Gene BARK1 (Mouse)

BARK1

Species: Mouse
Alias: Adrbk1, BARK1
External Links: MGI , Entrez Gene , Refseq cDNA , Refseq Protein , Phosphosite , iHOP
Annotation:

Classification

Group: AGC
Family: GRK
Subfamily: BARK

Sequence

Name Sequence Type Origin Length Description Download
BARK1.AA Protein Sugen 689 None Fasta, JSON
BARK1.NA RNA Sugen 2361 None Fasta, JSON
BARK1.kin_dom Protein Kinase Domain Sugen global kinase HMM prediction 263 Kinase domain by global Sugen HMM Fasta, JSON

Protein domain

Protein domains of BARK1.AA. The domains are annotated by HMM profiles from Pfam and SMART, as well as in-house data which includes HMMs of each individual kinase group, family and subfamily. Here is domain list in details, including sequence identity, significance and alignment. In particular, you can find can find the best hit kinase group/family/subfamily, which is helpful to understand the relationship between kinases.Kinase domain and best hit kinase group/family/subfamily are highlighted in red and blue. Visualized by pviz.

Usage: Zoom in selected region by dragging mouse and zoom out by double-clicking.

Show/hide domain list
Domain Protein name Domain Name Range Identity (%) Significance Score Profile Source Profile Range (length) Alignment
RGS BARK1.AA RGS 54-175 25 1.4e-32 115.47 SMART 1-121 (121) Show / Hide 
Range on Protein: 54-175
Range on HMM: 1-121/121
Sequence Identity: 25% (32 aa)

TFEKIF-SQKLGYLLFRDFCLNHLEEAKPLVEFYEEIKKYEKLETEEERVVRSREIFDSYIMKELLA-CSHPFSKNATEHVQGHLVKKQVPPDLFQPYIE
.|.... ... |...||.|. .  .|.|...||........|.|. |||.   |||.| ||... .  ... ...   |..| .| .  ..||.|... .
SFHHLCDNDPIGRRYFREFLKQEFCEYKENLEFWLACEEFKKMED-EERRQKAREIYDKYISPNAPCEHCVNIDHHTREQCQQCLCN--PHPDCFDEAQR

EICQNLRGDVFQKFIESDKFTRFC
...| ...| ...|..||...|..
HVYQLMEKDSYPRFLRSDYYKRYL
RGS BARK1.AA RGS 54-174 25 1.1e-29 99.93 Pfam 1-124 (124) Show / Hide 
Range on Protein: 54-174
Range on HMM: 1-124/124
Sequence Identity: 25% (32 aa)

TFEKIFSQKL-GYLLFRDFCLNHLEEAKPLVEFYEEIKKYEKL--ETEEERVVR-SREIFDSYIMKELLACSHPFSKNATEHVQGHLVKKQVPPDLFQPY
.|..... .  | .|||.|. .   |  ...||........|   ...|..  .  |||.. ||. .     |  .... ||.| .| ..|. ||.|...
SFHHLCNDQPIGRRLFRQFLESEFCEY-ENIEFWLACEDFKKMFSDSDEKMHQKDAREIYNKYIAPDSPCECHNIDHDTREHCQDNLEQAQPTPDCFDEA

IEEICQNLRGDVFQKFIESDKFTRF
...|.. ...| ...|..||.. ||
QRHIYTLMEKDSYPRFLKSDYYQRF
Kinase BARK1.AA BARK 191-453 93 1e-228 766.12 In-house 1-264 (264) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-264/264
Sequence Identity: 93% (247 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTG-DCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVF
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||.|||||||||.|||||||||||||||||||||
FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGQDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVF

SEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKRPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHS
||..||||||||||||||||||||||||||||||||||.|||||||||||||||||.|||||||||||||||||||.|||||||||||||||.|||||||
SEQEMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDENGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLYKLLRGHS

PFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFF
||||||||||||||||||||.||||||||.|.|.||||||||||..|||| |||||||||.|||
PFRQHKTKDKHEIDRMTLTMNVELPDSFSKEMRDLLEGLLQRDVSKRLGCMGRGAQEVKEHPFF
Kinase BARK1.AA GRK 191-453 60 3.5e-216 688.39 In-house 1-272 (272) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-272/272
Sequence Identity: 60% (164 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGV--
|...|..|.||||||..|....||||||.|||||||||...|||.||||...|..|...||||||||.||..|.|.| .....|||||||||....|.  
FRQYRVLGKGGFGEVCACQVRATGKMYACKCLDKKRIKKRKGETMALNEKQILEKVHSRDCPFIVCMAYAYETKDALCLVMTIMNGGDLHYHIYNMGNNE

--FSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKR--PHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKL
  |.|..||||||||..||||||.|..|||||||.|||||.|||||||||||||.....   .|..|||||||||||||...|||.|.||.|||||....
PGFDEERMRFYAAEICCGLEHMHRRRIVYRDLKPENILLDDHGHVRISDLGLACEIPEGQPMIHGRVGTHGYMAPEVLQNERAYDFSPDWWSLGCMIYEM

LRGHSPFRQHKTKDKH-EIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGR--GAQEVKESPFF
..|||||||||.|.|. |.||......|..||.||.|.....|.|||.|...|||| |.  ||||||| |||
IEGHSPFRQHKEKVKREEVDRRVKEDPVKYPDKFSEEAKDICEMLLQKDPEQRLGCRGGTCGAQEVKEHPFF
Kinase BARK1.AA AGC 191-453 28 1e-151 395.46 In-house 1-395 (395) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-395/395
Sequence Identity: 28% (114 aa)

FSVHRIIGRGGFGEVYGCRKA------DTGKMYAMKCLDKKR-IKMKQ--GETLALNE------------RIMLSLVSTGD-------CPFIVC-MSYAF
| ....||.|.||.|| .||       ||||.||||.|.||. |..|.   .  ...|            | .|....          .||||  . |.|
FEFLKVIGKGSFGKVYLVRKKDGCHKKDTGKYYAMKVLKKKHKIVIKKKNMVEHTMTEHWKQDQGYVWEERNILEKCNHTHNHPIKSHHPFIVKILHYSF

HTPDK--LSFILDLMN---------GG-DLHYHLS---QHGVFSEADMRFYAAE-------IILGLEHMHN-RFVVYRD-LKPANILLDEHGHVRISDLG
...|.  | |....|.         || .|..||    . |.|.|   ||||||       |.|.||..|. . ...|| ||| ||||||.||....| |
QDKDHTYLYFVMEYMPRYVNDLYCGGGCELFLHLQGRRKEGRFDEERARFYAAEMQKICGCIVLALEYLHSHMGIIHRDYLKPENILLDEDGHIKLTDFG

LACDFSK----------------------------------------------------KRPHASVG--THGYMAPEVLQKGVAYDSSADWFS--LGCML
|.. . .                                                    .| .  .|  |. |||||.|     |. ..|| |  |||..
LCKEGMNDGEKGQQSQSNMKFHWHNKKYQKSNMDTNKCNPNNWQRNNMSQEDDTTRKKRDRTWTFCGPCTPEYMAPEIL-----YGKACDWWSFGLGCLM

FKLLRGHSPFRQH-------------KTKDKHEIDRMTLTMAVELP----------DSFSPELRSLLEGLLQRDVNRRLGCLGR-----GAQEVKESPFF
. .| |..||. .             .. .| ...|....  |..|          ..||||...|...||..| ..||||.|.     ||.|.|. |.|
YEMLTGYPPFYGDNPQENRHPFRNPMEMFQKIMNGRYFINWKVKFPFPEDPQFPEDRWFSPEAKDLIKKLLCKDPEKRLGCGGNDFGDKGAEEIKNHPWF
Kinase BARK1.AA GRK 191-453 46 1e-103 350.9 In-house 1-265 (265) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-265/265
Sequence Identity: 46% (125 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHG---
|   |. | ||||||  |    |||||| | |.||||| ..||..||||   |  |.    .|.| . ||. | | |  .. .|||||| .|.   |   
FRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEQMALNEKQILEKVH---SRFVVSLAYAYETKDALCLVMTIMNGGDLKFHIYNMGENP

VFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKRP-HASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLR
 |.|.  .||||||..|||| | . .||||.|| |||||.|||.||||||||       .    ||| |||||||..  . |. | ||..||| ..    
GFDEERAIFYAAEICCGLEHLHRERIVYRDMKPENILLDDHGHIRISDLGLAVEIPEGQMIRGRVGTVGYMAPEVVKNER-YTFSPDWWGLGCLIYEMIE

