RAF1_Hsap_RAF -----------VML-STRIGSGSFGTVYKGKWHGDVAVK-I--------LKVVDPTP--E
KSR2_Hsap_KSR -----------LEI-GELIGKGRFGQVYHGRWHGEVAIR-L--------IDIERDNE--D
KSR1_Hsap_KSR -----------VEL-GEPIGQGRWGRVHRGRWHGEVAIR-L--------LEMDGHNQ--D
phl_Dmel_RAF -----------ILI-GPRIGSGSFGTVYRAHWHGPVAVK-T--------LNVKTPSP--A
ksr_Dmel_KSR -----------LLL-LERIGQGRFGTVHRALWHGDVAVK-L--------LNEDYLQD-EH
KSR2_Mmus_KSR -----------LEI-GELIGKGRFGQVYHGRWHGEVAIR-L--------IDIERDNE--D
KSR1_Mmus_KSR -----------VEL-GEPIGQGRWGRVHRGRWHGEVAIR-L--------LEMDGHNQ--D
BRAF_Mmus_RAF -----------ITV-GQRIGSGSFGTVYKGKWHGDVAVK-M--------LNVTAPTP--Q
ARAF_Mmus_RAF -----------VQL-LKRIGTGSFGTVFRGRWHGDVAVK-V--------LKVAQPTA--E
lin-45_Cele_RAF -----------FII-QYKVGSGSFGTVYRGEFFGTVAIK-K--------LNVVDPTP--S
ksr-2_Cele_RAF TWEDVTIKLTDVDV-MTKIGDGRFGSVYFGGYHGNAAVR-F--------VNMNYLSQEDR
ksr-1_Cele_KSR -----------FDKQAPIIGRGRFGKVLRGFHYGDVAVK-V--------YTMEHISDASK
BRAF_Hsap_RAF -----------ITV-GQRIGSGSFGTVYKGKWHGDVAVK-M--------LNVTAPTP--Q
ARAF_Hsap_RAF -----------VQL-LKRIGTGSFGTVFRGRWHGDVAVK-V--------LKVSQPTA--E
RAF1_Mmus_RAF -----------VML-STRIGSGSFGTVYKGKWHGDVAVK-I--------LKVVDPTP--E
KSR1_Spur_KSR ------------------------------------------------------------
Raf_Spur_RAF -----------IRL-GSRIGAGSFGTVFSGQWHGSVAVK-R--------LNVKDPTP--S
KSR2_Spur_KSR -----------LKY-GRCIEKGKLGSTYKGHWHGEVLIQ-T--------R---YSKS--T
AqueK675_Aque_KSR -------------------------------------------------------RQ--E
AqueK676_Aque_KSR -----------LKL-LEKLGSGPVAEVYKGYWHGEVAVK-R--------FVLPNATP--K
AqueK677_Aque_RAF -----------IEV-GERIGSGSFGTVYKGKWHGPVAVK-K--------LNVSNPTE--Q
AqueK678_Aque_RAF -----------IEV-GERIGSGSFGTVYKGKWHGPVAVK-K--------LNVSNPTE--Q
AqueK679_Aque_RAF -----------TIL-KETLGNGAYGVVYKAKCDELICAAKYMHPIFFALSEDPAV--NVV
RAF1_Hsap_RAF QFQAFRNEV-AVLRKTRHVNILLFMGYM-TK---DNLAIVTQWCEGS-SLYKHLHVQ-ET
KSR2_Hsap_KSR QLKAFKREV-MAYRQTRHENVVLFMGACMSP---PHLAIITSLCKGR-TLYSVVRDA-KI
KSR1_Hsap_KSR HLKLFKKEV-MNYRQTRHENVVLFMGACMNP---PHLAIITSFCKGR-TLHSFVRDP-KT
phl_Dmel_RAF QLQAFKNEV-AMLKKTRHCNILLFMGCV-SK---PSLAIVTQWCEGS-SLYKHVHVS-ET
ksr_Dmel_KSR MLETFRSEV-ANFKNTRHENLVLFMGACMNP---PYLAIVTSLCKGN-TLYTYIHQR-RE
