Gene MAST4 (Mouse)

MAST4

Species: Mouse
Alias: MAST4, 4930420O11Rik
External Links: MGI , Entrez Gene , Refseq cDNA , Refseq Protein , Phosphosite , iHOP
Annotation:

Classification

Group: AGC
Family: MAST
Subfamily: MAST

Sequence

Name Sequence Type Origin Length Description Download
MAST4.kin_dom Protein Kinase Domain Sugen global kinase HMM prediction 238 Kinase domain by global Sugen HMM Fasta, JSON
MAST4.NA RNA Sugen 8163 None Fasta, JSON
MAST4.AA Protein Sugen 2630 None Fasta, JSON

Protein domain

Protein domains of MAST4.AA. The domains are annotated by HMM profiles from Pfam and SMART, as well as in-house data which includes HMMs of each individual kinase group, family and subfamily. Here is domain list in details, including sequence identity, significance and alignment. In particular, you can find can find the best hit kinase group/family/subfamily, which is helpful to understand the relationship between kinases.Kinase domain and best hit kinase group/family/subfamily are highlighted in red and blue. Visualized by pviz.

Usage: Zoom in selected region by dragging mouse and zoom out by double-clicking.

Show/hide domain list
Domain Protein name Domain Name Range Identity (%) Significance Score Profile Source Profile Range (length) Alignment
DUF1908 MAST4.AA DUF1908 268-347 66 1.3e-45 162.69 Pfam 1-93 (369) Show / Hide 
Range on Protein: 268-347
Range on HMM: 1-93/369
Sequence Identity: 66% (62 aa)

PQDSPRNFSPSASAHFSF--AR-------RTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKL----HQLPYQPTPDELHFLSKHFCTTES
| ||||||| |....| |  .|       | ||||||.|||||||||||.|||.|||.|||||.|    ||||.||| |.||||||||| |||
PIDSPRNFSTSSMMNFPFVPIRRITNCRDRADGRRWSVASLPSSGYGTNPPSSNVSSQCSSQERLNQLPHQLPFQPTQDDLHFLSKHFCCTES
DUF1908 MAST4.AA DUF1908 354-542 68 3.9e-131 446.75 Pfam 178-369 (369) Show / Hide 
Range on Protein: 354-542
Range on HMM: 178-369/369
Sequence Identity: 68% (131 aa)

CRNTPMRPRSRSL-SPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYS--PDHVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFF
||  ..||||||| ||.||.   | ||.||||||||||||||.||||||...|. ..  |.  |.|||.|. | |||..|.|||||.|||..||||||| 
CRSPHHRPRSRSLSSPSRSCITFDNEIVMMNHVYKERFPKATQQMEERLQHFIHHFKNIPCDSLRLADPVVRFIHHQVVEMARDCLHKSHAKLITSRYFY

ELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEM
|.| || .||.|||.|||| | .|| ....|.||.|.||||||||||||||||| |||||||.||..||||||||||||.|||||.||.|||
EMQEKLERLLHEAHERSESEEVPFITHVIKKLLIIISRPARLLECLEFDPEEFYHLLEAAEGQAKVMQGIKTDIPRYIIHQLGLNRDPIEEM
Kinase MAST4.AA MAST 580-853 54 1.2e-229 709.71 In-house 1-337 (337) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-337/337
Sequence Identity: 54% (184 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFET--RRHLCMVMEYVEGGDCATLMKNMG-PLP
||.||.||.||||||| |.||....|||||.||.|..|..||..|.||||.|||...|||||.|..||.|  ...|||||||.||||||.|....| ..|
FEFIKCISKGAYGAVYLVKHKKTNDRFAMKVINCQDTIDKNQVDQVFVERNILTMYQNPFVVKMFYSFQTPCKKYLCMVMEYMEGGDCASLLRMYGNCMP

VDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEG---------------HIEKDAREFL------------
..||.||.|||||.|||||.||||||||||||.| .|.||||||||||||||.|..|||.|||               ||.|....|             
EWMAKMYIAETVLCLEYLHSYGIVHRDLKPDNMLIDSNGHIKLTDFGLSKVGFMDKTTNHYEGYFNTNIFGKNHFNEYHIQKNYQQFQQDFGINGNTNKN

