAILRHPFFWYPEKQL-AF-------FQD-VSDRIEKE CC1G_06931_Ccin GTEACDLIEKMLDPEASERPDTTACLLHPFFWDPARRL-NF-------LQD-ASDRFEIM AqueK159_Aque KPVARALIKNMMKHNKDSRPSASEVLDDPYFKEDDSQD-TIL-----LSPD-K-E----- AqueK160_Aque RETAKHLIKQMIHKDKDLRISVEEVLNHCYFWSNERQL-DF-------LEA-ASDILEFM AqueK161_Aque KPKATALIKKTIQLDRASRPSASEVLDDPYFQEDDPQD-TIH-----VPP-QLTEGEEAP AqueK162_Aque KPKATALILKMIKRDRASRPSASEVLDDPYFKEDDSQD-TIQ-----VLPQ-LTDGEEAP AqueK163_Aque KPIASALINKMIQHNRASRPSASEVLQDPYFKEDDPQD-TVH-----VPPQ-VTEGEEAP AqueK164_Aque WTVGDTLDTNGMNNEEYKKKYQQQLQTGRRIWLEEYQK-PR-------KGD-TADIEQTV AqueK165_Aque KPKATALIFKMITRDRASRPSASEVLDDRYFKEDDPQD-TVQ-----VPQPQLTEGEEAP AqueK166_Aque KPLARALIKNMIKYNKDSRPCASEVLGDPYFKEDGPQD-IIQ-----VPQD-S-E----- AqueK167_Aque KPKAKDLIEKMIQLDKYSRPTASVVLQDPYFEEDGHVPCEYKKKSPSTFPD-TSQTNIAP AqueK168_Aque RYTAEHLVNNMISFNSKLRPTADEVLHHCLFWSKSRQM-SF-------FQD-VSDRIEKE AqueK169_Aque REPAKDLIVQMIHENKDLSVV--------------------------------SDIYK-- IRE-2_Smoe YPEAIDIISSLLAMDPKDRPTSMQVKLHPFFWPPQQRL-QF-------LIT-ASDRVERE IRE-1_Smoe YPEAIDIISSLLAMDPKDRPTAMQVKLHPFFWPPQQRL-QF-------LIT-ASDRVERE : : IRE2_Hsap SE--QE--------------------------------------PLVR------------ IRE1_Hsap SL--DG--------------------------------------PIVK------------ ire-1_Dmel QF--HA--------------------------------------EPLK------------ IRE2_Mmus PD--QG--------------------------------------PLVS------------ IRE1_Mmus AL--DG--------------------------------------PIVR------------ ire-1_Cele ED--NS--------------------------------------PVVR------------ IRE1_Scer NRDPPS--------------------------------------ALLM------------ IreA_Ddis KPTSP----------------------------------------LNL------------ IrlA_Ddis PNNSQS--------------------------------------KHSKLLNY-------- IrlB_Ddis PSNSSYN------------------------------------SKLTKQL---------- IrlB_C2d_Ddis PSNSSYN------------------------------------SKLTKQL---------- IrlC_Ddis SNGL-FT------------------------------------SKLNKLINYQEINLK-- IrlD_Ddis SNGL-FT------------------------------------SKLNKLINQFQD----- IrlE_Ddis HDFK--K------------------------------------FNINKILISCNSNSSSS IrlF_Ddis SNNSGGG---------------------GGG------------NKFNKSLNYLF------ IrlF_C2d_Ddis SNNSGGG---------------------GGG------------NKFNKSLNYLF------ 10577_Tthe DTENKY----------------------------------------SDLI---------- IRE1_Spur PL--DC--------------------------------------PLLV------------ CC1G_06931_Ccin CRDPKD--------------------------------------PLLL------------ AqueK159_Aque ------------------------------------PKQNSLDNLYDKQLYHVMV----- AqueK160_Aque P--STKD-HMIEH----------------------------------------------- AqueK161_Aque QGATLQDTSEVQDGASIPP-----------------PEQSVTDTSYGKQLHNSMS----- AqueK162_Aque QVDTPQDTSEVQDGASILLPEESVS----------------------------------- AqueK163_Aque QVDTPQDTSEVQDEASILPPEQSVSADGEGHVSIPSPEQSVTDTSYGEQLYAVMS----- AqueK164_Aque -------------------------------------------QALMK------------ AqueK165_Aque QGATPQDTIEVQDEASILPPEQSVSANGEGHTSIPSPEQSVTDTSYGKQLYDVML----- AqueK166_Aque ------------------------------------PDQN---KVYGKQLYHAMV----- AqueK167_Aque IIPTPDGT----NTAVTLTPANPDSS-------------------------NATV----- AqueK168_Aque TP--QS--------------------------------------AVVQ------------ AqueK169_Aque ----------TQT----------------------------------------------- IRE-2_Smoe DREASS--------------------------------------AVLT------------ IRE-1_Smoe DREASS--------------------------------------AVLT------------ IRE2_Hsap -----------------------------------------ALEAGGCAVV--RDNWHE- IRE1_Hsap -----------------------------------------QLERGGRAVV--KMDWRE- ire-1_Dmel -----------------------------------------SLEKNGRIVV--LDDWNV- IRE2_Mmus -----------------------------------------ALEAGSYKVV--REDWHK- IRE1_Mmus -----------------------------------------QLERGGRAVV--KMDWRE- ire-1_Cele -----------------------------------------RIETDARIVV--CGGWRE- IRE1_Scer -----------------------------------------KFDAGSDFVIP-SGDWTV- IreA_Ddis -----------------------------------------EIDSHVDLVTDGSGDWWL- IrlA_Ddis --------------------------YQ-------------NDNSGGVLFL--SKPWNQ- IrlB_Ddis ---------------------------N-------------HCDDNDEPFL--NDSWNH- IrlB_C2d_Ddis ---------------------------N-------------HCDDNDEPFL--NDSWNH- IrlC_Ddis NNI-DSSSSNNNNNINSNVIN-NNNNNN-------------NGMATNIPFL--SKPWNQ- IrlD_Ddis --------------------------TD-------------GVNTTSTPFL--SKPWNQ- IrlE_Ddis NSICNSS---NSSSSSSSSISSS---SC-------------KISSSSCYCV--PLPWNQ- IrlF_Ddis ------------------VISLS---SD-------------YIEPKSEPFL--KQTWDK- IrlF_C2d_Ddis ------------------VISLS---SD-------------YIEPKSEPFL--KQTWDK- 