KSR2_Mmus_KSR QLKAFKREV-MAYRQTRHENVVLFMGACMSP---PHLAIITSLCKGR-TLYSVVRDA-KI
KSR1_Mmus_KSR HLKLFKKEV-MNYRQTRHENVVLFMGACMNP---PHLAIITSFCKGR-TLHSFVRDP-KT
BRAF_Mmus_RAF QLQAFKNEV-GVLRKTRHVNILLFMGYS-TK---PQLAIVTQWCEGS-SLYHHLHII-ET
ARAF_Mmus_RAF QAQAFKNEM-QVLRKTRHVNILLFMGFM-TR---PGFAIITQWCEGS-SLYHHLHVA-DT
lin-45_Cele_RAF QMAAFKNEV-AVLKKTRHLNVLLFMGWV-RE---PEIAIITQWCEGS-SLYRHIHVQ-EP
ksr-2_Cele_RAF RADVFATEIVSAykNSRHDHIALFYGYVSDP-VTNTYAIVTNFYQHN-TLYHRIHEQLSE
ksr-1_Cele_KSR KAEEFKLEV-SAYKNTRHDNIALFLGYFMSD---GQYGMVMSLSKGSQSLYTLLHVV-RE
BRAF_Hsap_RAF QLQAFKNEV-GVLRKTRHVNILLFMGYS-TK---PQLAIVTQWCEGS-SLYHHLHII-ET
ARAF_Hsap_RAF QAQAFKNEM-QVLRKTRHVNILLFMGFM-TR---PGFAIITQWCEGS-SLYHHLHVA-DT
RAF1_Mmus_RAF QLQAFRNEV-AVLRKTRHVNILLFMGYM-TK---DNLAIVTQWCEGS-SLYKHLHVQ-ET
KSR1_Spur_KSR ------------------------------------------LCKGQ-TLYTHIHLR-KD
Raf_Spur_RAF QLQAFKNEV-AVLRKTRHANILLFMGCT-SK---PQLAIVTQWCEGS-SLYKHLHVL-DT
KSR2_Spur_KSR DINDFLDEV-AMLSKIRHENIALFMGACVEP---GNLAVVNSIHKGP-SLYHHLHVQ-RR
AqueK675_Aque_KSR DVRSFANEV-NTLCCIRHENIQLFMGVCLDLPAGDTFGIIMSEVKGD-TLYNHIHKL-GH
AqueK676_Aque_KSR QINKFREEV-AVLKKIRHENLALFMGVCLTT---PNLAIITSYCKGY-TLYKLLHYW-SE
AqueK677_Aque_RAF QMQAFKNEV-AVLMKTRHANILLFMGWT-SK---PRLTIVTQWCDGS-TLFSHIHVL-ED
AqueK678_Aque_RAF QMQAFKNEV-AVLMKTRHANILLFMGWT-SK---PRLTIVTQWCDGS-TLFSHIHVL-ED
AqueK679_Aque_RAF ATDKFKKEC-QLLSRLKHPNIVQYLGTH-EVNGI-DTVVLLMELMDS-SLHAYLHAHVDS
:*. ::
RAF1_Hsap_RAF --KFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHE---GL-TVKIGDFGLATV
KSR2_Hsap_KSR --VLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDN----G-KVVITDFGLFSI
KSR1_Hsap_KSR --SLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDN----G-KVVITDFGLFGI
phl_Dmel_RAF --KFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHE---DL-SVKIGDFGLATA
ksr_Dmel_KSR --KFAMNRTLLIAQQIAQGMGYLHAREIIHKDLRTKNIFIEN----G-KVIITDFGLFSS
KSR2_Mmus_KSR --VLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDN----G-KVVITDFGLFSI
KSR1_Mmus_KSR --SLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDN----G-KVVITDFGLFGI
BRAF_Mmus_RAF --KFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHE---DL-TVKIGDFGLATV