------------------------DKQVCGTPEYIAPEVILRQG-YGKPVDWWAMGIILYEFLVGCVPFFGDTPEE-LFGQVIS--DEINWPEK-----D
                        .||.||||.||||||||.|| ||||||||.||||.|||||||.||.|||||| .|...|.  .||.|||.     |
QIQQQQEQPQCQQQNEMRPHNIHQNKQCCGTPDYIAPEVILGQGTYGKPVDWWSMGIIMYEFLVGCPPFNGDTPEEMIFQNIINHDREIEWPEEGDPNDD

EAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFF
.. .|.||.||  ||..||..|||..||.|.|||.||
DCMSPEAQDLIKKLLQPNPKKRLGANGADEIKQHPFF
Kinase MAST4.AA MAST 580-853 50 1.3e-210 685.62 In-house 1-334 (334) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-334/334
Sequence Identity: 50% (170 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDM
||.||.||.||||.|| |.||....|||||.|.|...|..|...| .|||.||....|||||.|..||.|...|||||||.||||||.|....|.....|
FEFIKPISKGAYGKVYLVKHKKTNDRFAMKVIKKSDMINKNMVDQVQVERNILAMYQNPFVVKMFYSFQTKNYLCMVMEYMEGGDCASLLHMYGYFDEWM

ARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEG---------------HIEKDAREFL---------------
|.||.|||||.|||||..|||||||||||.| .|.||||||||||||||.|......|||               ....|...|                
AKMYIAETVLCLEYLHSHGIVHRDLKPDNMLIDSNGHIKLTDFGLSKVGFMDDLNMMYEGYTPPNIFNNSTDPMNKNQQDYSQFPGDFGINGNTNKNQIQ

--------------------DKQVCGTPEYIAPEVILRQG-YGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDE---INWPEK-----DEAPP
                    .||.||||.||||||||.|| .|||||||.||||.|||||||.||..|||||.|.......   | |||.     |||..
QQQEQPQCQQQNMRPHNIHQNKQCCGTPDYIAPEVILGQGTHGKPVDWWSMGIIMYEFLVGCPPFNDDTPEEIFQNILNRNCNEIEWPEGGDINDDEAMS

PDAQELITLLLRQNPLERLGTG-GAYEVKQHRFF
|.||.||  || ..|..|||.. ||.|||||.||
PEAQDLIEKLLQPDPRKRLGANNGADEVKQHPFF
Kinase MAST4.AA AGC 580-853 28 2.1e-166 434.27 In-house 1-395 (395) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-395/395
Sequence Identity: 28% (115 aa)

FETIKLISNGAYGAVYFVRHK------ESRQRFAMKKINKQN-LILRN--QIQQAFVE------------RDILTFAE----------NPFVVS-MYCSF
|| .|.|. |..| || ||.|       . . .|||...|.   ....  ..... .|            |.||. .           .||.|. ...||
FEFLKVIGKGSFGKVYLVRKKDGCHKKDTGKYYAMKVLKKKHKIVIKKKNMVEHTMTEHWKQDQGYVWEERNILEKCNHTHNHPIKSHHPFIVKILHYSF

ETRRH--LCMVMEYVE--------GGDCATLMKNMG-----PLPVDMARMYFAE-------TVLALEYLHN-YGIVHRD-LKPDNLLVTSMGHIKLTDFG
.. .|  |..||||..        ||.| ... ..|      .. ..|| |.||       .||||||||.  ||.||| |||.|.| .. |||||||||
QDKDHTYLYFVMEYMPRYVNDLYCGGGCELFLHLQGRRKEGRFDEERARFYAAEMQKICGCIVLALEYLHSHMGIIHRDYLKPENILLDEDGHIKLTDFG

LSKVGLMSM-----------TTNLYEG---------------HIEKDA----------REFLDKQVCG--TPEYIAPEVILRQGYGKPVDWWA--MGIIL
|.|.| ...           ... ...               .. ...           ... .  ||  ||||.|||..    |||..|||.  .|...
LCKEGMNDGEKGQQSQSNMKFHW-HNKKYQKSNMDTNKCNPNNWQRNNMSQEDDTTRKKRDRTWTFCGPCTPEYMAPEIL----YGKACDWWSFGLGCLM