10577_Tthe ---------------------------E-------------Q-QAKSLNVLE-GNRWDK- IRE1_Spur -----------------------------------------ALETGAQNVV--RGDWRN- CC1G_06931_Ccin -----------------------------------------QLETGALAIV--GNDWHA- AqueK159_Aque ---------------------NI---SRSLPEFIVDHYMRLMAVVGVKSGI--EPDWSLS AqueK160_Aque ---------------------------------------------GAADVI--GTNWMN- AqueK161_Aque ---------------------DM---SHSLPRYIVDQYIRLLTIVGVKCDM--KPDWSLS AqueK162_Aque -----------------------------------------------------EHASHA- AqueK163_Aque ---------------------DM---SHSLPGYIVDQYIRLLAIINVKCDM--KPDWLLS AqueK164_Aque --------------------SL-----E-------------EKEREAQV-------YHQ- AqueK165_Aque ---------------------NM---SHSLPRYIVDQYIRLLAIINVKCDV--KPDWSLS AqueK166_Aque ---------------------SI---SHSVPQYIVDNYTRLLAVVGVKSYI--KPDWSLS AqueK167_Aque ---------------------TI---SDYV------------------------------ AqueK168_Aque -----------------------------------------SLERGANHVI--KGDWRD- AqueK169_Aque ---------------------------------------------GT------------- IRE-2_Smoe -----------------------------------------ALEAVAPTAL--GGAWDE- IRE-1_Smoe -----------------------------------------ALEAVAPTAL--GGAWDE- IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque RRFLELYSTHASKKSPQVAA---------------------SYVDKILTGLGYEEMGEFS AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque KKFLELYSTHTSEKSPQVAA---------------------SYVYKILTGLGYEEMGDFR AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque KKFLELYSTHVSNTSPQVAA---------------------SYVYKILTGLGYEEMDDFS AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque RRFLELYSTHASEKSPQVAA---------------------SYVYKILTGLGYEEMEDFR AqueK166_Aque KRFLELYSTHASQTSPQVAA---------------------SYVDKILTGLGYEEIKSFK AqueK167_Aque -----TTSTITAEEAPQASQVDGDEASKPSKHQNEASSITESGLEEFIDGQVVDEIKTDS AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ----------------------------------HISMPLQTDL---------------- IRE1_Hsap ----------------------------------NITVPLQTDL---------------- ire-1_Dmel ----------------------------------HLDPMITDDL---------------- IRE2_Mmus ----------------------------------HISAPLQADL---------------- IRE1_Mmus ----------------------------------NITVPLQTDL---------------- ire-1_Cele ----------------------------------KICDALKEDL---------------- IRE1_Scer ----------------------------------KFDKTFMDNL---------------- IreA_Ddis ----------------------------------KIDQVLIDNI---------------- IrlA_Ddis ----------------------------------IIDPFLIDHV------ENNNNN---- IrlB_Ddis ----------------------------------LIDVTNLL------------------ IrlB_C2d_Ddis ----------------------------------LIDVTNLL------------------ IrlC_Ddis ----------------------------------LIDQTLIEHI------INKQNQLN-- IrlD_Ddis ----------------------------------LIDPTLIEHI------TNKQNQLSGG IrlE_Ddis ----------------------------------SLDYQLVDSL------SN-------- IrlF_Ddis ----------------------------------LIDINNL------------------- IrlF_C2d_Ddis ----------------------------------LIDINNL------------------- 10577_Tthe ----------------------------------NVDKSLIQDT---------------- IRE1_Spur ----------------------------------NITEELQTDL---------------- CC1G_06931_Ccin ----------------------------------RLDRLFVENL---------------- AqueK159_Aque VY-------------RY--LHTE----------VDIDSKVNEKL------E--------- AqueK160_Aque ----------------------------------QIDA---T--------D--------- AqueK161_Aque AY-----------------LHTE----------VEIDSKITEEL------E--------- AqueK162_Aque ----------------------------------RIHSILLPEE------S--------- AqueK163_Aque AY-----------------LHTE----------VEIDTKINEEL------E--------- AqueK164_Aque ----------------------------------QLEQKEREEAKKDQKIRRYRHQ---- AqueK165_Aque AY-----------------LHTE----------VEIDTKINEEL------E--------- AqueK166_Aque AY-----------------LRFD----------VDIDSKVTEKL------K--------- AqueK167_Aque DLVYNALNNGVKKSVFLDEIKVATQEKAITFQKLENDIALTKDI------K--------- AqueK168_Aque ----------------------------------HIGEELRQDL---------------- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ----------------------------------KLDPLLINNL---------------- IRE-1_Smoe ----------------------------------KLDPLLINNL---------------- IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ----NN--------------------------NNNNN----------------------- IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------G----------------------- IrlD_Ddis SSIGNN--------------------------NNNSL----------------------- IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ----LQEKDREHQVVLQENQEALQQKDRKHQVVLQELQEKDREHQVVLQEKDREHQVVLQ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ----------------RKFRSYKGTSVRD-------------------LLRA-VRNKKHH