ARAF_Mmus_RAF --RFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHE---GL-TVKIGDFGLATV
lin-45_Cele_RAF RVEFEMGAIIDILKQVSLGMNYLHSKNIIHRDLKTNNIFLMD---DMSTVKIGDFGLATV
ksr-2_Cele_RAF --DFDQSWTFQISLQICQAMSYLHKKKILHRDLRTKNILLDN---PN-RVVVTDFALMKL
ksr-1_Cele_KSR --KLDLATTRKIAQQICQAVSYLHTKKILHKDLRSKNILLES---KN-KVVITDFGILSM
BRAF_Hsap_RAF --KFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHE---DL-TVKIGDFGLATV
ARAF_Hsap_RAF --RFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHE---GL-TVKIGDFGLATV
RAF1_Mmus_RAF --KFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHE---GL-TVKIGDFGLATV
KSR1_Spur_KSR --RFNINRTIQIATQITQGMSYLHHKGIVHKDLKSKNIFIEN----G-KVVLTDFALFSI
Raf_Spur_RAF --KLVMHQLIDISRQTAQGMDYLHAKNIIHRDLKSNNIFLHD---DL-TVKIGDFGLATV
KSR2_Spur_KSR --KLQTSSKMDIARQIGQAMGYLQAKGIIVGHLSSRCVYLES---KV-KLSVTRVDTSDV
AqueK675_Aque_KSR --NFTTPSKLKILRQISEGMEYLHAKGIQHGGLSSKLVTINH---R-VCISLS-F-----
AqueK676_Aque_KSR --AFSLERLANIGRQIAQAMGYLHARGIIHKGLNTKNIFLEKRDQKE-KVIITDMGLSSL
AqueK677_Aque_RAF --RFEMYKIIDICRQTAQGVDYLHAKQIIHRDLKSNNIFLHD---DM-TVKIGDFGLATV
AqueK678_Aque_RAF --RFEMYKIIDICRQTAQGVDYLHAKQIIHRDLKSNNIFLHD---DM-TVKIGDFGLATV
AqueK679_Aque_RAF --PLPFHQEINISLDVARALDYLHVNNIHHRDLSAKNILMLG---DY-RAKVSDFGQSKL
: : : .: **: . * : :. : : .
RAF1_Hsap_RAF KSRW---SG--SQQVEQPTGSVLWMAPEVIRMQ-----------DNNP--FSFQSDVYSY
KSR2_Hsap_KSR SGVLQAGR--REDKLRIQNGWLCHLAPEIIRQLSPDT-----EEDKLP--FSKHSDVFAL
KSR1_Hsap_KSR SGVVREGR--RENQLKLSHDWLCYLAPEIVREMTPGK-----DEDQLP--FSKAADVYAF
phl_Dmel_RAF KTRW---SG--EKQANQPTGSILWMAPEVIRMQ-----------ELNP--YSFQSDVYAF
ksr_Dmel_KSR TKLLYC-----DMGLGVPHNWLCYLAPELIRALQPEK----PRGECLE--FTPYSDVYSF
KSR2_Mmus_KSR SGVLQAGR--RDDKLRIQNGWLCHLAPEIIRQLSPDT-----EEDKLP--FSKHSDVFAL
KSR1_Mmus_KSR SGVVREER--RENQLKLSHDWLCYLAPEIVREMIPGR-----DEDQLP--FSKAADVYAF
BRAF_Mmus_RAF KSRW---SG--SHQFEQLSGSILWMAPEVIRMQ-----------DKNP--YSFQSDVYAF
ARAF_Mmus_RAF KTRW---SG--AQPLEQPSGSVLWMAAEVIRMQ-----------DPNP--YSFQSDVYAY
lin-45_Cele_RAF KTKWTV-NG--GQQQQQPTGSILWMAPEVIRMQ-----------DDNP--YTPQSDVYSF
ksr-2_Cele_RAF ERLENP-R--RNCTLLIPNHWIDYLSPEIAGNLMIDWRGDVLFQHELP--FSQESDVYSF