YEFLVGCVPFFGDTPE----------ELFGQVI------SDEINWP-------EKDEAPPPDAQELITLLLRQNPLERLGTG-------GAYEVKQHRFF
||.|.|. ||.||.|.          |.| ...      .... .|       ..|.   |.|. ||. ||  .| .|||.|       || |.|.| .|
YEMLTGYPPFYGDNPQENRHPFRNPMEMFQKIMNGRYFINWKVKFPFPEDPQFPEDRWFSPEAKDLIKKLLCKDPEKRLGCGGNDFGDKGAEEIKNHPWF
Kinase MAST4.AA S_TKc 580-853 32 2.5e-100 340.52 SMART 1-231 (231) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-231/231
Sequence Identity: 32% (94 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEG--GDCATLMKNMGP---
.| ...|  ||.| || .|||.. ...|.|.|.          ...  |..||     |..| .| .|..  ||.|||||..|  ||  ...|..|.   
YEILRKIGKGAFGKVYKCRHKKTGRIVAIKIIK----------EHIRREIQILKK-HHPNIVKLYDVFQD-DHLYMVMEYCDGDLGDLFDYIKKRGRHGL

---LPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYG
   .|  .|| |......||||.|. ||.||||||.|.|...  |||..||||...                       . .||||.|.||||.   |||
RFPFPE-HARFYMYQICCALEYCHSHGIIHRDLKPENILLDE--HIKICDFGLARQL----------------------TTFCGTPWYMAPEVL---GYG

K-PVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTGGAYE----VKQHRFF
| ..|||. |.||||.|.|. ||   .....| ..              .|.| ..|  .|. .| .|..   | .    .|.| .|
KCKCDWWSCGCILYEMLCGYPPFP--QMQMMFKKIG-------------SPEAKDFIRKCLQKDPEKRPTA-EALQDEWDIKCHPWF
Kinase MAST4.AA RSK-Unclassified 580-853 36 5.2e-91 286.08 In-house 1-283 (283) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-283/283
Sequence Identity: 36% (105 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMK--NMGPLPV
|. ..||  |  | || || |.. | .|||  .|. .| .| |. .  |..|.   . ||.| .| || |.  | ..|||  |||    .       .  
FIKHRLIGQGDFGKVYLVRKKDTQQIYAMKVLHKHKMIRHNKIKYVMTEQNIMATTNHPFIVTLYHSFQTQDYLYLCMEYCMGGDFFRHLQTRKEKCFCE

DMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVG------LMS--MTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQG
| .|.| .|...||||||  ... ||||||| .    ||..||||.||. .       |       . .  |  .   |  .  ||| ||.|||.| | |
DDVRFYASEVTAALEYLHKRDFIYRDLKPDNIVLHQEGHVMLTDFDLSIQAKDSKKPVMKGSAQSTYVDTKICDHG--FRTNSFCGTEEYLAPEMIRRCG

YGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTG-G-AYEVKQHRFF
 .| |||| .||..|| |.|| |. |.  .|.|  .   ..  |.  .    ....||  || .||  ||| . | | ..|.| ||
HTKAVDWWTLGIVMYEMLFGCPPYYGNNKNETFYNIEKNHVKFPHDSYI-SNNCRDLIKKLLQRNPAKRLGCKMGDADDIKRHPFF
Kinase MAST4.AA AktR 580-853 36 9.6e-86 291.17 In-house 1-268 (268) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-268/268
Sequence Identity: 36% (104 aa)

FETIKLISNGAYGAVYFVRHK-ESRQRFAMKKINKQNLILRNQIQQAFVERDILT-FAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPV
|. .|.. .||.| || |..| .. . .||| | |...| |||....  ||.|.   . .|| | .. .|.|...|  .|||..||.  .  |. . .  
FHVYKCLGKGAFGKVYLVQKKRDNQKQYAMKMIKKKKIIERNQMHHTITERNIMEMCCNHPFIVKLHYCFQTKEKLFFIMEYCPGGELFQHLKKKKKFSE