IRE1_Hsap ----------------RKFRTYKGGSVRD-------------------LLRA-MRNKKHH ire-1_Dmel ----------------RKYRGYMGASVRD-------------------LLRA-LRNKKHH IRE2_Mmus ----------------KRFRSYKGTSVRD-------------------LLRA-MRNKKHH IRE1_Mmus ----------------RKFRTYKGGSVRD-------------------LLRA-MRNKKHH ire-1_Cele ----------------RKFRTYKSFSVRD-------------------LLRA-MRNKKHH IRE1_Scer ----------------ERYRKYHSSKLMD-------------------LLRA-LRNKYHH IreA_Ddis ----------------GRYRKYNGKSIRD-------------------LLRV-IRNKFNH IrlA_Ddis ----KQ------NSKKLAIVAYQYDQVRD-------------------LVRC-IRNSLVH IrlB_Ddis ----TP------TKGSKITISYQYDKVRD-------------------LIRF-IRNTIAH IrlB_C2d_Ddis ----TP------TKGSKITISYQYDKVRD-------------------LIRF-IRNTIAH IrlC_Ddis ----VG------N--NKKVIIYSFDQVKD-------------------LVRC-IRNTIQH IrlD_Ddis ----TL------S--GKKFYFYDYSQVKD-------------------LVRC-IRNTIQH IrlE_Ddis -----Q------I--EKKVASYKFDQLHD-------------------LIRF-IRNTLQH IrlF_Ddis ------------S--IGSTSQYQYDQIKD-------------------LIRF-IRNTIVH IrlF_C2d_Ddis ------------S--IGSTSQYQYDQIKD-------------------LIRF-IRNTIVH 10577_Tthe ----------------KVFKFYNYGQVKD-------------------LIRL-IRNRKSH IRE1_Spur ----------------RKFRAYRGHSVRD-------------------LLRA-MRNKKHH CC1G_06931_Ccin ----------------GKFRKYDGKSVQD-------------------LLRA-LRNKKHH AqueK159_Aque --------------KIMKFRPAVIQVVRE-LCLCLHDYQVERYRMVVTLLRRTALDSNKS AqueK160_Aque ---------------I--PGNYDPTLVKD-------------------LLRA-IKAKKHS AqueK161_Aque --------------KITEFRPAIIKVVRDELGPRLGDYQIDRYRMVLTVLCSITFDSNKS AqueK162_Aque --------------VSAHA----------------------SHASIPSPGQS-VSADGED AqueK163_Aque --------------KITEFRPAVIKVIRGELGPRLGDYQIDRYRMVLTVLCITTFDSNKS AqueK164_Aque EKDREL------RQSQEAVRRYQQQALTD-------------------DHWV-IN-KD-- AqueK165_Aque --------------KITEFRPAVIKVIRDELGPRLGDYQIDRYRMVLTVLCSIAFDSNKS AqueK166_Aque --------------ELKEFRPAVIQVVYA-LGLCLNSHQVERYRMVVTVLRGTALDANKS AqueK167_Aque -----DVYAASQFIKAAKFDAYNSTIETD-------------------LVPA-PIPPSAL AqueK168_Aque ----------------RRFRSYQGTSLRD-------------------LLRA-MRNKKHH AqueK169_Aque --------------------------------------------------KF-ILNTDHK IRE-2_Smoe ----------------GRYRQYNYQAVRD-------------------LLRV-IRNKANH IRE-1_Smoe ----------------GRYRQYNYQAVRD-------------------LLRV-IRNKANH IRE2_Hsap YRELPVEVRQALG----------------------------------------------- IRE1_Hsap YRELPAEVRETLG----------------------------------------------- ire-1_Dmel YHELTPAAQKMLG----------------------------------------------- IRE2_Mmus YRELPAEVRQTLG----------------------------------------------- IRE1_Mmus YRELPVEVQETLG----------------------------------------------- ire-1_Cele YRELPEDVRQSLG----------------------------------------------- IRE1_Scer FMDLPEDIAELMG----------------------------------------------- IreA_Ddis YRDLSPEEQTCLG----------------------------------------------- IrlA_Ddis HKDILRSITQQQN-LPPSSK---------------------------------------- IrlB_Ddis HKEIKRAIIQQFQNQQSRPN---------------------------------------- IrlB_C2d_Ddis HKEIKRAIIQQFQNQQSRPN---------------------------------------- IrlC_Ddis HKEIQRLVRQSPS-SNGDNK---------------------------------------- IrlD_Ddis HKEIQRLISQSPS-SS--NK---------------------------------------- IrlE_Ddis YNQIYRDLKQILP-NS-------------------------------------------- IrlF_Ddis HKDIKRLIQQQQQ-QQKQPT---------------------------------------- IrlF_C2d_Ddis HKDIKRLIQQQQQ-QQKQPT---------------------------------------- 10577_Tthe YHELSESSKDIVG----------------------------------------------- IRE1_Spur FRELPEEVKASLG----------------------------------------------- CC1G_06931_Ccin YQDLPDNVKRSLG----------------------------------------------- AqueK159_Aque FLELFMDLTQELG-KYPLAVAITLGVLEKSDWGDTGKLKPFSLPNFDLNTSYPEVDLCLT AqueK160_Aque AGIVSVSS---------------------------------------------------- AqueK161_Aque FFELFTDLTGNLG-KYPLAVSLTIGVLERSGWGDTKKLKLFSLPNFDINTSYPKIYLCLT AqueK162_Aque YTSIPSPE-QSLS-ADG------------------------------------------- AqueK163_Aque FFELFTDLTGNLG-KYPLAVSLTIGVLERSGWGDTKKLKPFLLPNFDLNTSYPKIDLCLT AqueK164_Aque --EV-TLTKEELG----------------------------------------------- AqueK165_Aque FFELFADLTGNLG-KYPLAVSLTIGVLERSGWGDTKKLKPFSLPNFDLNTSYPKIDLCLT AqueK166_Aque FIRLFTDLMEELG-KYPLAVAITVGVLERSDWGDTRKLKPFSLPNFDINTSYPKIDLCLT AqueK167_Aque FPELSN--IQCVS-ELPDHL---------------------------------------- AqueK168_Aque YRELPETLKESLG----------------------------------------------- AqueK169_Aque AGDQQET----------------------------------------------------- IRE-2_Smoe