GHSPFRQHKTK-DKHEIDRMTLTMAVELP-DSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFF
|.||||| | |  . |.|| ..   .. . . || | ... . ||. |   |||| . |..||.. |||
GRSPFRQRKEKVKREEVDRRVKEDPEKYSHEKFSEEAKDICRMLLHKDPEQRLGCREEGCDEVRQHPFF
Kinase BARK1.AA Pkinase 191-453 28 3.2e-97 334.09 Pfam 1-293 (293) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-293/293
Sequence Identity: 28% (86 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLAL--NERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGG----DLHYHLS
. ..|.||.|.||.|| |.. .|||..|.|...|...|... .|...    .||..| .   .|.|| . ..|...|.. .......||    ||. ..|
YHIGRKIGSGSFGCVYKCHHKGTGKIVAVKIIKKRHEKSWKDQTHRRLSEIQIMKRL-N---HPNIVRLYDWFEDKDHIYMVMEYCEGGLYVMDLFDYIS

--QHGVF--SEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHV----RISDLGLAC---DFSKKRPHASVGTHGYMAPEVLQKGVAYDSSAD
  .||.|  || . .||. .|. |||..|.. ...|||||.|||.|..||.    .|.| |||.     ..  ..  .|| .||||||...|  |....|
TWRHGCFYFSEWECKFYMYQILRGLEYCHSMGIIHRDLKPENILIDNNGHIDACVKICDFGLAKQFDY-NNS-MTTFCGTPWYMAPEVIRGGQYYGPKVD

WFSLGCMLFKLLRGHSPFRQH----KTKDKHEIDRMTLTMAVELPDSFS---------------------PELRSLLEGLLQRDVNRRLGCLGRGAQEVK
..|.||.|. .| |. ||..|    .  |. .. ....   .. . . .                     .|........|..| ..|..     |....
MWSCGCILYEMLCGRPPFPGHCWHDN-HDQMQMICRIMGPPLHFDWPWWCSISYFFFRHWHFHWTFHNWSEECKDFIKWCLCKDPKKRPT-----AEQIL

ESPFF
..|.|
QHPWF
Kinase BARK1.AA RSK 194-453 35 1.2e-91 310.79 In-house 1-278 (278) Show / Hide 
Range on Protein: 194-453
Range on HMM: 1-278/278
Sequence Identity: 35% (99 aa)

HRIIGRGGFGEVYGCRKA---DTGKMYAMKCLDKKRIKMKQG-ETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGV-
..  |.||.|.|. |||    |||. |||| |.|. |. .|     .. ||  |...  . .||||   |||.|..||  |.....||.| .||....  
LKVLGQGGYGKVFLCRKCTHHDTGQIYAMKVLKKAKIVQRQKDTRHTMTERNILERC--QQHPFIVTLHYAFQTEGKLYLIMEYCRGGELFTHLQKEKHF

--FSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDE---HGHVRISDLGLACDF--S-KKRPHASVGTHGYMAPEVLQ---KGVAYDSSADWFSL
  ||| . .||.|||.| .||.| . ...||||| |||.|.   .||....| |... |  . ... .  ..|. |||||.|    .....| ..||.||
GCFSEDEACFYMAEIVLAVEHLHQQGIIHRDLKPENILYDDESNEGHIKLTDFGFCKQFRCDHNQWTMTFCYTIEYMAPEMLKRANQQGGHDKACDWWSL

GCMLFKLLRGHSPFRQHKTK-DKHEIDRMTLTMA--VELP--DSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFF
|. .. .| |..||..   . ...|| .  ..    ...|  .. |.| ..|. ..|.|| ..||||  ..|.|.|..|.|
GVLMYEMLTGCPPFQG-PNDNNRKEIMKRIQKGKFSLTMPNWQYLSQEAKDLIRKMLHRDPHKRLGCGPQDAEEIKQHPWF
Kinase BARK1.AA S_TKc 191-453 30 1.5e-88 301.42 SMART 1-231 (231) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-231/231
Sequence Identity: 30% (83 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNG--GDLHYHLSQHGV
. ..|.||.|.||.|| ||. .||. .|.|...            ...|. .|.     ..|.|| . ..|.. |.| .......|  |||. .. ..| 
YEILRKIGKGAFGKVYKCRHKKTGRIVAIKIIK----------EHIRREIQILKK----HHPNIVKLYDVFQD-DHLYMVMEYCDGDLGDLFDYIKKRGR

------FSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKRPHASVGTHGYMAPEVLQKGVAYDS-SADWFSLGCML
      |.| . |||...|...||..|.. ...|||||.||||||  |. |.| |||. .     .  .||  |||||||     |.  ..|| |.||.|
HGLRFPFPE-HARFYMYQICCALEYCHSHGIIHRDLKPENILLDE--HIKICDFGLARQL-----TTFCGTPWYMAPEVL----GYGKCKCDWWSCGCIL

FKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGA--QEVKESPFF
. .| |  ||.     ..  ....            |||........||.| ..|...    .  ...|  |.|
YEMLCGYPPFP----QMQMMFKKIG-----------SPEAKDFIRKCLQKDPEKRPTA-EALQDEWDIKCHPWF
Kinase BARK1.AA AktR 191-453 36 2.9e-80 272.94 In-house 1-268 (268) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-268/268
Sequence Identity: 36% (99 aa)

FSVHRIIGRGGFGEVYGCRKA-DTGKMYAMKCLDKKRIKMKQGETLALNERIMLS-LVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGV
| |....|.|.||.|| ..|  |..|.|||| ..|| |. .. .  ...||  .  ..   . |||| . |.| | .|| ||.. . ||.| .||.. . 
FHVYKCLGKGAFGKVYLVQKKRDNQKQYAMKMIKKKKIIERNQMHHTITERNIMEMCC---NHPFIVKLHYCFQTKEKLFFIMEYCPGGELFQHLKKKKK

FSEADMRFYAAEIILGLEHMHNR-FVVYRDLKPANILLDEHGHVRISDLGLAC-DFSK-KRPHAS--VGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFK
||| . |||.|.|.|.||. |..  ..|||||| ||||||.|||.|.| ||.   | . ....|   .||. |||||...| . .. |.|| ..|. .. 
FSEEQARFYFAQILLALEYLHQHMNIIYRDLKPENILLDEDGHVKITDFGLSKLQFMMHYDEKAYSFCGTPEYMAPEMITKKKGHNKSVDWWAFGIFIYE

LLRGHSPFRQHKTKDKHEIDRMTLTMA-VELPDS--FSPELRSLLE-GLLQRDVNRRLGCLGRGAQEVKESPFF
.. |. || ....... .   . ..   .. |..  ||.....|.. .||... | |||    |..|.|. |.|
MVTGCPPFYHKNQNQMFQ---KIKIKQQNKFPYPSYFSSQCKDLIQRKLLCKNPNKRLGYYCNGWEEIKSHPWF
Kinase BARK1.AA SGK 191-453 35 1.2e-79 245.56 In-house 1-283 (283) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-283/283
Sequence Identity: 35% (104 aa)

FSVHRIIGRGGFGEVYGCRKADT------------------GKMYAMKCLDKKRIK-------MKQGETLALNER-IMLSLVSTGDCPFIVCMSYAFHTP
|    .|| |.||.||  .. |.                  .|.|||| | |. |         |  . . . ||   |....    ||||   ||| |.
FDFLKVIGKGSFGKVYQVKHKDDETCQRVWLIFQSLLSQSEDKFYAMKVLQKQHIINEYRQDLKKKEQKHIMAERNVILKNMK---HPFIVNLHYAFQTK

DKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLAC-DFSKKRPHASV-GTHGYMAPEVL
.|| | .| .|||.. |||   . |.|   |||||||.. ||. |   ..|||||| |||||  ||... | ||.  .  ..    .  ||  |.|||||
WKLYFVMDYVNGGEMFYHLQREKHFPEDRARFYAAEIVCALEYLHSQDIIYRDLKPENILLDHQGHCCLTDFGLCKEGIKTNDTTFTFCGTPEYLAPEVL

QKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFF
.|   .| . ||..||| |. .. |  ||     ..| .     .     .| ..|.. ..|..||||.|.  |||   |.. |.|. |||
RK-QPHDRTVDWWCLGCVLYEMMYGLPPFYS---RNKQQMYDNIINQPLQIPPQMSNDAWDLIQGLLQKDPKKRLGTK-RDFMEIKNHPFF
Kinase BARK1.AA RSK 191-453 36 9.5e-72 244.67 In-house 1-271 (271) Show / Hide 
Range on Protein: 191-453
Range on HMM: 1-271/271
Sequence Identity: 36% (104 aa)

FSVHRIIGRGGFGEVYGCRKA---DTGKMYAMKCLDKKRIKMK-QGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQH
. .|. ||.| |..|| |.|    ||.. |||| ..|. .. | .    . .|||.|. .   . ||||   |.| .  |.  |.|.| ||||  .|.  
YELHECIGQGSFSKVYVCKKCQHKDTNQHYAMKIIKKATRDVKDR--VCWEEERIILRYG---HHPFIVKLHYVFQDEGKVYMIMDFMRGGDLFDRLLKQ

GVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEH----GHVRISDLGLACDFSKKRPHASVG-------THGYMAPEVLQKGVAYDSSADWF
..||| . .||.||| | .|. |   .|.|||||.|||.|..    ||..| |.|.|     |   .  |       |  ||||||| .   .| ..||.
KMFSEREVKFYMAEIALAMEYLHSQGIVHRDLKPENILYDDESGNPGHIKICDFGFA-----KQLRDENGMLMTFCYTANYMAPEVLKR-QGHDQACDWW

SLGCMLFKLLRGHSPFRQHKTKDKHE--IDRMTLTM---AVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFF
|.|  ..  | |. || |.|..|  .  | |  | |       .|. |.|  .|| ..| || ..|||.   |....|. |.|
SFGVLMYEMLTGCTPF-QNKPRDTPMNIIQRIKLGMFSMTHGNWDFISDEAKDLLRMMLHRDPHNRLGAGPNGVEQIKQHPWF
Kinase BARK1.AA MSK 191-448 32 3.4e-69 233.07 In-house 1-277 (277) Show / Hide 
Range on Protein: 191-448
Range on HMM: 1-277/277
Sequence Identity: 32% (92 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLAL--NERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGV
 .|   .| | |. .  |.. |||| |||| | | ..  |.  | .    ||  | ..    .|.||   |||... ..  .... ||| |  |... . 
LKVLGTGGYGKFFICRKCVHHDTGKLYAMKILSKATVVQKR-KTAEHTQTERQCLEMCQQ--HPNIVNLHYAFQDQCHTYLVMEYCNGGELFTHIRKREH

FSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEH---GHVRISDLGLACDFSKK--RPHAS-VGTHGYMAPEVLQK---GVA-YDSSADWFSLG
|.|.  |.. .|... ..||| . ...||||| |||.|.    |||...| |.|. | .    .  |  .|  |||||.|..   .   ||.. ||.|||
FDESECRMIMRELVMAVDHMHQKGIIHRDLKPENILFDDEEDNGHVKLIDFGFARLFPQDEEWRMKSPCFTLHYMAPEMLRRGYAQDGGYDEACDWWSLG

CMLFKLLRGHSPFR------QHKTKDKHEIDRMTLTMAVELP----DSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVK
  |. .| |..||.      ||. .  ||| .  ..  . .|     ..||. . |...||..| ..|||. ...|.|.|
VILYTMLTGQVPFQQNEDDRQHSCNSHHEIMKRICKGEFSFPGMAWQNMSPDAKDLIQWLLTKDPKKRLGMNDLRAHEWK
Kinase BARK1.AA Akt 191-339 45 4.5e-61 193.94 In-house 1-148 (264) Show / Hide 
Range on Protein: 191-339
Range on HMM: 1-148/264
Sequence Identity: 45% (69 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMY-AMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVF
| . ...|.| || |. ..| ||.|.| ||| | |..| .|     .. || .|  .    .|||| . |.| |.||| |..| .|||.| |||.. ..|
FDFLKVLGKGSFGKVFLVKKKDTQKWYYAMKVLKKDYIIQKNQVEHTKTERNILQHCN---HPFIVNLHYSFQTQDKLYFVMDYCNGGELFYHLQRKRRF

SEADMRFYAAEIILGLEHMHNRFVVYRD-LKPANILLDEHGHVRISDLGLA
||   |||.|||||..|| |.. ..||| ||| |||.|..||... | ||.
SEDRARFYCAEIILAIEHLHSKNIIYRDYLKPENILMDKDGHICLTDFGLC
Kinase BARK1.AA Akt 356-453 34 2e-21 65.2 In-house 168-264 (264) Show / Hide 
Range on Protein: 356-453
Range on HMM: 168-264/264
Sequence Identity: 34% (35 aa)

YMAPEVLQKGVAYDSSADWFS-LGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELP--DSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPF
|.|||.|.. . .. ..|| . ||| .. .. |  ||  .. .|.|.     ..  |. |  | .||| ..|..|||..| ..|||    .|||.|. |.
YLAPEILNG-NGHTKAVDWWGSLGCVMYEMMCGRPPF--YN-QDHHQMFKKIKNQPVKFPDKDWISPECKDLISGLLNKDPKKRLGGGPNDAQEIKRHPW

F
|
F
Kinase BARK1.AA TyrKc 191-440 20 1.1e-11 -33.92 SMART 1-280 (280) Show / Hide 
Range on Protein: 191-440
Range on HMM: 1-280/280
Sequence Identity: 20% (61 aa)

FSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNE-RIMLSLVSTG-DCPFIVCMSYAFHTP-------DKLSFILDLMNGGDLHY
 .  ...|.| ||||| ..      . | |.| .        .   ..| ||| .|   | . | ||..  .  ..       . |  .. .| .|||. 
ITLGKKLGEGAFGEVYKGTWK---IQVAVKMLKEDA------REDFMREARIMCKL---GGHHPNIVRLYGVCTQQGRNFMYIEPLMIVMEYMPHGDLKD

HLSQHGV-FSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKK------RPHASV----GTHG-----YMAPEVLQKG
.|  |.  .. .|.  ..  |  | |. | . .. |||   |.|..| ..|.||| ||. |   .          ..    |...      ||||....|
YLRKHRPKLCMKDLLSFCWQIACGMEYLHSKNCIHRDLAARNCLVGENHTVKISDFGLSRDIYDDDQQGESKDYYRKKEHKGGKTMLPIRWMAPECINYG

VAYDSSADWFSLGCMLFKLL-RGHSPFRQ---HKTKDKHEIDRMTLTMAVELPDS--------FSPELRSLLEGLLQRDVNRR------LGCL
. . .  | .| | ... .. .| .|      .. .... | .    .  . |..         ..|. .. .   ..| . |        |.
K-FTTKSDVWSFGVCMWEIFTYGQQPYPGMIQQMMSNQEVIEYVKQGYRMPQPPNDYPMSFVCCPDEMYQIMKQCWHEDPEDRPTFSEIHECF
Kinase BARK1.AA S_TK_X 454-533 23 3e-11 44.63 SMART 1-79 (79) Show / Hide 
Range on Protein: 454-533
Range on HMM: 1-79/79
Sequence Identity: 23% (21 aa)

RSLDWQMVFL--QKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISER-WQQEVAET-----VFDTINAET
|..||. ..   ....|| .|.  ||.| ...|.. ||.| |..          . ... ..|.. ||.| .||      |   ..  
RGIDWDKLYNQQKEIEPPFKPKIKEVYA-SPTDTSNFDPEFTEETPM-------LTPDDPPLSNSMWQNE-METEIPPDPFRGFTYVF
Kinase BARK1.AA Pkinase_Tyr 273-339 30 0.000846 13.49 Pfam 88-158 (270) Show / Hide 
Range on Protein: 273-339
Range on HMM: 88-158/270
Sequence Identity: 30% (22 aa)

LMNGGDLHYHLSQH----GVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLA
.| .|||. .| .|    . ..  |.  ....|  |.|. ...  . |||   |.|..| ..|.|.| |||
YMPHGDLLDYLRKHREDKQPLTMKDLLQMCWQIAKGMEYLESKNCIHRDLAARNCLVTENNVVKICDFGLA
Kinase BARK1.AA Pkinase_Tyr 191-206 43 0.899233 3.24 Pfam 1-16 (270) Show / Hide 
Range on Protein: 191-206
Range on HMM: 1-16/270
Sequence Identity: 43% (7 aa)

FSVHRIIGRGGFGEVY
 .  . .|.| |||||
ITFGKKLGEGAFGEVY
PH BARK1.AA PH 559-652 22 4.4e-17 64.26 Pfam 1-92 (92) Show / Hide 
Range on Protein: 559-652
Range on HMM: 1-92/92
Sequence Identity: 22% (21 aa)

CIVHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEG-EAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYR
.| .|...|.|  .. .|..|.| ||.|.| .  .. . |.  . ....| .. .  |.. |. ... .......|..|. |. .| . ...| |
VIKEGWLHKKG--KRKSWKKRWFVLFNNCLYYYKDDKYRPKGSIPLSDCQVTKVPDNKRPHCFQIRT-DRRTYYFQAESEEEMNDWMQAIQSACR
PH BARK1.AA PH 559-654 19 8.2e-15 56.46 SMART 1-82 (82) Show / Hide 
Range on Protein: 559-654
Range on HMM: 1-82/82
Sequence Identity: 19% (19 aa)

CIVHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYREA
 | .|...|..   . .|..|.|.||...| .  .  .|.  . .... .|.|       |. ..  ..  ...||.|. |. .| . ...| ...
VIKEGWLYKKS---K-RWKKRWFVLFNDCLYYYKDK-KPKGSIPLSDC-TVRE-P-----CFEIHH-DR-TYYFQCESEEEMQEWMQAIQKAIHNN