ksr-1_Cele_KSR KRLAHP-K--QKSGYLTSKFWTNYIAPELAMAMRTEYDE--YECDDFP--FSENSDVYAF
BRAF_Hsap_RAF KSRW---SG--SHQFEQLSGSILWMAPEVIRMQ-----------DKNP--YSFQSDVYAF
ARAF_Hsap_RAF KTRW---SG--AQPLEQPSGSVLWMAAEVIRMQ-----------DPNP--YSFQSDVYAY
RAF1_Mmus_RAF KSRW---SG--SQQVEQPTGSVLWMAPEVIRMQ-----------DDNP--FSFQSDVYSY
KSR1_Spur_KSR GRLSKQKT--RKDSLVIPDGWLCYLAPELITSLKINTDG--TQDMELP--FSEKSDVYSF
Raf_Spur_RAF KSRW---SG--SQQFEQPSGSILWMAPEVIRMR-----------DTNP--YSFQSDVYAF
KSR2_Spur_KSR T-----CQRA--SHACLPRGKLQYLAPELLAKARVVPP---EFITGNE--QTHSTDVYAF
AqueK675_Aque_KSR -----HN--TNNR--QLEGCDLPYLTPEEIRSITQLSNG--YLFLP--TKPTPSTDIFAF
AqueK676_Aque_KSR SSLLYDNTTSKGGVVTFPRAQLYSLAPEIIRKISPTPV---VQIDQEPYSYNYATDVYAF
AqueK677_Aque_RAF KTRW---DG--NEKVRQPTGSILWMAPEVIRME-----------EPDP--YTFYCDVYSF
AqueK678_Aque_RAF KTRW---DG--NEKVRQPTGSILWMAPEVIRME-----------EPDP--YTFYCDVYSF
AqueK679_Aque_RAF RNPNG---G--FSSNSPCPGNILYMPPETLKV-------------PPD--FAPTLDEFSF
:..* * ::
RAF1_Hsap_RAF GIVLYELMTGELPYSHIN-----NRDQIIFMVGRGYA-SPDLSK----L-YKNCPKAMKR
KSR2_Hsap_KSR GTIWYELHAREWPFKTQP------AEAIIWQMGTG--MKPNLSQ-------IGMGKEISD
KSR1_Hsap_KSR GTVWYELQARDWPLKNQA------AEASIWQIGSGEGMKRVLTS-------VSLGKEVSE
phl_Dmel_RAF GIVMYELLAECLPYGHIS-----NKDQILFMVGRGLL-RPDMSQ----V-RSDAPQALKR
ksr_Dmel_KSR GTVWYELICGEFTFKDQP------AESIIWQVGRG--MKQSLAN-------LQSGRDVKD
KSR2_Mmus_KSR GTIWYELHAREWPFKTQP------AEAIIWQMGTG--MKPNLSQ-------IGMGKEISD
KSR1_Mmus_KSR GTVWYELQARDWPFKHQP------AEALIWQIGSGEGVRRVLAS-------VSLGKEVGE
BRAF_Mmus_RAF GIVLYELMTGQLPYSNIN-----NRDQIIFMVGRGYL-SPDLSK----V-RSNCPKAMKR
ARAF_Mmus_RAF GVVLYELMTGSLPYSHIG-----SRDQIIFMVGRGYL-SPDLSK----I-FSNCPKAMRR
lin-45_Cele_RAF GICMYEILSSHLPYSNIN-----NRDQILFMVGRGYL-RPDRSK----I-RHDTPKSMLK
ksr-2_Cele_RAF GTIFFELLLRRMPTGCDS------WDQKLYAKMCG--QKAALQRLDA-Q-LQKIDGKLHE
ksr-1_Cele_KSR GCVWFEMLTGALPYAGEL------PHQILFAKTQG--IRPVLPN-------VKCTQELKE
BRAF_Hsap_RAF GIVLYELMTGQLPYSNIN-----NRDQIIFMVGRGYL-SPDLSK----V-RSNCPKAMKR
ARAF_Hsap_RAF GVVLYELMTGSLPYSHIG-----CRDQIIFMVGRGYL-SPDLSK----I-SSNCPKAMRR
RAF1_Mmus_RAF GIVLYELMAGELPYAHIN-----NRDQIIFMVGRGYA-SPDLSR----L-YKNCPKAMKR