YRELPADVQQILG----------------------------------------------- IRE-1_Smoe YRELPADVQQILG----------------------------------------------- IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque VAGYYGYMRDLDFSNAKVYTSSLHLKSHNVSNMSRVQFTRLLMERGVLEAGDVSKLEDKD AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque VADYYGNMSQRDFSSAKVYTSSLHLNSHNVSNMSRVQFTLLLMERGVIEVGDVSKIEDND AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque VADYYGNMSQRDFSSAKVYTSSLHLNSHNVSNMSRVQFTLLLMERSVIEAGNVSKIEDKV AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque VADYYGNMSQRDFSSAKVYTSSLHLKSHNVSNMSRVQFTRLLMERSVVEAGNVSNIEDTV AqueK166_Aque VADYYGKMSDIDFSSAKVCTSVVHLKSHNVSNMSRVQFTLLLMERGVIEAGDVSKIEDKI AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque RYPLFFKKYKKRCKGKEL------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque RYPLFFKKYKQRCKGPSKVTAPATDET----------VAVQQRKAQATSGQSPSTSTDQP AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque RYPIFFKEYEKRCKGPTKVTAPATNETVVPSLP------IQEMKAQATSSQSPSTPTDQP AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque RYPTFFEKYEKRCKGPSKETAPMTNETVVPSLPTQETVPIQEIKAQATLGQSPSAPTDQP AqueK166_Aque RYPIFFEKYKQRCKGPSKET---------------------------------------- AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ----------------------------------------------------QVPD---- IRE1_Hsap ----------------------------------------------------SLPD---- ire-1_Dmel ----------------------------------------------------CIPH---- IRE2_Mmus ----------------------------------------------------QLPA---- IRE1_Mmus ----------------------------------------------------SIPD---- ire-1_Cele ----------------------------------------------------DIPD---- IRE1_Scer ----------------------------------------------------PVPD---- IreA_Ddis ----------------------------------------------------ILPD---- IrlA_Ddis --------------------------------------------EFANDCL-KSQE---- IrlB_Ddis -----------------------------------------LE---VLEYL-SSQD---- IrlB_C2d_Ddis -----------------------------------------LE---VLEYL-SSQD---- IrlC_Ddis -----------------------------------------QE---VLDCL-ESQE---- IrlD_Ddis -----------------------------------------QE---VLDCL-ESQE---- IrlE_Ddis ------------------------------------------D---ILESL-KSQQ---- IrlF_Ddis -----------------------------------------IELQFINNIL-TNQD---- IrlF_C2d_Ddis -----------------------------------------IELQFINNIL-TNQD---- 10577_Tthe ----------------------------------------------------DTLD---- IRE1_Spur ----------------------------------------------------KVPD---- CC1G_06931_Ccin ----------------------------------------------------PMPE---- AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque SFLTQSRKHKISSNYSGILPCVGGRGSPTGDVHIYVKRILPES---VLQKDSK------- AqueK162_Aque ---------------ED-------------------YISIPPE---QSKQLYAV------ AqueK163_Aque NIFTRVKNFFLPSSKQQ-------------------AQEVPGT---TTQAKEPTPESSNT AqueK164_Aque ----------------------------------------------------RGSY---- AqueK165_Aque NFFTRVKKFFLPSSKQE-------------------AQEVPGT---TTQAKEPTPESSNT AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ----------------------------------------------------QIPD---- AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ----------------------------------------------------SIPQ---- IRE-1_Smoe ----------------------------------------------------SIPQ---- IRE2_Hsap -----------------------------------------------------------G IRE1_Hsap -----------------------------------------------------------D ire-1_Dmel -----------------------------------------------------------E IRE2_Mmus -----------------------------------------------------------G IRE1_Mmus -----------------------------------------------------------D ire-1_Cele -----------------------------------------------------------Q IRE1_Scer -----------------------------------------------------------G IreA_Ddis -----------------------------------------------------------G IrlA_Ddis -----------------------------------------------------------S IrlB_Ddis -----------------------------------------------------------S IrlB_C2d_Ddis -----------------------------------------------------------S IrlC_Ddis -----------------------------------------------------------L IrlD_Ddis -----------------------------------------------------------L IrlE_Ddis -----------------------------------------------------------S IrlF_Ddis -----------------------------------------------------------S IrlF_C2d_Ddis -----------------------------------------------------------S 10577_Tthe -----------------------------------------------------------D