DMARMYFAETVLALEYLHNY-GIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDA-REFLDKQVCGTPEYIAPEVILR-QGYGKPV
..|| |||. .|||||||.   |. |||||.|.|  . ||.|.|||||||              ....  .. . . .||||||.|||.|.. .|..|.|
EQARFYFAQILLALEYLHQHMNIIYRDLKPENILLDEDGHVKITDFGLSK--------------LQFMMHYDEKAYSFCGTPEYMAPEMITKKKGHNKSV

DWWAMGIILYEFLVGCVPFFG-DTPEELFGQVISDEINWPEKDEAPPPDAQELIT-LLLRQNPLERLG--TGGAYEVKQHRFF
|||| || .|| ..|| ||.  .. .... . |.. .| ... . . . ...||.  || .|| .|||  . |. |.| | .|
DWWAFGIFIYEMVTGCPPFYHKNQNQMFQKIKIKQ-QNKFPYPSYFSSQCKDLIQRKLLCKNPNKRLGYYCNGWEEIKSHPWF
Kinase MAST4.AA Pkinase 580-853 28 1.7e-78 271.87 Pfam 1-293 (293) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-293/293
Sequence Identity: 28% (90 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFV--ERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGG----DCATLMK--N
..... |  |..| ||  .|| .....|.|.|.|.  .  .. |...   |  |.     |..| .|..||...|..|||||.|||    | .....  .
YHIGRKIGSGSFGCVYKCHHKGTGKIVAVKIIKKR-HEKSWKDQTHRRLSEIQIMKRLNHPNIVRLYDWFEDKDHIYMVMEYCEGGLYVMDLFDYISTWR

MGPL--PVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHI----KLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVI
 |..  . . ...|. ... .|||.|. ||.||||||.|.|. . |||    |..||||.|.              .   . .  . .||||.|.|||||
HGCFYFSEWECKFYMYQILRGLEYCHSMGIIHRDLKPENILIDNNGHIDACVKICDFGLAKQFD------------Y-NNS-M--TTFCGTPWYMAPEVI

L-RQGYGKPVDWWAMGIILYEFLVGCVPFFGD------TPEELFGQVIS--DEINWPEKDE----------------APPPDAQELITLLLRQNPLERLG
. .|.||..||.|..|.||||.| |  || |.      .. ... .. .   . .||....                . .......|. .|...| .|..
RGGQYYGPKVDMWSCGCILYEMLCGRPPFPGHCWHDNHDQMQMICRIMGPPLHFDWPWWCSISYFFFRHWHFHWTFHNWSEECKDFIKWCLCKDPKKRPT

TGGAYEVKQHRFF
   | ...|| .|
---AEQILQHPWF
Kinase MAST4.AA RSK 583-853 32 2.5e-78 266.51 In-house 1-278 (278) Show / Hide 
Range on Protein: 583-853
Range on HMM: 1-278/278
Sequence Identity: 32% (96 aa)

IKLISNGAYGAVYFVRHK---ESRQRFAMKKINKQNLILRNQ-IQQAFVERDILTFAE-NPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGP---
.|.   |.||.|. .| .    ..| .||| ..|  .. |.  .  .. ||.||   .  ||.|. . .|.|  .| ..|||..||.  |  .. .    
LKVLGQGGYGKVFLCRKCTHHDTGQIYAMKVLKKAKIVQRQKDTRHTMTERNILERCQQHPFIVTLHYAFQTEGKLYLIMEYCRGGELFTHLQKEKHFGC

LPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTS---MGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVIL----R
.  | |..|.|| |||.|.||  ||.||||||.| | ..    |||||||||..| .  ...  ..                |.| ||.|||..     .
FSEDEACFYMAEIVLAVEHLHQQGIIHRDLKPENILYDDESNEGHIKLTDFGFCKQFRCDHNQWTMT--------------FCYTIEYMAPEMLKRANQQ