KSR1_Spur_KSR GTIWYELLAGEWPFHKQP------VETVIWQVGKG--LKQPLNH-------LQASRDVKE
Raf_Spur_RAF GVVLFELVTQSLPYQNIK-----HKDQIIWMVGRGYL-QPDLSK----V-RNDTPKALKR
KSR2_Spur_KSR GTILFEIIAGRWPLQYAC------SESVIYQTCSG--RRDDFRN-------VTCSSTMKN
AqueK675_Aque_KSR GTIAYELTTLKLPFLNQS------LLDLIWSVGNNG-HQ-SLK---L-L-P---RSCFRS
AqueK676_Aque_KSR GTILYELITKRFPFSTDSLGFAYEFEVVIFLVGHG--HRQPFKG--------ETPKKLKD
AqueK677_Aque_RAF GVVVYELISGQLPYPHVQ-----DRDQILFMVGRGFL-RPDPSS----A-RKDTPKSFKQ
AqueK678_Aque_RAF GVVVYELISGQLPYPHVQ-----DRDQILFMVGRGFL-RPDPSS----A-RKDTPKSFKQ
AqueK679_Aque_RAF GIVTIQIMSRKDPKPSDL-----HRTG--SLIGTLVE-VPEIERRRDDIDSCDPSNPILG
* :: . .
RAF1_Hsap_RAF LVADCVKKVKEERPLFPQILSSI-----------------------EL
KSR2_Hsap_KSR ILLFCWAFEQEERPTFTKLM--------------------------DM
KSR1_Hsap_KSR ILSACWAFDLQERPSFSLLM--------------------------DM
phl_Dmel_RAF LAEDCIKYTPKDRPLFRPLL--------------------------NM
ksr_Dmel_KSR LLMLCWTYEKEHRPQFARLLS-------------------------LL
KSR2_Mmus_KSR ILLFCWAFEQEERPTFTKLM--------------------------DM
KSR1_Mmus_KSR ILSACWAFDLQERPSFSLLM--------------------------DM
BRAF_Mmus_RAF LMAECLKKKRDERPLFPQILASI-----------------------EL
ARAF_Mmus_RAF LLTDCLKFQREERPLFPQILA-------------------------TI
lin-45_Cele_RAF LYDNCIMFDRNERPVFGEVLE-------------------------RL
ksr-2_Cele_RAF LLLECWSSQPEKRPSFQQIVKRITV------------------QMPRK
ksr-1_Cele_KSR LLVSCWNTAPQDRPTLTDINLKLTALP----------KKPRVNRSPSF
BRAF_Hsap_RAF LMAECLKKKRDERPLFPQILASI-----------------------EL
ARAF_Hsap_RAF LLSDCLKFQREERPLFPQILA-------------------------TI
RAF1_Mmus_RAF LVADCVKKVKEERPLFPQILSSI-----------------------EL
KSR1_Spur_KSR ILMMCWSYSPDSRPIFQHIKK-------------------------TI
Raf_Spur_RAF LITDCYALNRDERPLFPVVLA-------------------------AL
KSR2_Spur_KSR LIQECWSQNPNERPSFQDIVKELKKNVSL-----------GNMKHSLS
AqueK675_Aque_KSR VIHRCWSH--TRRTTFKDILSSI-----------------------QD
AqueK676_Aque_KSR IVWECWSHQPQDRPPFSDLQERLARLCRKHTPITRSPSHPINLSKSVD
AqueK677_Aque_RAF LCIKCCQFEKDDRPLFHQILADL-----------------------ES
AqueK678_Aque_RAF LCIKCCQFEKDDRPLFHQVYNNL----------------------ESL
AqueK679_Aque_RAF LSISCISNDPQERPPIERMCQLL----------------------SLL
: * *. : :