IRE1_Spur -----------------------------------------------------------Q CC1G_06931_Ccin -----------------------------------------------------------G AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque APAPTVTSAAKEGTLPAPAPPIAATVPASTPPTAPIVSPPISPTATVPAPVPLPELFPEK AqueK164_Aque -----------------------------------------------------------A AqueK165_Aque APAPTVTSAAKEGTF------------------------------------PPPEL---- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque -----------------------------------------------------------G AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe -----------------------------------------------------------G IRE-1_Smoe -----------------------------------------------------------G IRE2_Hsap FVQYFTNRFPRL------------------------------------------------ IRE1_Hsap FVCYFTSRFPHL------------------------------------------------ ire-1_Dmel FTNYWVDRFPQL------------------------------------------------ IRE2_Mmus FIQYFTQRFPRL------------------------------------------------ IRE1_Mmus FVRYFTSRFPHL------------------------------------------------ ire-1_Cele FLHYFTSRFPRL------------------------------------------------ IRE1_Scer FYDYFTKRFPNL------------------------------------------------ IreA_Ddis FFNYFDLKFPQL------------------------------------------------ IrlA_Ddis VLLYFECKFPDL------------------------------------------------ IrlB_Ddis ILLYFESKIPNL------------------------------------------------ IrlB_C2d_Ddis ILLYFESKIPNL------------------------------------------------ IrlC_Ddis VLSYFEEKVPDL------------------------------------------------ IrlD_Ddis VLSYFEEKVPDL------------------------------------------------ IrlE_Ddis ALNYFESKFPTL------------------------------------------------ IrlF_Ddis ILFYFESKIPNL------------------------------------------------ IrlF_C2d_Ddis ILFYFESKIPNL------------------------------------------------ 10577_Tthe MFNYFDERFPKL------------------------------------------------ IRE1_Spur FVKYFTSRFPLL------------------------------------------------ CC1G_06931_Ccin FLAYFTKRFPRL------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ----VTHRFPML------------------------------------------------ AqueK161_Aque --------------------IKTGDELVAVNDLIIHNVTKDYATEALTNVQGAFKLHPIT AqueK162_Aque -MLNMSHSLPRY------------------------------------------------ AqueK163_Aque GEKEYETKLPEE------------------------------------------------ AqueK164_Aque VV---------------------------------------------------------- AqueK165_Aque --------FPELNKKEYETKL--------------------------------------- AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque -VENI------------------------------------------------------- AqueK168_Aque YVTYFTSRFPAL------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe YESYFRTRFPKL------------------------------------------------ IRE-1_Smoe YESYFRTRFPKL------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ITPTFYCNSNTPPMLPYIKEHSEHTLSASDLFRSISARLKDSISSDLFVVSTSLYAKNLI AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque TTQLHQEITGAHGSNSNKAAKLVNVLQDSLDAHTNPEAYLQEVCSALKDVGQKQIVDVVH AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ELEPTLSISMVQLNDSSSASIPTDEHKETPVTDRVQRIKKWLEEGEVELEITRINMHGAP AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque GATATATDKIDIEKLENWLKEGKVVLEITRINMHGLPGAGKTCSQRLLLNEPPPAPPGKL AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque TDSTPIACRAVQATRISIDDKSKKWKRVEIEDLLKEL----------------------- AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ----------------------------HVKGIIKDFRVLLMTATEVELRGVLGYLKPLD AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque -------------------------SEGHVKDIVKDSRILLMTATDVELRGVLGYLKPLD AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque -------------------------------------EYLLMTATATELRGVLGHLKPLD AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------LLHT--HRA--- IRE1_Hsap ------------------------------------------------LAHT--YRA--- ire-1_Dmel ------------------------------------------------ISHA--YHA--- IRE2_Mmus ------------------------------------------------LLHT--HRA--- IRE1_Mmus ------------------------------------------------LSHT--YQA--- ire-1_Cele ------------------------------------------------LLHV--YKA--- IRE1_Scer ------------------------------------------------LIGV--YMI--- IreA_Ddis ------------------------------------------------FIVT--YLF--- IrlA_Ddis ------------------------------------------------LFHL--YQQ--- IrlB_Ddis ------------------------------------------------IHHI--YQQ--- IrlB_C2d_Ddis ------------------------------------------------IHHI--YQQ--- IrlC_Ddis ------------------------------------------------LLFL--YQK--- IrlD_Ddis ------------------------------------------------LLFL--YQK--- IrlE_Ddis ------------------------------------------------IIFL--FNH--- IrlF_Ddis ------------------------------------------------IYHL--YKQ--- IrlF_C2d_Ddis ------------------------------------------------IYHL--YKQ--- 10577_Tthe ------------------------------------------------FIFM--YYF--- IRE1_Spur ------------------------------------------------LLHV--YDA--- CC1G_06931_Ccin ------------------------------------------------FLHV--HRV--- AqueK159_Aque -------------------------------------------------------NP-FP AqueK160_Aque ------------------------------------------------LMHV--YNQ--- AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------IVDH--Y----- AqueK163_Aque GRDRIIKSFINGTWIYVGMYGGYSVVVGKSADSKGQQGGLDALIITNKIMEI--YKPRYI AqueK164_Aque ------------------------------------------------TVGI--FRG--- AqueK165_Aque GWDKVIKSFINNTRIYVGKCGGYSVVVGRSANAKSQQGGLDALTITNKIMEI--FKPKYI AqueK166_Aque ------------------------------------ETVMPSLTSQDTVATQ--ETVAIQ AqueK167_Aque GQNKIIRAFVNATWIYIGKFGQQVVVVGKSAHGEAEQGGLDAATVTKKIMAINQFKPKYI AqueK168_Aque ------------------------------------------------LVHT--YKQ--- AqueK169_Aque ------------------------------------------------LQHI--YTK--- IRE-2_Smoe ------------------------------------------------LMEV--YKV--- IRE-1_Smoe ------------------------------------------------LMEV--YKV--- IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque LK---------------------------------------------------------- AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ---------------------------------------------------------ASH AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque IAVGICFGMDHSKVNLGDVIVSNFIVDFFNFRKQPESITARKPQPPAGRTLLRFFGETSK AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque IAVGICFGMDRSKVNLGDVIVSDFIVDLSDFRKEPGSIIPRKPQPPAGTTLSSFFSDISG AqueK166_Aque ET---------------------------------------------------------- AqueK167_Aque ISIGICFGMDRSTVQLGDVIVSNMIVDLSTMDKEKDKVKARKSQPPAGPSLLSLFGDSSG AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap --------------------------------------------------------M-R- IRE1_Hsap --------------------------------------------------------M-E- ire-1_Dmel --------------------------------------------------------F-S- IRE2_Mmus --------------------------------------------------------M-R- IRE1_Mmus --------------------------------------------------------M-E- ire-1_Cele --------------------------------------------------------T-E- IRE1_Scer --------------------------------------------------------V-K- IreA_Ddis --------------------------------------------------------I-L- IrlA_Ddis --------------------------------------------------------F-K- IrlB_Ddis --------------------------------------------------------L-K- IrlB_C2d_Ddis --------------------------------------------------------L-K- IrlC_Ddis --------------------------------------------------------F-K- IrlD_Ddis --------------------------------------------------------F-K- IrlE_Ddis --------------------------------------------------------F-S- IrlF_Ddis --------------------------------------------------------L-K- IrlF_C2d_Ddis --------------------------------------------------------L-K- 10577_Tthe --------------------------------------------------------S-Q- IRE1_Spur --------------------------------------------------------L-S- CC1G_06931_Ccin --------------------------------------------------------I-S- AqueK159_Aque -------------------------------------------------------VLTT- AqueK160_Aque --------------------------------------------------------M-K- AqueK161_Aque LKKKMKDEEISTEVDKAPKPKIDAPNHSDELPLAKPIKNEPAPTKNEP-VLTKNEPVSTE AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque FEIKHSDQENPKEVE-----------------------VHCGPIASTP-ALVDDPKFKEE AqueK164_Aque --------------------------------------------------------L-R- AqueK165_Aque FKMKHSQEENAKEVN-----------------------VHCGPIASSP-VLVDDEEFKEQ AqueK166_Aque -------------------------------------------------------EGQA- AqueK167_Aque FQKKHSAEENAKEVK-----------------------VHCGPVVSSFVSLVDDAEFKEQ AqueK168_Aque --------------------------------------------------------M-E- AqueK169_Aque --------------------------------------------------------I--- IRE-2_Smoe --------------------------------------------------------I-A- IRE-1_Smoe --------------------------------------------------------I-A- IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque NQLVSTKNEPAPAENELAPAENEEAKNKPTENAESTKINQDTDMKVIKEIADSLADRKFQ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque LREVRPDTLGGEMEGAAILHA-------------ASEAHHKVEAIVIKAVGDL-ADGKKD AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque LSKARPDALAGEMEGAAIFSA-------------ARHAHHKPEVIVIKAVGDW-GDGKKD AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque LKEVRPDALGGEMEGAGIFLA-------------ARDAHHKVEAIVIKAVADW-GDGRKD AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap -----------S------------------------------------------------ IRE1_Hsap -----------L------------------------------------------------ ire-1_Dmel -----------I------------------------------------------------ IRE2_Mmus -----------T------------------------------------------------ IRE1_Mmus -----------L------------------------------------------------ ire-1_Cele -----------Y------------------------------------------------ IRE1_Scer -----------E------------------------------------------------ IreA_Ddis -----------K------------------------------------------------ IrlA_Ddis -----------K------------------------------------------------ IrlB_Ddis -----------Q------------------------------------------------ IrlB_C2d_Ddis -----------Q------------------------------------------------ IrlC_Ddis -----------K------------------------------------------------ IrlD_Ddis -----------K------------------------------------------------ IrlE_Ddis -----------A------------------------------------------------ IrlF_Ddis -----------E------------------------------------------------ IrlF_C2d_Ddis -----------E------------------------------------------------ 10577_Tthe -----------T------------------------------------------------ IRE1_Spur -----------L------------------------------------------------ CC1G_06931_Ccin -----------D------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque -----------Y------------------------------------------------ AqueK161_Aque ELSTNWVYFIDSGGQPAYRELLPLFTRAAALNIITIDLTKGLDKNCEFQYRISQNMFPIK AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque E-CRGWKDFASH------------------------------------------------ AqueK164_Aque -----------V------------------------------------------------ AqueK165_Aque E-CRGWKDFASH------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque E-CRGWKDFASY------------------------------------------------ AqueK168_Aque -----------M------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe -----------D------------------------------------------------ IRE-1_Smoe -----------D------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel ------------------------------------------------------------ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele ------------------------------------------------------------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ------------------------------------------------------------ CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque TKLEYSNLDIIQSTISSEAMLNPIEIPYVSESDMPKHPHYLILGTRIDELKEKGMLNRLD AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap -------------------------------------------CASES---LFLPYYPPD IRE1_Hsap -------------------------------------------CSHER---LFQPYYFHE ire-1_Dmel -------------------------------------------CSNEP---IFKPYYSAG IRE2_Mmus -------------------------------------------CASES---LFLPYYPPA IRE1_Mmus -------------------------------------------CRHER---LFQTYYWHE ire-1_Cele -------------------------------------------CSGEA---VFKRYYSDD IRE1_Scer -----------------------------------------NL-SDDQ---ILREF---- IreA_Ddis -----------------------------------------NL-KNDQ---YF------- IrlA_Ddis -----------------------------------------SD-FNSK---DY------- IrlB_Ddis -----------------------------------------YS-LTID---YL------- IrlB_C2d_Ddis -----------------------------------------YS-LTID---YL------- IrlC_Ddis -----------------------------------------HL-DSKS---LI--YF--- IrlD_Ddis -----------------------------------------HL-DSKS---LI--YF--- IrlE_Ddis -----------------------------------------IP-EIKN---SI--HFSN- IrlF_Ddis -----------------------------------------YS-SSFD---YL------- IrlF_C2d_Ddis -----------------------------------------YS-SSFD---YL------- 10577_Tthe ------------------------------------------Q--NWE---LL------- IRE1_Spur -------------------------------------------CREEP---VLSKYYYNS CC1G_06931_Ccin -----------------------------------------TVLRTES---MFRSYFE-- AqueK159_Aque -----------------------------------------------Y-----DRTN--- AqueK160_Aque -------------------------------------------YDK-T-----LSYY--- AqueK161_Aque NMNKKLINEGFNNLIKRNEGKGSVIFPVNTLLLAGSKEREKASEEL-C-----TEVS--- AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque -------------------------------------------AAATFVHD--KLNI--- AqueK164_Aque -----------------------------------------A--VKSL---HT------- AqueK165_Aque -------------------------------------------AAATYIHH--HLND--- AqueK166_Aque -----------------------------------------------T-----SVPS--- AqueK167_Aque -------------------------------------------AAATYVHHQMNKVV--- AqueK168_Aque -------------------------------------------CCNEP---VFKAYYS-- AqueK169_Aque -----------------------------------------------Y-----VEYV--- IRE-2_Smoe -----------------------------------------HC-WEEE---MFQPYFSE- IRE-1_Smoe -----------------------------------------HC-WEEE---MFQPYFSE- IRE2_Hsap SEARR------------------------------------------------------- IRE1_Hsap PPEPQ------------------------------------------------------- ire-1_Dmel YLFTRPWYFDADDALFPMLMLDPKPLPKIGS------PKKTPSPASSQAQQLKQRKGLYN IRE2_Mmus LEAR-------------------------------------------------------- IRE1_Mmus PTEPQ------------------------------------------------------- ire-1_Cele VRARM---------Y-PIVEEEERVRKKIKEEMANEVWARAPKPVE-------Q------ IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur SKKDV---------H----------------------------TAA-------------- CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap ------------------------------------------------------------ IRE1_Hsap ------------------------------------------------------------ ire-1_Dmel FRKPNDELPIPGVGLQRNLELDGQSLEPDGKRDVFANFKFRRYSKPGNNRNYNGGHKEAQ IRE2_Mmus ------------------------------------------------------------ IRE1_Mmus ------------------------------------------------------------ ire-1_Cele --------------------------------------------------------RTPL IRE1_Scer ------------------------------------------------------------ IreA_Ddis ------------------------------------------------------------ IrlA_Ddis ------------------------------------------------------------ IrlB_Ddis ------------------------------------------------------------ IrlB_C2d_Ddis ------------------------------------------------------------ IrlC_Ddis ------------------------------------------------------------ IrlD_Ddis ------------------------------------------------------------ IrlE_Ddis ------------------------------------------------------------ IrlF_Ddis ------------------------------------------------------------ IrlF_C2d_Ddis ------------------------------------------------------------ 10577_Tthe ------------------------------------------------------------ IRE1_Spur ----------------------------------------------------------AM CC1G_06931_Ccin ------------------------------------------------------------ AqueK159_Aque ------------------------------------------------------------ AqueK160_Aque ------------------------------------------------------------ AqueK161_Aque ------------------------------------------------------------ AqueK162_Aque ------------------------------------------------------------ AqueK163_Aque ------------------------------------------------------------ AqueK164_Aque ------------------------------------------------------------ AqueK165_Aque ------------------------------------------------------------ AqueK166_Aque ------------------------------------------------------------ AqueK167_Aque ------------------------------------------------------------ AqueK168_Aque ------------------------------------------------------------ AqueK169_Aque ------------------------------------------------------------ IRE-2_Smoe ------------------------------------------------------------ IRE-1_Smoe ------------------------------------------------------------ IRE2_Hsap --------PCPGATGR IRE1_Hsap --------PPVTPDAL ire-1_Dmel DKEKYVIWTLPPSTQD IRE2_Mmus ---------RPDATKS IRE1_Mmus --------PPVIPYAL ire-1_Cele KLDKRNIKKKSNPNTD IRE1_Scer -------------LYS IreA_Ddis -----------VQYYY IrlA_Ddis -----------LNIKF IrlB_Ddis -----------FNFYN IrlB_C2d_Ddis -----------FNFYN IrlC_Ddis ----------NDLIIK IrlD_Ddis ----------NDLIIK IrlE_Ddis ----------DTCSIF IrlF_Ddis -----------IKFYN IrlF_C2d_Ddis -----------IKFYN 10577_Tthe -----------SFKNN IRE1_Spur SEGDH-IQGVHSNPSQ CC1G_06931_Ccin ------------LPDS AqueK159_Aque ---------QGDCSSN AqueK160_Aque ---------YQISLII AqueK161_Aque ---------NCRVAIK AqueK162_Aque ---------IRLLANL AqueK163_Aque ---------AAPDALK AqueK164_Aque -----------IIISD AqueK165_Aque ---------AAPDALK AqueK166_Aque ---------QSTLTCK AqueK167_Aque ---------HAGARRP AqueK168_Aque -------------RSS AqueK169_Aque ---------IKNPHYK IRE-2_Smoe -----------ADDYL IRE-1_Smoe -----------ADDYL