QGYGKPVDWWAMGIILYEFLVGCVPFFG---DTPEELFGQVISDE--INWPEKDEAPPPDAQELITLLLRQNPLERLG--TGGAYEVKQHRFF
 |..| .|||  |...|| |.||.|| |   ....|.. .. . .   . |. ..   ..|..||. .|  .|  |||   ..| |.||| .|
GGHDKACDWWSLGVLMYEMLTGCPPFQGPNDNNRKEIMKRIQKGKFSLTMPNWQYL-SQEAKDLIRKMLHRDPHKRLGCGPQDAEEIKQHPWF
Kinase MAST4.AA NDR 580-729 45 1.4e-75 232.38 In-house 1-155 (350) Show / Hide 
Range on Protein: 580-729
Range on HMM: 1-155/350
Sequence Identity: 45% (71 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAE--NPFVVSMYCSFETRRHLCMVMEYVEGGDCAT---LMKNMGP
|| ||.|. ||.| |. ||.|.... .|||...|.  . .||. .   ||||.. |   ||.||..|.||    .|. ||||. |||  |   | ..|. 
FEIIKIIGKGAFGEVWLVRKKDTGHIYAMKIMRKSEMMWKNQVAHVRAERDIMAEADNKNPWVVKLYYSFQDKQYLYLVMEYHPGGDMMTYCYLLMKMDI

LPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGL
.. |.|| | ||.|.|.|..|..|..|||.||||.|. . ||||||||||.. |.
FTEDWARFYIAEMVCAIESVHKMGYIHRDIKPDNFLIDKDGHIKLTDFGLCTGGM
Kinase MAST4.AA Akt 580-731 38 9.5e-52 163.66 In-house 1-154 (264) Show / Hide 
Range on Protein: 580-731
Range on HMM: 1-154/264
Sequence Identity: 38% (59 aa)

FETIKLISNGAYGAVYFVRHKESRQRF-AMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVD
|. .|..  |..| |. |. | . . . ||| . |. .| .||..... || ||    .||.|... || |  .|..||.|. ||.  ....  .  . |
FDFLKVLGKGSFGKVFLVKKKDTQKWYYAMKVLKKDYIIQKNQVEHTKTERNILQHCNHPFIVNLHYSFQTQDKLYFVMDYCNGGELFYHLQRKRRFSED

MARMYFAETVLALEYLHNYGIVHRD-LKPDNLLVTSMGHIKLTDFGLSKVGLMS
 || | || .||.|.||. .|. || ||| |.|. . |||.||||||.| ....
RARFYCAEIILAIEHLHSKNIIYRDYLKPENILMDKDGHICLTDFGLCKENIIK
Kinase MAST4.AA PKA 580-726 42 1.2e-51 177.94 In-house 1-149 (263) Show / Hide 
Range on Protein: 580-726
Range on HMM: 1-149/263
Sequence Identity: 42% (64 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVV-SMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVD
||.|... .| .| |. .|||.| . .||| ..||  . ..|..    |. ||.  ..||.| .|| .| ..|.| ||||||.||.  . . . | .. .
FEIIQTLGTGSFGRVMLCRHKHSGNYYAMKMLKKQEIVRLKQVEHVHNEKNILQNIKHPFIVKKMYWTFQDDRYLYMVMEYVPGGEMFSYLRKSGRFSNP

MARMYFA-ETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSK
.|. | | . ||||||||.  |..||||| |.|  . ||||.|||| .|
HAKFYAASQIVLALEYLHSQNIIYRDLKPENILIDKNGHIKITDFGFAK
Kinase MAST4.AA SGK 580-726 36 5.5e-50 152.3 In-house 1-173 (283) Show / Hide 
Range on Protein: 580-726
Range on HMM: 1-173/283
Sequence Identity: 36% (63 aa)

FETIKLISNGAYGAVYFVRHKES------------------RQRFAMKKINKQNLI-------LRNQIQQAFVER-DILTFAENPFVVSMYCSFETRRHL
|. .|.|  | .| || |.||.                    . .||| ..||  |        ...      || .||     || |.   .| |   |
FDFLKVIGKGSFGKVYQVKHKDDETCQRVWLIFQSLLSQSEDKFYAMKVLQKQHIINEYRQDLKKKEQKHIMAERNVILKNMKHPFIVNLHYAFQTKWKL

CMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSK
 .||.||.||.    ..  . .| | || | || |.||||||   |..|||||.|.|  ..||..||||||.|
YFVMDYVNGGEMFYHLQREKHFPEDRARFYAAEIVCALEYLHSQDIIYRDLKPENILLDHQGHCCLTDFGLCK
Kinase MAST4.AA SGK 754-853 39 6.6e-41 123.76 In-house 186-283 (283) Show / Hide 
Range on Protein: 754-853
Range on HMM: 186-283/283
Sequence Identity: 39% (40 aa)

CGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLGTG-GAYEVKQHRF
|||||| ||||.. |.... |||| .|..|||...|  ||.    .... . |. ... |    .   || .||..||  .| .||||  .. |.|.| |
CGTPEYLAPEVLRKQPHDRTVDWWCLGCVLYEMMYGLPPFYSRNKQQMYDNIINQPLQIPPQMSN---DAWDLIQGLLQKDPKKRLGTKRDFMEIKNHPF

F
|
F
Kinase MAST4.AA Akt 753-853 36 5.2e-40 125.53 In-house 162-264 (264) Show / Hide 
Range on Protein: 753-853
Range on HMM: 162-264/264
Sequence Identity: 36% (38 aa)

VCGTPEYIAPEVILRQGYGKPVDWWA-MGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQELITLLLRQNPLERLG--TGGAYEVKQ
.||||||.|||... .|..|.||||.  |...||.. |  ||. .  ...| .  . ... |.| ..  |... ||. ||  .| .|||   ..| |.|.
FCGTPEYLAPEILNGNGHTKAVDWWGSLGCVMYEMMCGRPPFYNQDHHQMFKKIKNQPVKFPDK-DWISPECKDLISGLLNKDPKKRLGGGPNDAQEIKR

HRFF
| .|
HPWF
Kinase MAST4.AA NDR 755-853 40 4.8e-36 108.2 In-house 243-350 (350) Show / Hide 
Range on Protein: 755-853
Range on HMM: 243-350/350
Sequence Identity: 40% (44 aa)

GTPEYIAPEVILR-QGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPP------PDAQELITLLLRQNPLERLG-TG-GAY
|||.|.||||... .||.. .|||  | |.||.|||  ||..|||.| .  ... .. |..  .. |      |.|. ||. |.  .| .|||  | |. 
GTPDYMAPEVFQQKTGYNQTCDWWSLGCIMYEMLVGYPPFCSDTPQETCMKIMNWKQTWDWPMTHFPPEANLSPEAWDLIRRLCCPDPDHRLGSHGKGVD

EVKQHRFF
|.|.| ||
EIKSHPFF
Kinase MAST4.AA PKA 753-853 37 7.3e-34 118.82 In-house 158-263 (263) Show / Hide 
Range on Protein: 753-853
Range on HMM: 158-263/263
Sequence Identity: 37% (41 aa)

VCGTPEYIAPEVILRQ-GYGKPVDWWAMGIILYEFLVGCVPFFGD--TPEELFGQVISDEINWPEKDEAPPPDA---QELITLLLRQNPLERLGT--GGA
.||||||.|||.|..  |.||.||||| ||. ||.|.|  ||. .  .| ...   .. .|..|. .    .||     ||  ||  .. .|.|.  .|.
LCGTPEYLAPEIIQSKNGHGKSVDWWALGILIYEMLAGYPPFYDHNRDPMQIYEKICNGKIKFPRHFD---SDANYFKDLIKKLLQVDLTKRYGNMKNGV

YEVKQHRFF
  .| |..|
NDIKNHKWF
Kinase MAST4.AA TyrKc 580-853 19 3.6e-12 -25.94 SMART 1-280 (280) Show / Hide 
Range on Protein: 580-853
Range on HMM: 1-280/280
Sequence Identity: 19% (59 aa)

FETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFA--ENPFVVSMYCSFETR-------RHLCMVMEYVEGGDCATLMK
 . .| . .||.| ||  . |   .  | |....        ..    |  |..    ..| .| .|    .          |..||||...||.    .
ITLGKKLGEGAFGEVYKGTWK---IQVAVKMLKEDA------REDFMREARIMCKLGGHHPNIVRLYGVCTQQGRNFMYIEPLMIVMEYMPHGDLKDYLR

NMGP-LPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMS-MTTNLYEGHIEKDARE-FLDKQVCGTPEYIAPEVILR
. .| |...    .        ||||  ...||||   | ||.. ...| .|||||. .    ..    | .  ....|    | .       |||.|  
KHRPKLCMKDLLSFCWQIACGMEYLHSKNCIHRDLAARNCLVGENHTVKISDFGLSRDIYDDDQQG---ESKDYYRKKEHKGGKTMLPI-RWMAPECINY

QGYGKPVDWWAMGIILYEFLVGCVPFFGD------TPEELFGQVISDEINWPEKD-------EAPPPDAQELITLLLRQNPLERLGTGGAYEVKQHRFF
  .    | |. |. ..| .  . .   .      ...|.. .| . .   . ..       ..|.   | ...   . .| .|           |..|
GKFTTKSDVWSFGVCMWEIFTYGQQPYPGMIQQMMSNQEVIEYVKQGYRMPQPPNDYPMSFVCCPDEMYQ-IMKQCWHEDPEDRPTFSEI-----HECF
Kinase MAST4.AA Pkinase_Tyr 690-726 43 1.1e-05 19.89 Pfam 123-159 (270) Show / Hide 
Range on Protein: 690-726
Range on HMM: 123-159/270
Sequence Identity: 43% (16 aa)

ALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSK
..|||.. ...||||   | |||. ...| .||||..
GMEYLESKNCIHRDLAARNCLVTENNVVKICDFGLAR
Kinase MAST4.AA S_TK_X 854-918 19 0.000137 15.63 SMART 1-79 (79) Show / Hide 
Range on Protein: 854-918
Range on HMM: 1-79/79
Sequence Identity: 19% (17 aa)

RSLDWNSLLR----QKAEFIPQLE----SEDDTSYFD---TRSEKYHHMETEEEDDTNDEDFTVEIRQ-----------FSSCSHRF
|..||. |..     .. |.|..     |. |||.||   |. .          ..  |.  .  . |           |   . .|
RGIDWDKLYNQQKEIEPPFKPKIKEVYASPTDTSNFDPEFTEETPM--------LTPDDPPLSNSMWQNEMETEIPPDPFRGFTYVF
Kinase MAST4.AA Pkinase_Tyr 652-664 46 0.671292 3.67 Pfam 81-93 (270) Show / Hide 
Range on Protein: 652-664
Range on HMM: 81-93/270
Sequence Identity: 46% (6 aa)

HLCMVMEYVEGGD
....||||...||
QIYIVMEYMPHGD
PDZ MAST4.AA PDZ 1159-1239 24 1.3e-17 65.73 SMART 1-80 (80) Show / Hide 
Range on Protein: 1159-1239
Range on HMM: 1-80/80
Sequence Identity: 24% (23 aa)

KNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPAYQAG------LKAGDLITHING-EPVHGLVHTEVIELLLKSGN-----KVSITTTPFE
. .||.|.              .... .|..|.||..||      |..|| |. .|| .|| .. | .....| ..|.     ||..|  . .
GGLGFSIVG-------------GIFISSVVPGGPAEKAGRNTRGHLRPGDRILEVNGQQPVENMTHEQAVDMLKQCGGGGIGDKVTLTVQRPG
PDZ MAST4.AA PDZ 1186-1236 25 9.6e-10 35.93 Pfam 33-86 (86) Show / Hide 
Range on Protein: 1186-1236
Range on HMM: 33-86/86
Sequence Identity: 25% (14 aa)

NVEEG-SPAYQAG-LKAGDLITHINGEPVHGLVHTEVIELLLKSGN-KVSITTT
.| .| ..| ..| ...|| |. |||... .. | ...... .|.. .|..|  
EVMPGSGAAERGGRIHEGDQIISINGQDIRNMTHDRAVQAIKGSKGGEVHLTIQ